Information on EC 3.4.23.17 - Pro-opiomelanocortin converting enzyme

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.23.17
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RECOMMENDED NAME
GeneOntology No.
Pro-opiomelanocortin converting enzyme
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage at paired basic residues in certain prohormones, either between them, or on the carboxyl side
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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aspartic endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
80891-34-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
a recombinant vaccinia virus vector is used to coexpress mouse prohormone convertase PC1 and PC2 in the constitutively secreting cell line BSC-40 and in the endocrine tissue-derived cell lines PC12 and AtT-20
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Manually annotated by BRENDA team
overexpressing BJ 3501 strain
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Manually annotated by BRENDA team
overexpressing BJ 3501 strain
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-lipotropin + H2O
?
show the reaction diagram
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-
-
-
?
NH2-terminal pro-opiomelanocortin1-77 + H2O
Lys-gamma3-melanotropin + NH2-terminal pro-opiomelanocortin1-49
show the reaction diagram
pro-enkephalin + H2O
?
show the reaction diagram
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-
-
-
?
Pro-insulin + H2O
Insulin A-chain + insulin B-chain
show the reaction diagram
pro-insulin + H2O
insulin chain A + insulin chain B
show the reaction diagram
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cleavage of a Lys-Arg-/- and Arg-Arg-/- bonds
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-
?
pro-neuropeptide Y + H2O
?
show the reaction diagram
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-
-
-
?
Pro-opiomelanocortin + H2O
?
show the reaction diagram
Pro-opiomelanocortin + H2O
Adrenocorticotropin + beta-lipotropin
show the reaction diagram
pro-opiomelanocortin + H2O
opiomelanocortin + pro-sequence of opiomelanocortin
show the reaction diagram
pro-vasopressin + H2O
neurophysin + glycopeptide
show the reaction diagram
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cleavage at a monobasic residue
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-
?
pro-vasopressin + H2O
vasopressin + pro-sequence of vasopressin
show the reaction diagram
-
cleavage of a Lys-Arg-/- bond
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-
?
Provasopressin + H2O
Arginine vasopressin-Gly10-Lys11-Arg12
show the reaction diagram
Small synthetic peptides with paired basic residues + H2O
?
show the reaction diagram
Human beta-lipotropin + H2O
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-lipotropin + H2O
?
show the reaction diagram
-
-
-
-
?
pro-enkephalin + H2O
?
show the reaction diagram
-
-
-
-
?
pro-insulin + H2O
insulin chain A + insulin chain B
show the reaction diagram
-
cleavage of a Lys-Arg-/- and Arg-Arg-/- bonds
-
-
?
pro-neuropeptide Y + H2O
?
show the reaction diagram
-
-
-
-
?
Pro-opiomelanocortin + H2O
?
show the reaction diagram
pro-opiomelanocortin + H2O
opiomelanocortin + pro-sequence of opiomelanocortin
show the reaction diagram
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hormone activation
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?
pro-vasopressin + H2O
neurophysin + glycopeptide
show the reaction diagram
-
cleavage at a monobasic residue
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-
?
pro-vasopressin + H2O
vasopressin + pro-sequence of vasopressin
show the reaction diagram
-
cleavage of a Lys-Arg-/- bond
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?
additional information
?
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the enzyme is highly specific for mono- and dibasic residues of prohormones, overview
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Diazoacetylnorleucine methyl ester
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Dithiodipyridine
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leupeptin
pepstatin A
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
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1 mM, stimulates 1.3fold
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0019 - 0.0025
beta-Lipotropin
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.5
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4 - 5
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4 - 5.5
5
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crude enzyme extract
additional information
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acidic pH optimum
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 4
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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intermediate lobe, anterior lobe, and neural lobe secretory granules
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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the enzyme is secreted
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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immunogenic cross-reactive with anti-yapsin 1 antibodies due to high structural homology
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 7.4, loss of activity 2-3 weeks after purification
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-20°C, purified enzyme, 2-3 days stable
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-20°C, stable for 2-3 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from pituitary gland to homogeneity
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overexpressing BJ 3501 strain
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a recombinant vaccinia virus vector is used to coexpress mouse prohormone convertase PC1 and PC2 in the constitutively secreting cell line BSC-40 and in the endocrine tissue-derived cell lines PC12 and AtT-20
-