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Literature summary for 3.6.1.12 extracted from
- Human dCTP pyrophosphatase 1 promotes breast cancer cell growth and stemness through the modulation on 5-methyl-dCTP metabolism and global hypomethylation (2015), Oncogenesis, 4, e159.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| generation of 3D models of DCTPP1 and its 5-methyl-dCTP-bound tetramer based on X-ray crystal structure of the tetrameric mouse dCTPase 1, PDB entry 2OIG. The cytosines of substrates 5-methyl-dCTP and dCTP bind deeply into the substrate pocket, which is composed of the residues from the dimer interface, including His38, Trp47, Glu63, Glu66, Trp73, Glu95, Asp98 and Tyr102 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.085 | - |
5-Methyl-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |  |
| 0.0944 | - |
5-iodo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.109 | - |
5-bromo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.152 | - |
dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.196 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.24 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.293 | - |
dATP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9H773 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| MCF-7 cell | - |
Homo sapiens | - |
| MDA-MB-231 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromo-dCTP + H2O | - |
Homo sapiens | 5-bromo-dCMP + diphosphate | - |
? | |
| 5-iodo-dCTP + H2O | - |
Homo sapiens | 5-iodo-dCMP + diphosphate | - |
? | |
| 5-methyl-dCTP + H2O | - |
Homo sapiens | 5-methyl-dCMP + diphosphate | - |
? | |
| dATP + H2O | - |
Homo sapiens | dAMP + diphosphate | - |
? | |
| dCTP + H2O | - |
Homo sapiens | dCMP + diphosphate | - |
? | |
| dTTP + H2O | - |
Homo sapiens | dTMP + diphosphate | - |
? | |
| dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
| additional information | no substrate: dGTP | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.081 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.108 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.14 | - |
dATP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.362 | - |
5-Methyl-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.367 | - |
dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.43 | - |
5-iodo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.505 | - |
5-bromo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 11 | more than 80% of maximum activity | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | knockdown of DCTPP1 in breast cancer cell line MCF-7 cells remarkably retards proliferation and colony formation in vitro. The capacity of mammosphere formation of MCF-7 is suppressed with the silence of DCTPP1, consistent with the enhanced mammosphere-forming ability in DCTPP1-overexpressing MDA-MB-231 cells. The global methylation level is elevated in DCTPP1-knockdown MCF-7 cells or mammosphere-forming MCF-7 cells but decreased significantly in DCTPP1-overexpressing MDA-MB-231 cells and its mammosphere | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.337 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.483 | - |
dATP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.551 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 2.4 | - |
dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 4.25 | - |
5-Methyl-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 4.56 | - |
5-iodo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 4.63 | - |
5-bromo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
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