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Literature summary for 3.2.1.11 extracted from
- Characterization of novel thermostable dextranase from Thermotoga lettingae TMO (2010), Appl. Microbiol. Biotechnol., 85, 581-587.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudothermotoga lettingae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 10% residual activity at 1 mM Ag+ | Pseudothermotoga lettingae |  |
| Fe2+ | 10% residual activity at1 mM Fe2+ | Pseudothermotoga lettingae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0095 | - |
dextran T2000 | in 40 mM sodium acetate buffer (pH4.3) at 55°C | Pseudothermotoga lettingae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity increases to 125% in the presence of 1 mM Mg2+ | Pseudothermotoga lettingae | |
| additional information | Ca2+, Li+, K+, and Na+ (with chloride) do not show appreciable effects on activity | Pseudothermotoga lettingae | |
| Ni2+ | activity increases to 125% in the presence of 1 mM Ni2+ | Pseudothermotoga lettingae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
SDS-PAGE | Pseudothermotoga lettingae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alternan + H2O | Pseudothermotoga lettingae | 15% activity compared to dextran T70 | ? | - |
? | |
| alternan + H2O | Pseudothermotoga lettingae TMO | 15% activity compared to dextran T70 | ? | - |
? | |
| dextran T10 + H2O | Pseudothermotoga lettingae | 73% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
| dextran T10 + H2O | Pseudothermotoga lettingae TMO | 73% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
| dextran T2000 + H2O | Pseudothermotoga lettingae | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | isomaltose + isomaltotriose | - |
? | |
| dextran T2000 + H2O | Pseudothermotoga lettingae TMO | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | isomaltose + isomaltotriose | - |
? | |
| dextran T40 + H2O | Pseudothermotoga lettingae | 88% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
| dextran T500 + H2O | Pseudothermotoga lettingae | 96% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
| dextran T70 + H2O | Pseudothermotoga lettingae | 100% activity | isomaltose + isomaltotriose | - |
? | |
| additional information | Pseudothermotoga lettingae | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | ? | - |
? | |
| additional information | Pseudothermotoga lettingae TMO | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | ? | - |
? | |
| pullulan + H2O | Pseudothermotoga lettingae | 4% activity compared to dextran T70 | ? | - |
? | |
| pullulan + H2O | Pseudothermotoga lettingae TMO | 4% activity compared to dextran T70 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudothermotoga lettingae | A8F6K5 | - |
- |
| Pseudothermotoga lettingae TMO | A8F6K5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alternan + H2O | 15% activity compared to dextran T70 | Pseudothermotoga lettingae | ? | - |
? | |
| alternan + H2O | 15% activity compared to dextran T70 | Pseudothermotoga lettingae TMO | ? | - |
? | |
| dextran T10 + H2O | 73% activity compared to dextran T70 | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
| dextran T10 + H2O | 73% activity compared to dextran T70 | Pseudothermotoga lettingae TMO | isomaltose + isomaltotriose | - |
? | |
| dextran T2000 + H2O | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
| dextran T2000 + H2O | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | Pseudothermotoga lettingae TMO | isomaltose + isomaltotriose | - |
? | |
| dextran T40 + H2O | 88% activity compared to dextran T70 | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
| dextran T500 + H2O | 96% activity compared to dextran T70 | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
| dextran T70 + H2O | 100% activity | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
| additional information | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | Pseudothermotoga lettingae | ? | - |
? | |
| additional information | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | Pseudothermotoga lettingae TMO | ? | - |
? | |
| pullulan + H2O | 4% activity compared to dextran T70 | Pseudothermotoga lettingae | ? | - |
? | |
| pullulan + H2O | 4% activity compared to dextran T70 | Pseudothermotoga lettingae TMO | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TLDex | recombinant protein | Pseudothermotoga lettingae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | for 15 min incubation | Pseudothermotoga lettingae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | - |
Pseudothermotoga lettingae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | over 75% of the original activity is sustained at 60°C for 4 h, the half-life of the enzyme is 1.5 h at 65°C with pH4.3 in the absence of added dextran | Pseudothermotoga lettingae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Pseudothermotoga lettingae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 4.5 | - |
Pseudothermotoga lettingae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 10 | stable from pH 4.3-10.0 after incubation at 4°C for 20 h, activity drops sharply above pH 10.0 and below pH 4.3 | Pseudothermotoga lettingae |
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