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Literature summary for 3.6.1.12 extracted from

  • Requena, C.E.; Perez-Moreno, G.; Ruiz-Perez, L.M.; Vidal, A.E.; Gonzalez-Pacanowska, D.
    The NTP pyrophosphatase DCTPP1 contributes to the homoeostasis and cleansing of the dNTP pool in human cells (2014), Biochem. J., 459, 171-180.
    View publication on PubMed
Search for more literature for 3.6.1.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
E63Q catalytically inactive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
 5-formyl-dCTP pH 8.0, 25°C Homo sapiens
0.0168
-
 5-iodo-dCTP pH 8.0, 25°C Homo sapiens
0.0333
-
 5-Methyl-dCTP pH 8.0, 25°C Homo sapiens
0.0365
-
 5-bromo-dCTP pH 8.0, 25°C Homo sapiens
0.0476
-
 dCTP pH 8.0, 25°C Homo sapiens
0.0629
-
 5-hydroxymethyl-dCTP pH 8.0, 25°C Homo sapiens
0.065
-
 dTTP pH 8.0, 25°C Homo sapiens
0.0793
-
 dATP pH 8.0, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20800
-
 x * 20800, recombinant protein with His-tag, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H773
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-
MRC-5 cell
-
 Homo sapiens
-
TK-6 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromo-dCTP + H2O
-
 Homo sapiens 5-bromo-dCMP + diphosphate
-
 ?
5-formyl-dCTP + H2O
-
 Homo sapiens 5-formyl-dCMP + diphosphate
-
 ?
5-hydroxymethyl-dCTP + H2O
-
 Homo sapiens 5-hydroxymethyl-dCMP + diphosphate
-
 ?
5-iodo-dCTP + H2O
-
 Homo sapiens 5-iodo-dCMP + diphosphate
-
 ?
5-methyl-dCTP + H2O
-
 Homo sapiens 5-methyl-dCMP + diphosphate
-
 ?
dATP + H2O
-
 Homo sapiens dAMP + diphosphate
-
 ?
dCTP + H2O
-
 Homo sapiens dCMP + diphosphate
-
 ?
dTTP + H2O
-
 Homo sapiens dTMP + diphosphate
-
 ?
additional information no substrtaes: ATP, CTP, UTP, dCDP, 5-methyl-dCDP, dGTP. Poor substrate: dUTP Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 20800, recombinant protein with His-tag, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
XTP3-TPA
-
 Homo sapiens
XTP3-transactivated protein A
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 dTTP pH 8.0, 25°C Homo sapiens
2
-
 dATP pH 8.0, 25°C Homo sapiens
3
-
 5-formyl-dCTP pH 8.0, 25°C Homo sapiens
4.8
-
 5-Methyl-dCTP pH 8.0, 25°C Homo sapiens
5.6
-
 5-hydroxymethyl-dCTP pH 8.0, 25°C Homo sapiens
5.7
-
 dCTP pH 8.0, 25°C Homo sapiens
6.2
-
 5-iodo-dCTP pH 8.0, 25°C Homo sapiens
9.3
-
 5-bromo-dCTP pH 8.0, 25°C Homo sapiens

General Information

General Information Comment Organism
physiological function DCTPP1-deficient cells accumulate high levels of dCTP and are hypersensitive to exposure to the nucleoside analogues 5-iodo-2'-deoxycytidine and 5-methyl-2'-deoxycytidine Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25
-
 dATP pH 8.0, 25°C Homo sapiens
28
-
 dTTP pH 8.0, 25°C Homo sapiens
90
-
 5-hydroxymethyl-dCTP pH 8.0, 25°C Homo sapiens
119
-
 dCTP pH 8.0, 25°C Homo sapiens
143
-
 5-Methyl-dCTP pH 8.0, 25°C Homo sapiens
256
-
 5-bromo-dCTP pH 8.0, 25°C Homo sapiens
322
-
 5-formyl-dCTP pH 8.0, 25°C Homo sapiens
371
-
 5-iodo-dCTP pH 8.0, 25°C Homo sapiens