Information on EC 3.2.1.10 - oligo-1,6-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.10
-
RECOMMENDED NAME
GeneOntology No.
oligo-1,6-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by EC 3.2.1.1 (alpha-amylase), and in isomaltose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
-
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
-
-
Metabolic pathways
-
-
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
oligosaccharide 6-alpha-glucohydrolase
This enzyme, like EC 3.2.1.33 (amylo-alpha-1,6-glucosidase), can release an alpha-1->6-linked glucose, whereas the shortest chain that can be released by EC 3.2.1.41 (pullulanase), EC 3.2.1.142 (limit dextrinase), and EC 3.2.1.68 (isoamylase) is maltose. It also hydrolyses isomaltulose (palatinose), isomaltotriose and panose, but has no action on glycogen or phosphorylase limit dextrin. The enzyme from intestinal mucosa is a single polypeptide chain that also catalyses the reaction of EC 3.2.1.48 (sucrose alpha-glucosidase). Differs from EC 3.2.1.33 (amylo-alpha-1,6-glucosidase) in its preference for short-chain substrates and in its not requiring the 6-glucosylated residue to be at a branch point, i.e. linked at both C-1 and C-4.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-15-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain I42
-
-
Manually annotated by BRENDA team
strain I42
-
-
Manually annotated by BRENDA team
NY-14
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Bacillus sp. SAM1606
strain SAM1606
-
-
Manually annotated by BRENDA team
strain DSM 20083, gene aglA
SwissProt
Manually annotated by BRENDA team
strain UCC2003
UniProt
Manually annotated by BRENDA team
strain UCC2003
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Alaskan fur seal
-
-
Manually annotated by BRENDA team
GenPept accession number AAK28737.1
-
-
Manually annotated by BRENDA team
strain KP1006
-
-
Manually annotated by BRENDA team
barley
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Necturus sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Thermoanaerobium sp.
Tok6-B1
-
-
Manually annotated by BRENDA team
Thermoanaerobium sp. Tok6-B1
Tok6-B1
-
-
Manually annotated by BRENDA team
sea lion
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
sequence polymorphisms among the alpha-glucosidase family lead to interesting variability of gene expression patterns and of catalytic efficiencies on different substrates, which altogether account for the absence of functional redundancy for growth on isomaltose
metabolism
-
expression of the IMAx genes is regulated by carbon sources, putative binding sites of transcriptional regulators, overview. Ima1p isomaltase and Agt1p transporter are essential for isomaltose assimilation
additional information
catalytic residues are Glu277 and Asp352, isomaltase active site structure, role of the water chains in the active site pocket, the hydrogen bond network in the active site of isomaltase, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,6-di-O-alpha-D-glucitol + H2O
D-glucitol
show the reaction diagram
-
-
-
-
?
1,6-di-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
1,6-di-O-alpha-D-mannitol + H2O
D-mannitol
show the reaction diagram
-
-
-
-
?
1-O-alpha-D-glucopyranosyl-D-fructopyranose + H2O
D-fructose
show the reaction diagram
-
-
-
-
?
1-O-alpha-D-glucopyranosyl-D-glucitol + H2O
D-glucose + D-glucitol
show the reaction diagram
-
-
-
-
?
1-O-alpha-D-glucopyranosyl-D-mannitol + H2O
D-glucose + D-mannitol
show the reaction diagram
-
-
-
-
?
2'-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
2-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
3'-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
3-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
4'-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-glucoside + H2O
4-methylumbelliferol + alpha-D-glucose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 1-O-alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
show the reaction diagram
4-nitrophenyl alpha-D-glucopyranoside
4-nitrophenol + alpha-D-glucopyranose
show the reaction diagram
-
-
-
-
-
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
show the reaction diagram
4-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
6'-deoxy-methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
6(R)-ethyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
6(S)-ethyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
6-O-alpha-D-glucopyranosyl-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
6-O-alpha-D-glucopyranosyl-D-glucitol + H2O
D-glucose + D-glucitol
show the reaction diagram
-
-
-
-
?
6-O-alpha-D-glucopyranosyl-D-glucopyranose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-glucitol + H2O
D-glucose + D-glucitol
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol + H2O
D-glucose + D-mannitol
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-1)-D-mannitol + H2O
D-glucose + D-mannitol
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucitol + H2O
D-glucose + D-glucitol
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucitol + H2O
D-glucose + D-glucitol
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-6)-D-glucopyranose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
alpha-D-glucopyranosyl-(1-6)-beta-fructofuranosyl-alpha-D-glucopyranoside + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
alpha-limit dextrin + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-limit dextrins + H2O
D-glucose
show the reaction diagram
amylose + H2O
?
show the reaction diagram
-
n = 18, low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase
-
-
?
glycogen + H2O
?
show the reaction diagram
-
substrate from oyster, low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase
-
-
?
isomaltosaccharides + H2O
D-glucose
show the reaction diagram
isomaltose + H2O
2 D-glucose
show the reaction diagram
-
-
-
?
isomaltose + H2O
?
show the reaction diagram
isomaltose + H2O
alpha-D-glucose
show the reaction diagram
isomaltose + H2O
alpha-D-glucose + D-glucose
show the reaction diagram
isomaltose + H2O
D-glucose
show the reaction diagram
isomaltotriose + H2O
3 alpha-D-glucose
show the reaction diagram
isomaltriose + H2O
?
show the reaction diagram
kojibiose + H2O
D-glucose
show the reaction diagram
-
weak activity
-
?
maltodextrin + H2O
?
show the reaction diagram
-
corn maltodextrin
-
-
?
maltoheptaose + H2O
?
show the reaction diagram
-
low activity of mutant isomaltase
-
-
?
maltohexaose + H2O
?
show the reaction diagram
-
low activity of mutant isomaltase
-
-
?
maltopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
maltopentose + H2O
?
show the reaction diagram
-
-
-
-
?
maltose + H2O
D-glucose
show the reaction diagram
maltose + H2O
D-glucose + D-glucose
show the reaction diagram
-
-
-
-
?
maltotetraose + H2O
?
show the reaction diagram
maltotriitol + H2O
D-glucose + maltitol
show the reaction diagram
-
-
-
?
maltotriose + H2O
?
show the reaction diagram
maltotriose + H2O
D-glucose
show the reaction diagram
-
-
-
?
maltulose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
methyl alpha-D-glucopyranoside + H2O
methanol + alpha-D-glucopyranose
show the reaction diagram
-
-
-
?
methyl-alpha-isomaltoside + H2O
?
show the reaction diagram
-
-
-
-
?
nigerose + H2O
2 D-glucose
show the reaction diagram
nigerose + H2O
alpha-D-glucose + D-glucose
show the reaction diagram
oligosaccharide + H2O
monosaccharide
show the reaction diagram
p-nitrophenyl-alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
D-glucose + p-nitrophenol
show the reaction diagram
palatinose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
palatinose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
?
palatinose + H2O
D-glucose + fructose
show the reaction diagram
panitol
glucitol + maltitol
show the reaction diagram
-
-
-
?
panose + H2O
alpha-D-glucose
show the reaction diagram
panose + H2O
alpha-D-glucose + maltose
show the reaction diagram
preference for the cleavage of the alpha-1,6 linkage
-
-
?
panose + H2O
D-glucose + maltose
show the reaction diagram
phenyl-alpha-D-glucopyranoside + H2O
D-glucose + phenol
show the reaction diagram
pullulan + H2O
?
show the reaction diagram
-
low activity
-
-
?
pullulan + H2O
D-glucose + maltose
show the reaction diagram
-
-
-
?
soluble starch + H2O
?
show the reaction diagram
-
low activity of wild-type sucrase-isomaltase, very low activity of mutant isomaltase
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
trehalose + H2O
2 alpha-D-glucose
show the reaction diagram
-
-
-
?
trehalose + H2O
?
show the reaction diagram
trehalulose + H2O
alpha-D-glucose + beta-D-fructose
show the reaction diagram
-
-
-
?
turanose + H2O
alpha-D-glucose + beta-D-fructose
show the reaction diagram
-
-
-
?
turanose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
oligosaccharide + H2O
monosaccharide
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
Thermoanaerobium sp.
-
stabilization of activity by 1 mM at pH 5.6, 76C
Mg2+
Thermoanaerobium sp.
-
stabilization of activity by 1 mM at pH 5.6, 76C
Mn2+
Thermoanaerobium sp.
-
stabilization of activity by 1 mM at pH 5.6, 76C
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-1-azafagomine
-
i.e.(3R,4R,5R)-4,5-dihydroxy-3-hydroxymethylhexahydropyridazine, competitive, slow inhibition process, difference in Ki values depend almost entirely on changes in the binding rate constant, direct binding model, some analogues of the compound are also inhibitory with less efficiency
(2R,3S,4S)-1-[(2S,3S)-2,4-dihydroxy-3-(tridecyloxy)butyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
-
-
(2R,3S,4S)-1-[(2S,3S)-2,4-dihydroxy-3-methoxybutyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
-
-
(2R,3S,4S)-1-[(2S,3S)-3-(benzyloxy)-2,4-dihydroxybutyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
-
-
(2R,3S,4S)-1-[(2S,3S)-3-ethoxy-2,4-dihydroxybutyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
-
-
(2S,3S,4S,5S)-2-(hydroxymethyl)-5-[3-(4-methylphenyl)propyl]pyrrolidine-3,4-diol
-
-
(2S,3S,4S,5S)-2-(hydroxymethyl)-5-[4-(4-methoxyphenyl)butyl]pyrrolidine-3,4-diol
-
-
(2S,3S,4S,5S)-2-[4-(3,5-difluorophenyl)butyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
good inhibition activities against intestinal isomaltase and sucrase
1,2,3-tri-O-galloyl-beta-D-glucose
-
about 20% inhibition at 1 mM
1,4-anhydro-4-thio-D-arabinitol
-
-
1,4-dideoxy-1,4-((S)-[(2S,3S)-2,4-dihydroxy-3-butyl]episulfoniumylidene)-D-arabinitol
-
-
1,4-dideoxy-1,4-(hydroxyethyliminiumyl)-D-arabinitol
-
-
1,4-dideoxy-1,4-imino-D-arabinitol
-
-
1,4-dideoxy-1,4-imino-L-arabinitol
-
-
1,4-dideoxy-1,4-[(S)-[(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]episulfoniumylidene]-D-arabinitol
-
-
1,4-dideoxy-1,4-[(S)-[(2S,3S)-2,4-dihydroxy-3-butyl]episulfoniumylidene]-D-arabinitol
-
-
1,4-imino-1,2,4-trideoxy-D-arabinitol
-
-
1-O-Galloyl-beta-D-glucose
-
about 15% inhibition at 1 mM, 34.4% inhibition at 5 mM
1-O-methyl-2,3-di-O-galloyl-beta-D-glucose
-
about 10% inhibition at 1 mM
1-O-methyl-alpha-D-glucose
Thermoanaerobium sp.
-
42% inhibition by 62 mM
1-O-methyl-beta-D-glucose
Thermoanaerobium sp.
-
52% inhibition by 62 mM
2,5-dideoxy-2,5-imino-D-glucitol
-
-
2,5-dideoxy-2,5-imino-D-mannitol
-
-
2,5-dideoxy-2,5-imino-L-mannitol
-
-
2-Amino-2-ethyl-1,3-propanediol
-
80% inhibition of isomaltase activity by 50 mM
2-amino-2-methyl-1,3-propanediol
-
almost complete inhibition of isomaltase activity by 50 mM
3'-O-methylponkoranol
-
inhibits the different subunits to different extents, with extraordinary selectivity for C-terminal subunit of the enzyme
4-nitrophenyl alpha-D-glucopyranoside
-
acarbose
-
-
blintol
-
selenium analogue of salacinol
Ca2+
-
29% inhibition by 2 mM CaCl2
casuarictin
-
ellagtannin, from flower buds of Syzygium aromaticum, about 16% inhibition at 1 mM
Cd2+
-
complete inhibition
Chymotrypsin
-
almost 25% of palatinase activity after incubation with 0.1 mg/ml for 30 min
-
conduritol-B-epoxide
D-glucose
de-O-sulfonated kotalanol
de-O-sulfonated ponkoranol
-
-
de-O-sulfonated salacinol
-
-
diethanolamine
-
about 80% inhibition of isomaltase activity by 50 mM
DnaJ
-
the coexpression of the Hsp70 team chaperone protein leads to reduced activity
-
DnaK
-
the coexpression of the Hsp70 team chaperone protein leads to reduced activity
-
Elastase
-
almost 50% of palatinase activity after incubation with 0.1 mg/ml for 30 min
-
eugeniin
-
ellagtannin, from flower buds of Syzygium aromaticum, about 18% inhibition at 1 mM
Fe3+
-
86% inhibition by 2 mM FeCl3
gallic acid
-
8% inhibition at 5mM
glucono-delta-lactone
GrpE
-
the coexpression of the Hsp70 team chaperone protein leads to reduced activity
-
hepatocyte nuclear factor-1alpha
-
gene expression of HNF-1alpha exhibits a positive correlation with that of sucrase-isomaltase regulated by glucose
-
isofagomine
-
stereoisomer of (-)-1-azafagomine, racemic, competitive
KCl
-
40% inhibition by 1 M
kotalanol
maltose
methyl alpha-D-glucopyranoside
-
Mg2+
-
42% inhibition by 2 mM MgCl2
miglitol
-
-
Monoethanolamine
-
about 70% inhibition of isomaltase activity by 50 mM
monoiodoacetate
-
77% inhibition by 1 mM
mutant hepatocyte nuclear factor-1alpha
-
in the wild HNF-1alpha cells SI gene expression and enzyme activity is not significantly diminished
-
mutant hepatocyte nuclear factor-1beta
-
in the wild HNF-1beta cells SI gene expression and enzyme activity is not significantly diminished
-
N-ethylmaleimide
Thermoanaerobium sp.
-
70% inactivation by 3.3 mM at room temperature after 10 min
NaCl
-
50% inhibition by 500 mM
Ni2+
-
94% inhibition by 2 mM NiCl2
p-chloromercuribenzoate
p-nitrophenol
papain
penta-O-galloyl-beta-D-glucose
-
about 40% inhibition at 1-5 mM
Phenol
-
in combination with Tris most potent inhibitor
phenyl alpha-D-glucoside
Phenyl alpha-maltoside
-
92% inhibition by 10 mM, noncompetitive
pronase E
salacinol
SDS
Thermoanaerobium sp.
-
96% inhibition by 3.5 mM under standard conditions
Sn2+
-
complete inhibition
subtilisin BPN'
triethanolamine
-
about 80% inhibition of isomaltase activity by 50 mM
Trypsin
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Thermoanaerobium sp.
-
2.3fold increase of activity by 1 mM
dithiothreitol
Thermoanaerobium sp.
-
-
GroEL
-
heat shock protein of the Hsp60 team, GenBank accession number AB025944, activates 1.56fold, revives the thermally denatured enzyme, stabilizes and improve the recombinant expression level in Escherichia coli
-
GroES
-
heat shock protein of the Hsp60 team, GenBank accession number AB025944, activates 1.44fold, revives the thermally denatured enzyme, stabilizes and improve the recombinant expression level in Escherichia coli
-
additional information
-
gene expression and enzyme activity are changed by nutritional conditions
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012 - 13.3
4-nitrophenyl alpha-D-glucopyranoside
11 - 15
isomaltohexaose
4.6 - 21
isomaltopentaose
0.45 - 37
isomaltose
2.8 - 13
isomaltotetraose
1.7 - 16.7
isomaltotriose
72 - 128
isomaltriose
3.6
maltopentose
-
IP-SI, immunoprecipitated human sucrase-isomaltase complex
0.13 - 62.8
maltose
3.79
maltotetrose
-
IP-SI, immunoprecipitated human sucrase-isomaltase complex
4
maltotriitol
-
-
1.9 - 5.55
maltotriose
15 - 28
methyl alpha-D-glucopyranoside
0.17
p-nitrophenyl alpha-D-glucopyranoside
-
-
0.21 - 0.81
p-nitrophenyl-alpha-D-glucopyranoside
3.5 - 27.2
palatinose
1
Panitol
9.5 - 15
panose
2.2
phenyl-alpha-D-glucopyranoside
-
-
10.2 - 191
Sucrose
2 - 320
trehalose
22.9 - 30.2
Trehalulose
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 697
4-nitrophenyl alpha-D-glucopyranoside
15 - 103
isomaltose
12 - 12.5
isomaltotriose
15 - 21
isomaltriose
137
maltose
Homo sapiens
-
in 100 mM MES buffer, pH 6.5, at 37C
14 - 88
methyl alpha-D-glucopyranoside
6 - 104
palatinose
28.7 - 30
panose
3.8 - 55
Sucrose
5.8 - 7.3
trehalose
35.4 - 38.7
Trehalulose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13 - 28
4-nitrophenyl alpha-D-glucopyranoside
0.41 - 9
isomaltose
0.16 - 0.22
isomaltriose
19
maltose
Homo sapiens
-
in 100 mM MES buffer, pH 6.5, at 37C
86
0.9 - 3.1
methyl alpha-D-glucopyranoside
0.8 - 9.4
palatinose
0.02 - 0.38
Sucrose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00027
(-)-1-azafagomine
-
pH 6.8, 25C
0.000007 - 0.000035
3'-O-methylponkoranol
2 - 12
4-nitrophenyl alpha-D-glucopyranoside
0.014
acarbose
0.00016
blintol
-
N-terminal cataklytic domain, pH and temperature not specified in the publication
0.000012
de-O-sulfonated kotalanol
-
N-terminal cataklytic domain, pH and temperature not specified in the publication
0.000103 - 0.000302
de-O-sulfonated ponkoranol
0.0058
isofagomine
-
pH 6.8, 25C
188 - 248
isomaltose
0.0006
kotalanol
574 - 600
methyl alpha-D-glucopyranoside
0.000148
miglitol
-
N-terminal cataklytic domain, pH and temperature not specified in the publication
686 - 690
palatinose
0.000277
salacinol
-
N-terminal cataklytic domain, pH and temperature not specified in the publication
additional information
additional information
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.95
(2R,3S,4S)-1-[(2S,3S)-2,4-dihydroxy-3-(tridecyloxy)butyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
Homo sapiens
-
pH and temperature not specified in the publication
0.39
(2R,3S,4S)-1-[(2S,3S)-2,4-dihydroxy-3-methoxybutyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
Homo sapiens
-
pH and temperature not specified in the publication
0.14
(2R,3S,4S)-1-[(2S,3S)-3-(benzyloxy)-2,4-dihydroxybutyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
Homo sapiens
-
pH and temperature not specified in the publication
0.27
(2R,3S,4S)-1-[(2S,3S)-3-ethoxy-2,4-dihydroxybutyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
Homo sapiens
-
pH and temperature not specified in the publication
0.00063
(2S,3S,4S,5S)-2-(hydroxymethyl)-5-[3-(4-methylphenyl)propyl]pyrrolidine-3,4-diol
Rattus norvegicus
-
pH 6.8, 37C
0.0018
(2S,3S,4S,5S)-2-(hydroxymethyl)-5-[4-(4-methoxyphenyl)butyl]pyrrolidine-3,4-diol
Rattus norvegicus
-
pH 6.8, 37C
0.00022
(2S,3S,4S,5S)-2-[4-(3,5-difluorophenyl)butyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
Rattus norvegicus
-
pH 6.8, 37C
1
1,4-anhydro-4-thio-D-arabinitol
Rattus sp.
-
-
0.0057
1,4-dideoxy-1,4-((S)-[(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]episulfoniumylidene)-D-arabinitol
Rattus sp.
-
-
0.64
1,4-dideoxy-1,4-((S)-[(2S,3S)-2,4-dihydroxy-3-butyl]episulfoniumylidene)-D-arabinitol
Rattus sp.
-
-
0.07
1,4-dideoxy-1,4-(hydroxyethyliminiumyl)-D-arabinitol
Rattus sp.
-
-
0.0058
1,4-dideoxy-1,4-imino-D-arabinitol
Rattus sp.
-
-
0.00008
1,4-dideoxy-1,4-imino-L-arabinitol
Rattus sp.
-
-
0.73
1,4-imino-1,2,4-trideoxy-D-arabinitol
Rattus sp.
-
-
0.15
2,5-dideoxy-2,5-imino-D-glucitol
Rattus sp.
-
-
0.09
2,5-dideoxy-2,5-imino-D-mannitol
Rattus sp.
-
-
0.00005
2,5-dideoxy-2,5-imino-L-mannitol
Rattus sp.
-
-
0.3
de-O-sulfonated salacinol
Homo sapiens
-
pH and temperature not specified in the publication
1.3
salacinol
Homo sapiens
-
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3
-
mutant SIQ/P, 37C
0.4
maltulose; nigerose
1.02
-
purified enzyme, palatinose as substrate
1.2
substrate sucrose, pH 7.0, 30C
1.6
trehalose; turanose
2.12
-
purified mutant sucrase-free isomaltase, maltase activity
2.3
-
purified enzyme, isomaltose as substrate
2.6
-
mutant SIQ/P, 20C
2.8
-
wild-type enzyme, 20C
3.1
-
wild-type enzyme, 37C
3.9
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30C
4.5
-
with isomaltose as substrate
5
substrate palatinose, pH 7.0, 30C
5.1
-
purified recombinant enzyme + coexpressed DnaJ, DnaK, and GrpE
5.19
Thermoanaerobium sp.
-
purified enzyme, p-nitrophenyl-alpha-D-glucopyranoside as substrate
5.8
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30C
6.07
-
purified recombinant enzyme
7.2
substrate isomaltose, pH 7.0, 30C
7.6
substrate isomaltriose, pH 7.0, 30C
7.7
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30C
7.79
-
purified wild-type sucrase-isomaltase, sucrase activity
7.9
substrate isomaltriose, pH 7.0, 30C
8.08
-
purified wild-type sucrase-isomaltase, isomaltase activity
8.74
-
purified recombinant enzyme + coexpressed GroES
9.46
-
purified recombinant enzyme + coexpressed GroES and GroEL
9.48
-
purified recombinant enzyme + coexpressed GroEL
10.2
substrate sucrose, pH 7.0, 30C
11
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30C
12.3
-
purified mutant sucrase-free isomaltase, isomaltase activity
14.5
-
purified wild-type sucrase-isomaltase, maltase activity
18.6
substrate sucrose, pH 7.0, 30C
19.7
substrate sucrose, pH 7.0, 30C
34.2
-
purified enzyme, isomaltose as substrate
39
substrate palatinose, pH 7.0, 30C
52
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30C; substrate palatinose, pH 7.0, 30C
54
substrate isomaltose, pH 7.0, 30C
55
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30C
60
substrate isomaltose, pH 7.0, 30C
65
substrate isomaltose, pH 7.0, 30C
74
substrate palatinose, pH 7.0, 30C
218
-
1120fold purified enzyme
313
-
purified enzyme after expression in Escherichia coli, p-nitrophenyl-alpha-D-glucopyranoside as substrate
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.1 - 10.2
-
half-optima at pH 4.8 and 7.3
4.3 - 9.8
-
half-optima at pH 4.9 and 7.6
4.9 - 9.5
-
half-optima at pH 5.5 and 8.2
5.6 - 7
Thermoanaerobium sp.
-
-
5.7 - 8
-
half-optima at pH 5.75 and 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74
-
half-optima at 58C and 75C
additional information
-
mutant SIQ/P is temperature-sensitive with a permissive temperature for catalysis of 20C, at 37C intracellular degradation of the mutant protein occurs
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27 - 53
-
half-optima at 27C and 53C, no activity at 60C
47 - 75
-
half-optima at 47C and 75C, no activity at 78C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
primary and metastatic
Manually annotated by BRENDA team
Necturus sp.
-
airway
Manually annotated by BRENDA team
Necturus sp.
-
-
Manually annotated by BRENDA team
Necturus sp.
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000 - 33000
Thermoanaerobium sp.
-
SDS-PAGE, gel filtration
60000 - 62000
63000
-
Sepharyl S-200 gel filtration
68500
-
gel filtration
70000
SDS-PAGE; SDS-PAGE
100000
-
SDS-PAGE
102000
-
SDS-PAGE
130000
-
SDS-PAGE
140000 - 150000
-
SDS-PAGE, intact pancreatic duct, 2 polypeptide chains, 140000 + 150000
148000 <