Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.11 extracted from

  • Chen, L.; Zhou, X.; Fan, W.; Zhang, Y.
    Expression, purification and characterization of a recombinant Lipomyces starkey dextranase in Pichia pastoris (2008), Protein Expr. Purif., 58, 87-93.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into vector pPIC9k-His6 and expressed in Pichia pastoris GS115 strain under the control of AOX1 promoter Lipomyces starkeyi

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM completely inhibits enzyme activity Lipomyces starkeyi
Cu2+ 1 mM completely inhibits enzyme activity Lipomyces starkeyi
Fe3+ 1 mM completely inhibits enzyme activity Lipomyces starkeyi
SDS 1 mM completely inhibits enzyme activity Lipomyces starkeyi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM stimulates 2fold Lipomyces starkeyi
Co2+
-
Lipomyces starkeyi
K+
-
Lipomyces starkeyi
Mg2+ 1 mM enhances enzyme activity by 145% Lipomyces starkeyi
Mn2+
-
Lipomyces starkeyi
Na+
-
Lipomyces starkeyi
Ni2+
-
Lipomyces starkeyi
Zn2+
-
Lipomyces starkeyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
enzyme after deglycosylation, SDS-PAGE and Western blot analysis Lipomyces starkeyi
70000
-
SDS-PAGE and Western blot analysis Lipomyces starkeyi

Organism

Organism UniProt Comment Textmining
Lipomyces starkeyi
-
-
-
Lipomyces starkeyi 1390
-
-
-

Purification (Commentary)

Purification (Comment) Organism
17.56fold by cation-exchange chromatography, 18.18fold followed by Ni-affinity chromatography Lipomyces starkeyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.36
-
-
Lipomyces starkeyi
182
-
enzyme purified 17.56fold Lipomyces starkeyi
188.3
-
enzyme purified 18.18fold Lipomyces starkeyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
Lipomyces starkeyi ?
-
?
dextran + H2O
-
Lipomyces starkeyi 1390 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 70000, purified enzyme, SDS-PAGE and Western blot analysis Lipomyces starkeyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Lipomyces starkeyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Lipomyces starkeyi