Information on EC 3.6.1.12 - dCTP diphosphatase

Word Map on EC 3.6.1.12
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.1.12
-
RECOMMENDED NAME
GeneOntology No.
dCTP diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dCTP + H2O = dCMP + diphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
pyrimidine deoxyribonucleotides dephosphorylation
-
-
Pyrimidine metabolism
-
-
pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
dCTP nucleotidohydrolase
Also hydrolyses dCDP to dCMP and phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-87-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
subsp. mycoides
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-dATP + H2O
2-chloro-AMP + diphosphate
show the reaction diagram
-
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-AMP + diphosphate
show the reaction diagram
-
-
-
?
5-bromo-dCTP + H2O
5-bromo-dCMP + diphosphate
show the reaction diagram
5-chloro-dCTP + H2O
5-chloro-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-fluoro-dUTP + H2O
5-fluoro-dUMP + diphosphate
show the reaction diagram
-
-
-
?
5-formyl-dCTP + H2O
5-formyl-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-hydroxy-dCTP + H2O
5-hydroxy-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-hydroxymethyl-dCTP + H2O
5-hydroxymethyl-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-iodo-dCTP + H2O
5-iodo-dCMP + diphosphate
show the reaction diagram
5-methyl-dCTP + H2O
5-methyl-dCMP + diphosphate
show the reaction diagram
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
dATP + H2O
dAMP + diphosphate
show the reaction diagram
dCDP + H2O
?
show the reaction diagram
-
-
-
-
?
dCTP + H2O
dCMP + diphosphate
show the reaction diagram
dTTP + H2O
dTMP + diphosphate
show the reaction diagram
dTTP + H2O
dTTP + diphosphate
show the reaction diagram
-
-
-
-
?
dUTP + H2O
dUMP + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dCTP + H2O
dCMP + diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
maximum activity in presence of 100 mM MgCl2
MnCl2
up to 40% of the activity with MgCl2
additional information
divalent cation is absolutely required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14-alpha-triptolide
-
-
5-hydroxytriptonide
-
-
dCDP
-
competitive inhibition of hydrolysis of dCTP
dCMP
-
competitive inhibition of hydrolysis of dCTP and dCDP
dCTP
-
competitive inhibition of hydrolysis of dCDP
triptolide
-
noncompetitive inhibition
triptonide
-
-
triptriolide
-
-
additional information
not inhibitory: ITP up to 0.5 mM
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.107
2-chloro-dATP
pH 8.0, 30°C
0.169
2-hydroxy-dATP
pH 8.0, 30°C
0.0217 - 0.109
5-bromo-dCTP
0.05
5-chloro-dCTP
pH 8.0, 30°C
0.034
5-fluoro-dUTP
pH 8.0, 30°C
0.0092
5-formyl-dCTP
-
pH 8.0, 25°C
-
0.164
5-hydroxy-dCTP
pH 8.0, 30°C
0.0629
5-hydroxymethyl-dCTP
-
pH 8.0, 25°C
0.0039 - 0.239
5-iodo-dCTP
0.0333 - 0.085
5-Methyl-dCTP
0.529
CTP
pH 8.0, 30°C
0.0793 - 0.293
dATP
0.0441 - 0.152
dCTP
0.065 - 0.407
dTTP
0.24 - 0.484
dUTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.57
2-chloro-dATP
Mus musculus
Q9QY93
pH 8.0, 30°C
1.39
2-hydroxy-dATP
Mus musculus
Q9QY93
pH 8.0, 30°C
0.505 - 9.3
5-bromo-dCTP
1.5
5-chloro-dCTP
Mus musculus
Q9QY93
pH 8.0, 30°C
0.58
5-fluoro-dUTP
Mus musculus
Q9QY93
pH 8.0, 30°C
3
5-formyl-dCTP
Homo sapiens
-
pH 8.0, 25°C
-
0.87
5-hydroxy-dCTP
Mus musculus
Q9QY93
pH 8.0, 30°C
5.6
5-hydroxymethyl-dCTP
Homo sapiens
-
pH 8.0, 25°C
0.000864 - 6.2
5-iodo-dCTP
0.362 - 4.8
5-Methyl-dCTP
0.31
CTP
Mus musculus
Q9QY93
pH 8.0, 30°C
0.14 - 2
dATP
0.367 - 5.7
dCTP
0.108 - 1.8
dTTP
0.081 - 0.76
dUTP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14.7
2-chloro-dATP
Mus musculus
Q9QY93
pH 8.0, 30°C
42482
8.22
2-hydroxy-dATP
Mus musculus
Q9QY93
pH 8.0, 30°C
2804
4.63 - 256
5-bromo-dCTP
42484
30
5-chloro-dCTP
Mus musculus
Q9QY93
pH 8.0, 30°C
42485
1.9
5-fluoro-dUTP
Mus musculus
Q9QY93
pH 8.0, 30°C
42486
322
5-formyl-dCTP
Homo sapiens
-
pH 8.0, 25°C
206949
5.3
5-hydroxy-dCTP
Mus musculus
Q9QY93
pH 8.0, 30°C
42483
90
5-hydroxymethyl-dCTP
Homo sapiens
-
pH 8.0, 25°C
5295
4.56 - 371
5-iodo-dCTP
19681
4.25 - 143
5-Methyl-dCTP
3059
0.156
CTP
Mus musculus
Q9QY93
pH 8.0, 30°C
60
0.483 - 25
dATP
140
2.4 - 119
dCTP
179
0.551 - 28
dTTP
145
0.337 - 1.57
dUTP
435
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.356
5-hydroxytriptonide
-
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.168
triptolide
-
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.135
triptonide
-
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.085
triptriolide
-
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 11
-
more than 80% of maximum activity
8 - 10
-
pH 8.0: about 55% of maximal activity, pH 10.0: about 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
temperature-dependent increase of activity up to 60°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Manually annotated by BRENDA team
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Manually annotated by BRENDA team
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
exclusivelyx located in the cytosol
Manually annotated by BRENDA team
-
enzyme accumulates in the nucleus of tumor cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
2 * 18000, SDS-PAGE
18783
x * 19000, SDS-PAGE, x * 18783, calculated
19000
x * 19000, SDS-PAGE, x * 18783, calculated
20800
-
x * 20800, recombinant protein with His-tag, SDS-PAGE
59000
-
dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
2 * 18000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
generation of 3D models of DCTPP1 and its 5-methyl-dCTP-bound tetramer based on X-ray crystal structure of the tetrameric mouse dCTPase 1, PDB entry 2OIG. The cytosines of substrates 5-methyl-dCTP and dCTP bind deeply into the substrate pocket, which is composed of the residues from the dimer interface, including His38, Trp47, Glu63, Glu66, Trp73, Glu95, Asp98 and Tyr102
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glutathione-Sepharose column chromatography and Mono Q column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 Star (DE3) cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
neither knockdown nor over-expression of DCTPP1 significantly modifies the fraction of anti-BrdU reactive cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E63Q
-
catalytically inactive
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
enzyme accumulates in the nucleus of cancer cells compared to the paired adjacent tissue cells in multiple carcinomas including lung, breast, liver, cervical, gastric and esophagus cancer. The higher expression in the nucleus of lung, gastric and esophagus cancer cells is associated with histological subtypes. The nucleic accumulation is apparently observed as well when tumor cell line MCF-7 is treated with H2O2 in vitro
Show AA Sequence (457 entries)
Please use the Sequence Search for a specific query.