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Literature summary for 3.2.1.10 extracted from
- Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis (1998), J. Bacteriol., 180, 2574-2578.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 40% inhibition by 1 M | Bacillus subtilis |  |
| NaCl | 50% inhibition by 500 mM | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
maltose | - |
Bacillus subtilis | |
| 0.21 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Bacillus subtilis | |
| 0.45 | - |
isomaltose | - |
Bacillus subtilis | |
| 10.2 | - |
sucrose | - |
Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oligosaccharide + H2O | Bacillus subtilis | direct function in maltose and isomaltose metabolism | monosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltosaccharides + H2O | n = 2, isomaltose | Bacillus subtilis | D-glucose | - |
? | |
| isomaltose + H2O | - |
Bacillus subtilis | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Bacillus subtilis | 2 D-glucose | - |
? | |
| oligosaccharide + H2O | direct function in maltose and isomaltose metabolism | Bacillus subtilis | monosaccharide | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus subtilis | D-glucose + p-nitrophenol | - |
? | |
| sucrose + H2O | - |
Bacillus subtilis | D-glucose + D-fructose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MalL | - |
Bacillus subtilis |
| oligo-1,4-1,6-alpha-glucosidase | - |
Bacillus subtilis |
| sucrase-isomaltase-maltase | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 8 | half-optima at pH 5.75 and 8.0 | Bacillus subtilis |
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