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Literature summary for 3.4.23.17 extracted from
- Purification and characterization of a paired basic residue-specific pro-opiomelanocortin converting enzyme from bovine pituitary intermediate lobe secretory vesicles (1985), J. Biol. Chem., 260, 7194-7205.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiodipyridine | - |
Bos taurus |  |
| leupeptin | - |
Bos taurus | |
| additional information | not: diisopropyl fluorophosphate; phenylmethane sulfonylfluoride | Bos taurus | |
| pepstatin A | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, bovine | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Pro-opiomelanocortin + H2O | Bos taurus | may function as a pro-opiomelanocortin processing enzyme in the pituitary intermediate lobe | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypophysis | intermediate | Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.4, loss of activity 2-3 weeks after purification | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | poorly cleaved: benzyloxycarbonyl-Val-Lys-Lys-Arg 4-methoxy-2-naphthylamide | Bos taurus | ? | - |
? | |
| additional information | not: Lys-Lys-Arg-Arg residues in ACTH1-39 | Bos taurus | ? | - |
? | |
| Pro-insulin + H2O | - |
Bos taurus | Insulin A-chain + insulin B-chain | enzyme cleaves pro-insulin at the Lys-Arg pair to yield the A chain of insulin and at the Arg-Arg pair to yield the B-chain and the A-chain linked to C-chain (A-C) intermediate | ? | |
| Pro-opiomelanocortin + H2O | cleaves the peptide bonds either between the Lys and Arg or on the carboxyl side of the Arg at Lys-Arg pairs of pro-opiomelanocortin | Bos taurus | Adrenocorticotropin + beta-lipotropin | + beta-endorphin + MW 16000 NH2-terminal glycopeptide | ? | |
| Pro-opiomelanocortin + H2O | cleaves the peptide bonds either between the Lys and Arg or on the carboxyl side of the Arg at Lys-Arg pairs of pro-opiomelanocortin | Bos taurus | Adrenocorticotropin + beta-lipotropin | ACTH of MW 21000-23000, 13000 and 4500 | ? | |
| Pro-opiomelanocortin + H2O | may function as a pro-opiomelanocortin processing enzyme in the pituitary intermediate lobe | Bos taurus | ? | - |
? | |
| Small synthetic peptides with paired basic residues + H2O | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | SDS-PAGE | Bos taurus |
| ? | x * 70000, bovine | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | 3: about 60% of activity maximum | Bos taurus |
| 3 | 6 | 6: about 70% of activity maximum | Bos taurus |
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Release 2023.1 (January 2023)
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