Application | Comment | Organism |
---|---|---|
analysis | development of a high-throughput screening system of Bacillus fastidiosus uricase mutants, using 96-well plates and monitoring of uric acid by absorbance at 298 nm | Metabacillus fastidiosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Metabacillus fastidiosus |
Protein Variants | Comment | Organism |
---|---|---|
L171I/Y182F/Y187F/A193S | mutant displays higher activity and lower thermostability | Metabacillus fastidiosus |
V144A | mutant displays higher activity and consistent thermostability | Metabacillus fastidiosus |
General Stability | Organism |
---|---|
half-life of wild-type at pH 7.4, 37°C is 22 days, half-life of mutant L1711I/Y182F/Y187F/A193S is 0.3 days | Metabacillus fastidiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
Urate | mutant L1711I/Y182F/Y187F/A193S, pH 9.2, 27°C | Metabacillus fastidiosus | |
0.31 | - |
Urate | wild-type, pH 9.2, 27°C | Metabacillus fastidiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metabacillus fastidiosus | C5HDG5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.8 | - |
wild-type, pH 9.2, 27°C | Metabacillus fastidiosus |
8.3 | - |
mutant L1711I/Y182F/Y187F/A193S, pH 9.2, 27°C | Metabacillus fastidiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Metabacillus fastidiosus | 5-hydroxyisourate + H2O2 | - |
? |