Information on EC 3.2.1.11 - dextranase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.2.1.11
-
RECOMMENDED NAME
GeneOntology No.
dextranase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
SYSTEMATIC NAME
IUBMB Comments
6-alpha-D-glucan 6-glucanohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-70-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
aerobic bacterium SRI 2125
commentary isolated from the hot environs of a raw sugar factory
-
-
Manually annotated by BRENDA team
aerobic bacterium SRI 2126
commentary isolated from the hot environs of a raw sugar factory
-
-
Manually annotated by BRENDA team
aerobic bacterium SRI 2128
commentary isolated from the hot environs of a raw sugar factory
-
-
Manually annotated by BRENDA team
aerobic bacterium SRI 2140
commentary isolated from the hot environs of a raw sugar factory
-
-
Manually annotated by BRENDA team
anaerobic thermophilic bacterium AB11A
gram-negative, thermophilic, anaerobic, isolated from the non-volcanic low-temperature field, the Great Artesian Basin in Queensland
-
-
Manually annotated by BRENDA team
Arthrobacter oxydans
Arthrobacter oxydans DSM20119T
isolated from sugar-cane farm soil, gene aodex
UniProt
Manually annotated by BRENDA team
Arthrobacter oxydans KQ11
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain MT-G2
-
-
Manually annotated by BRENDA team
strain MT-G2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Catenovulum sp.
-
-
-
Manually annotated by BRENDA team
Catenovulum sp. SP03
-
-
-
Manually annotated by BRENDA team
Cellvibrio fulva
-
-
-
Manually annotated by BRENDA team
Chaetomium erraticum
-
-
-
Manually annotated by BRENDA team
var. coprophilum
-
-
Manually annotated by BRENDA team
var. coprophilum
-
-
Manually annotated by BRENDA team
var. coprophilum
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain M-73
-
-
Manually annotated by BRENDA team
dextranase II
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Lactobacillus bifidus
-
-
-
Manually annotated by BRENDA team
strain 1390
-
-
Manually annotated by BRENDA team
IGC 4047
-
-
Manually annotated by BRENDA team
KSM 22
-
-
Manually annotated by BRENDA team
NCYC 1436
-
-
Manually annotated by BRENDA team
no activity in Streptococcus anginosus
strain NCTC10713
-
-
Manually annotated by BRENDA team
no activity in Streptococcus anginosus NCTC10713
strain NCTC10713
-
-
Manually annotated by BRENDA team
no activity in Streptococcus constellatus
strain ATCC27823
-
-
Manually annotated by BRENDA team
no activity in Streptococcus constellatus ATCC27823
strain ATCC27823
-
-
Manually annotated by BRENDA team
no activity in Streptococcus gordonii
strain ATCC10558
-
-
Manually annotated by BRENDA team
no activity in Streptococcus gordonii ATCC10558
strain ATCC10558
-
-
Manually annotated by BRENDA team
no activity in Streptococcus intermedius
strain GAI-1157
-
-
Manually annotated by BRENDA team
no activity in Streptococcus intermedius GAI-1157
strain GAI-1157
-
-
Manually annotated by BRENDA team
no activity in Streptococcus oralis
strain ATCC10557
-
-
Manually annotated by BRENDA team
no activity in Streptococcus oralis ATCC10557
strain ATCC10557
-
-
Manually annotated by BRENDA team
no activity in Streptococcus sanguinis
strain ATCC10556
-
-
Manually annotated by BRENDA team
no activity in Streptococcus sanguinis ATCC10556
strain ATCC10556
-
-
Manually annotated by BRENDA team
strain Dex40-8 and Dex50-2
-
-
Manually annotated by BRENDA team
strain Dex70-1B and Dex70-34
-
-
Manually annotated by BRENDA team
Westling 1, I und II
-
-
Manually annotated by BRENDA team
strain D5884
-
-
Manually annotated by BRENDA team
Penicillium sp. NRRL 21969
strain NRRL 21969
-
-
Manually annotated by BRENDA team
Ig4a
-
-
Manually annotated by BRENDA team
Ig4a
-
-
Manually annotated by BRENDA team
strain UQM 733, dextranase D1, dextranase D2, dextranase D3 and dextranase D4
-
-
Manually annotated by BRENDA team
strain UQM 733, dextranase D1, dextranase D2, dextranase D3 and dextranase D4
-
-
Manually annotated by BRENDA team
strain TMO
UniProt
Manually annotated by BRENDA team
strain TMO
UniProt
Manually annotated by BRENDA team
strain 6R
-
-
Manually annotated by BRENDA team
NRRL 895
-
-
Manually annotated by BRENDA team
IP29-strain
-
-
Manually annotated by BRENDA team
strain ATCC19642, serotype a
SwissProt
Manually annotated by BRENDA team
strain E49, serotype a
SwissProt
Manually annotated by BRENDA team
strain HS-1, serotype a
-
-
Manually annotated by BRENDA team
strain MFe28, serotype h
SwissProt
Manually annotated by BRENDA team
strain MFe28, serotype h
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain K1-R
-
-
Manually annotated by BRENDA team
strain ATCC19645
-
-
Manually annotated by BRENDA team
strain BHT, serotype b
SwissProt
Manually annotated by BRENDA team
strain M36
-
-
Manually annotated by BRENDA team
strain M36
-
-
Manually annotated by BRENDA team
strain 6715, dextranase C and D
-
-
Manually annotated by BRENDA team
strain UAB66
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain NRRL 1768
-
-
Manually annotated by BRENDA team
ATCC9644
-
-
Manually annotated by BRENDA team
strain IMI068219
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain AB11Ad
-
-
Manually annotated by BRENDA team
thermophilic bacterium DP17
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester)-quenched dextran + H2O
?
show the reaction diagram
alternan + H2O
?
show the reaction diagram
amylopectin + H2O
?
show the reaction diagram
amylose + H2O
?
show the reaction diagram
arabinan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-fructan + H2O
?
show the reaction diagram
blue dextran + H2O
?
show the reaction diagram
blue dextran brain heart infusion agar plates
?
show the reaction diagram
blue-dextran + H2O
?
show the reaction diagram
dextran + H2O
?
show the reaction diagram
dextran + H2O
D-glucose + isomaltose
show the reaction diagram
-
-
-
-
?
dextran + H2O
D-glucose + isomaltose + isomalto-oligosaccharides
show the reaction diagram
-
-
-
-
?
dextran + H2O
d-glucose + isomaltose + isomaltotriose + ?
show the reaction diagram
-
-
-
-
?
dextran + H2O
D-glucose + isomaltotriose + isomaltose + isomaltooligosaccharide
show the reaction diagram
dextran + H2O
fragments of dextran
show the reaction diagram
dextran + H2O
isomalto-oligosaccharides
show the reaction diagram
dextran + H2O
isomalto-oligosaccharides + D-glucose
show the reaction diagram
dextran + H2O
isomaltooligosaccharides
show the reaction diagram
dextran + H2O
isomaltotetraose + cycloisomaltooligosaccharides
show the reaction diagram
-
main products are isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14
-
?
dextran + H2O
isomaltotriose
show the reaction diagram
dextran + H2O
isomaltotriose + D-glucose
show the reaction diagram
-
-
-
-
?
dextran + H2O
oligosaccharides
show the reaction diagram
dextran 742CB + H2O
?
show the reaction diagram
dextran derivates + H2O
?
show the reaction diagram
-
-
-
-
?
dextran T10 + H2O
isomaltose + isomaltotriose
show the reaction diagram
dextran T2000 + H2O
?
show the reaction diagram
dextran T2000 + H2O
isomaltose + isomaltotriose
show the reaction diagram
dextran T2000 + H2O
isomaltotriose + isomaltoteraose + isomaltopentaose
show the reaction diagram
-
-
main end-products
-
?
dextran T40 + H2O
isomaltose + isomaltotriose
show the reaction diagram
88% activity compared to dextran T70
-
-
?
dextran T500 + H2O
?
show the reaction diagram
dextran T500 + H2O
isomaltose + isomaltotriose
show the reaction diagram
96% activity compared to dextran T70
-
-
?
dextran T70 + H2O
isomaltose + isomaltotriose
show the reaction diagram
100% activity
-
-
?
dextran-100 + H2O
isomaltose
show the reaction diagram
dextrin + H2O
?
show the reaction diagram
-
-
-
-
?
glucan + H2O
?
show the reaction diagram
glucan + H2O
D-glucose + isomaltose + isomaltotriose + ?
show the reaction diagram
-
from Streptococcus sobrinus K1-R
-
-
?
glucan + H2O
fragments of glucan
show the reaction diagram
-
-
-
-
?
glycogen + H2O
?
show the reaction diagram
inulin + H2O
?
show the reaction diagram
isolmaltodextrins + H2O
?
show the reaction diagram
-
-
-
-
?
isomaltodextrin + H2O
?
show the reaction diagram
isomaltoheptaose + H2O
?
show the reaction diagram
isomaltohexaose + H2O
?
show the reaction diagram
isomaltopentaose + H2O
?
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
levan + H2O
?
show the reaction diagram
mutan + H2O
?
show the reaction diagram
nigeran + H2O
?
show the reaction diagram
pullulan + H2O
?
show the reaction diagram
Sephadex G-100 + H2O
?
show the reaction diagram
Sephadex G-150 + H2O
?
show the reaction diagram
-
-
-
-
?
Sephadex G-200 + H2O
?
show the reaction diagram
Sephadex G-25 + H2O
?
show the reaction diagram
Sephadex G-50 + H2O
?
show the reaction diagram
Sephadex G-75 + H2O
?
show the reaction diagram
soluble starch + H2O
?
show the reaction diagram
starch + H2O
?
show the reaction diagram
streptococcal glucan + H2O
?
show the reaction diagram
sucrose + H2O
?
show the reaction diagram
-
100% of the activity with dextran
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alternan + H2O
?
show the reaction diagram
dextran + H2O
fragments of dextran
show the reaction diagram
dextran + H2O
oligosaccharides
show the reaction diagram
dextran T10 + H2O
isomaltose + isomaltotriose
show the reaction diagram
dextran T2000 + H2O
isomaltose + isomaltotriose
show the reaction diagram
dextran T40 + H2O
isomaltose + isomaltotriose
show the reaction diagram
A8F6K5
88% activity compared to dextran T70
-
-
?
dextran T500 + H2O
isomaltose + isomaltotriose
show the reaction diagram
A8F6K5
96% activity compared to dextran T70
-
-
?
dextran T70 + H2O
isomaltose + isomaltotriose
show the reaction diagram
A8F6K5
100% activity
-
-
?
glucan + H2O
fragments of glucan
show the reaction diagram
-
-
-
-
?
pullulan + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AgNO3
-
1 mM, 71% activity compared to control without any metal ion
AlCl3
-
1 mM, 96% activity compared to control without any metal ion
BaCl2
-
1 mM, 90% activity compared to control without any metal ion
CaCl2
-
1 mM, 94% activity compared to control without any metal ion
CdCl2
-
1 mM, 87% activity compared to control without any metal ion
CoCl2
-
1 mM, 101% activity compared to control without any metal ion
CuCl2
-
1 mM, 107% activity compared to control without any metal ion
Fe2+
Arthrobacter oxydans
stimulates activity of the dextransucrase-dextranase fusion protein
Fe3+
-
no effect to enzyme stability
FeCl3
-
1 mM, 96% activity compared to control without any metal ion
HgCl2
-
1 mM, 73% activity compared to control without any metal ion
KCl
-
1 mM, 91% activity compared to control without any metal ion
Li+
Arthrobacter oxydans
stimulates activity of the dextransucrase-dextranase fusion protein
LiCl
-
1 mM, 91% activity compared to control without any metal ion
MgCl2
-
1 mM, 95% activity compared to control without any metal ion
MnCl2
-
1 mM, 71% activity compared to control without any metal ion
NaCl
-
1 mM, 94% activity compared to control without any metal ion
NiSO4
-
1 mM, 101% activity compared to control without any metal ion
RbCl
-
1 mM, 95% activity compared to control without any metal ion
SDS
-
1 mM, 94% activity compared to control without any metal ion
SnCl2
-
1 mM, 101% activity compared to control without any metal ion
ZnCl2
-
1 mM, 102% activity compared to control without any metal ion
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
4-chloromercuribenzoate
aflatoxin B1
inhibits dextranase activities of CYP3A4-expressing cultures, maximum inhibition of 22% at 2 microg/ml. Only negligibly inhibits strain INVSC1 expressing dextranase
aflatoxin G1
substantially less inhibitory towards dextranase activities of CYP3A4-expressing cultures than aflatoxin B1, showing a maximum inhibition of about 12% at 2 microg/ml, whereas strain INVSC1 expressing dextranase is only marginally inhibited
AgNO3
-
1 mM, 71% activity compared to control without any metal ion
Al3+
-
1 mM completely inhibits enzyme activity
AlCl3
-
1 mM, 96% activity compared to control without any metal ion
BaCl2
-
1 mM, 90% activity compared to control without any metal ion
CaCl2
-
1 mM, 94% activity compared to control without any metal ion
CdCl2
-
1 mM, 87% activity compared to control without any metal ion
deoxycholic acid
-
-
dextranase inhibitor
-
-
-
FeCl3
-
1 mM, 96% activity compared to control without any metal ion
Ferrous ammonium sulphate
-
-
guanidine/HCl
-
-
HgCl2
-
1 mM, 73% activity compared to control without any metal ion
iodoacetic acid
-
-
KCl
-
1 mM, 91% activity compared to control without any metal ion
Lead acetate
-
-
LiCl
-
1 mM, 91% activity compared to control without any metal ion
mercaptoethanol
-
immobilized enzyme
MgCl2
-
1 mM, 95% activity compared to control without any metal ion
MnCl2
-
1 mM, 71% activity compared to control without any metal ion
Na+
-
85% inhibition of the free enzyme, 60% inhibition of the immobilized enzyme
potassium ferricyanide
-
immobilized enzyme
RbCl
-
1 mM, 95% activity compared to control without any metal ion
Rose bengal
-
-
Sodium ascorbate
-
immobilized enzyme
Sodium azide
-
immobilized enzyme
sodium dodecylsulfate
Arthrobacter oxydans
-
0.05%, 14% residual activity
Sodium m-periodate
-
-
T-2 toxin
100% inhibition at 10 microg/ml of dextranase activities of CYP3A4-expressing cultures and strain INVSC1
Triton X-100
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
auxin
-
-
Ca2+
-
-
CoCl2
-
1 mM, 101% activity compared to control without any metal ion
CuCl2
-
1 mM, 107% activity compared to control without any metal ion
Mg2+
-
-
NiSO4
-
1 mM, 101% activity compared to control without any metal ion
SnCl2
-
1 mM, 101% activity compared to control without any metal ion
ZnCl2
-
1 mM, 102% activity compared to control without any metal ion
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00011 - 3.05
Dextran
0.0003 - 0.0004
Dextran T-2000
0.000000183 - 0.0095
dextran T2000
0.000288 - 2.1
isomaltoheptaose
0.000386 - 2.3
isomaltohexaose
0.0004 - 4.1
isomaltopentaose
4 - 6.5
isomaltotetraose
25 - 31
isomaltotriose
additional information
dextran 742CB
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
760 - 2230
Dextran
3.7
dextran 742CB
Thermoanaerobacter pseudethanolicus
-
pH 5.2, 70C
-
10.2 - 44.3
dextran T2000
8.1
dextran T500
Thermoanaerobacter pseudethanolicus
-
pH 5.2, 70C
-
7.93
isomaltoheptaose
Paenibacillus sp.
H2EI27
pH 5.5, 35C
7.53
isomaltohexaose
Paenibacillus sp.
H2EI27
pH 5.5, 35C
4.36
isomaltopentaose
Paenibacillus sp.
H2EI27
pH 5.5, 35C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
242000000
dextran T2000
Paenibacillus sp.
H2EI27
pH 5.5, 35C
78546
27500
isomaltoheptaose
Paenibacillus sp.
H2EI27
pH 5.5, 35C
4096
19500
isomaltohexaose
Paenibacillus sp.
H2EI27
pH 5.5, 35C
3244
10500
isomaltopentaose
Paenibacillus sp.
H2EI27
pH 5.5, 35C
3077
additional information
dextran 742CB
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.005 - 0.085
-
depends on the growth rate
0.03
aerobic bacterium SRI 2125
-
-
0.06
aerobic bacterium SRI 2128
-
-
0.12
anaerobic thermophilic bacterium AB11A
-
-
0.21
thermophilic bacterium DP17
-
-
0.4 - 40
-
units of dextran per ml, value depends on the medium
0.5
-
substrate alternan, pH 5.2, 70C
2.7
-
substrate dextran 742CB, pH 5.2, 70C
3.02
purified recombinant enzyme SmDex95, the 95 kDa variant, pH 5.0, temperature not specified in the publication
6.2
-
substrate dextran T500, pH 5.2, 70C
7.37
-
substrate dextran T2000, pH 5.2, 70C
8.21
purified recombinant enzyme SmDex90, the 90 kDa variant, pH 5.0, temperature not specified in the publication
11.9
-
pH 5.5, 45C
12.7
-
isomaltose
13.66
-
wild type enzyme, at pH 4.5, 30C
14.93
-
mutant enzyme D279C/S289C, at pH 4.5, 43C
18.2
-
dextranase II, U/ml
25.6
-
mutant enzyme T245C/N248C, at pH 5.0, 33C
34.6
-
dextranase I, U/ml
36.5
-
-
43
-
units/A units
44.52
-
mutant enzyme D279C/S289C, at pH 5.0, 33C
90
-
3.5% dextran; immobilized enzyme 57 U/mg
182
-
enzyme purified 17.56fold
188.3
-
enzyme purified 18.18fold
218
-
Tsuchiya medium
238
-
Fukumota medium
292
-
Fukumota medium
493
-
units/OD
762
-
-
770
-
dextranase I
950 - 1000
-
recombinant dextranase
1560
-
bovine serum albumin immobilized enzyme, pH 5.5, 60C
1670
-
dextranase II
1920
-
CD-II
2500
-
dextranase II
2560
-
dextranase I
8130
-
free enzyme, pH 5.5, 60C
10580
-
160000-molecular-weight dextranase
14500
-
175000-molecular-weight dextranase
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 5.5
-
dextranase D1
4.5 - 7.5
-
dextranase D2
4.8
-
dextranase I and II
5 - 6.5
-
-
5.4 - 5.8
Chaetomium erraticum
-
in juice, syrups are less optimal than juices
5.4
Chaetomium erraticum
-
soluble and adsorbed enzyme
5.4 - 5.8
-
in juice, syrups are less optimal than juices
5.6
-
immobilized enzyme
5.8
Arthrobacter oxydans
recombinant engineered fusion protein DSXR
6 - 6.5
-
dex2 from strain Dex50-2
6.2 - 6.7
-
-
7 - 7.5
-
dex1 from strain Dex40-8
7
-
dex1 from strain Dex50-2
7.5
Arthrobacter oxydans
-
-
8
Catenovulum sp.
-
-
additional information
-
optimum pH for activity 4.5-5.5 is not affected by immobilization
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 6
-
approx. 50% of maximal activity at pH 6.3, complete loss of activity above pH 7.5
3.5 - 7
-
less than 50% activity below pH 3.8 and above pH 6.2
4 - 5
-
-
4.5 - 8
-
activity range of both free and immobilized enzyme
4.5 - 11.8
-
4C and 24 h
5 - 6.4
5 - 5.5
5 - 6.5
5 - 9
-
approx. 50% of maximal activity at pH 5.2 and pH 8.0
5 - 7.5
-
at 37C for 60 min
5.5 - 8.3
Arthrobacter oxydans
70-100% activity at pH 6.0-8.0, 50% activity at pH 5.5 and pH 8.3
5.5 - 11
-
-
6 - 11
Catenovulum sp.
-
more than 60% of maximum activity
6.5 - 8
-
dex1 from strain Dex40-8, higher than 80% maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
Arthrobacter oxydans
-
35 - 40
-
dex1 from strain Dex40-8 and dex2 from strain Dex50-2,inactive above 50C
40
Catenovulum sp.
-
-
40 - 45
43.3 - 54.4
55 - 60
for 15 min incubation
58
-
dextranase D4
63
aerobic bacterium SRI 2125
-
-
additional information
-
ranges for both soluble or immobilized enzyme between 30-35C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
Catenovulum sp.
-
26.5% residual activity
25 - 60
Arthrobacter oxydans
over 80% activity retained within the range of 37-50C, 50% of maximal activity at 25C and 60C
25 - 45
-
approx. 50% of maximal activity at 30C, dramatic decrease in activity above 45C
28 - 40
-
marked decrease of activity above 40C and below 28C
30 - 60
-
approx. 35% of maximal activity at 30C, approx. 10% of maximal activity at 60C
35 - 50
Catenovulum sp.
-
more than 88% of maximum ativity
50 - 60
Chaetomium erraticum
-
non-concentrated dextranase 4
50 - 75
-
concentrated dextranase 1
55 - 60
55
-
35% activity
60
-
4% activity
65
-
0% activity
additional information
-
below 50C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
isoelectric focusing, isoenzyme 7
3.9
-
isoelectric focusing, isoenzyme 6
3.98
-
dextranase I
4.12
-
dextranase I
4.19
-
dextranase II
4.35
-
dextranase II
4.7
-
isoelectric focusing, isoenzyme 2
4.75
-
dextranase II
4.8
-
isoelectric focusing, isoenzyme 4
4.95
-
dextranase I
5.3
-
isoelectric focusing, isoenzyme 1
5.6
-
dextranase I
5.8
-
dextranase II
5.9
-
dextranase III
6.1
-
dextranase IV
6.9 - 7.3
-
dextranase D4
9.1
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
recombinant enzyme
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E)
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14000
-
electrophoretic analysis
39000
-
gel filtration
46000
-
ultracentrifugal analysis
50100
-
dextranase II
55800
-
dextranase I
65000
-
dextranase IV
66000
-
enzyme after deglycosylation, SDS-PAGE and Western blot analysis
67200
-
calculated from amino acid sequence
67600
-
calculated
68300
-
dex2 gene from strain Dex50-2, calculated from sequence analysis
68600
-
dex1 gene from Dex70-1B, calculated from sequence analysis
69200
-
dex1 gene from Dex70-34, calculated from sequence analysis
70000 - 120000
-
blue dextran in activity gels
70000
-
SDS-PAGE and Western blot analysis
73000
-
analyzed by SDS-PAGE, expressed in Pichia pastoris
74000
-
dextranase I
76000
-
dextranase I
78000
-
SDS-PAGE
80800
-
dex1 gene from strain Dex40-8, calculated from sequence analysis
89000
-
dextranase II
100000
100100
-
dex1 gene from strain Dex50-2, calculated from sequence analysis
110000
-
dextranase III
114000