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Literature summary for 1.7.3.3 extracted from

  • Dmytruk, K.; Smutok, O.; Dmytruk, O.; Schuhmann, W.; Sibirny, A.
    Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor (2011), BMC Biotechnol., 11, 58.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis development of an urate-selective microbial biosensor cells of the recombinant thermotolerant methylotrophic yeast Hansenula polymorpha as biorecognition element. The UOX producing cells are coupled to horseradish peroxidase and immobilized on graphite electrodes by physical entrapment behind a dialysis membrane. A high urate selectivity with a detection limit of about 8 microM is found Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Pichia angusta Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.067
-
wild-type, pH 8.9, 25°C Ogataea angusta
2.67
-
recombinant strain overexpressing the enzyme, pH 8.9, 25°C Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Ogataea angusta 5-hydroxyisourate + H2O2
-
?

Synonyms

Synonyms Comment Organism
Uox
-
Ogataea angusta