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EC Tree
The taxonomic range for the selected organisms is: Solanum lycopersicum The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
acid phosphatase, tartrate-resistant acid phosphatase, prostatic acid phosphatase, tracp, acpase, uteroferrin, tracp 5b, phosphatidic acid phosphatase, tracp5b, pp2a phosphatase,
more
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intracellular acid phosphatase
-
pi-starvation inducible purple acid phosphatase
-
secreted acid phosphatase
-
acid monophosphatase
-
-
-
-
acid nucleoside diphosphate phosphatase
-
-
-
-
Acid phosphatase PII
-
-
-
-
acid phosphohydrolase
-
-
-
-
acid phosphomoesterase
-
-
-
-
acid phosphomonoester hydrolase
-
-
-
-
Adipocyte acid phosphatase, isozyme alpha
-
-
-
-
Adipocyte acid phosphatase, isozyme beta
-
-
-
-
glycerophosphatase
-
-
-
-
intracellular acid phosphatase
Low molecular weight phosphotyrosine protein phosphatase
-
-
-
-
Minor phosphate-irrepressible acid phosphatase
-
-
-
-
pH 2.5 acid phosphatase
-
-
-
-
pH 6-optimum acid phosphatase
-
-
-
-
phosphomonoesterase
-
-
-
-
pi-starvation inducible purple acid phosphatase
-
secreted acid phosphatase
Stationary-phase survival protein surE
-
-
-
-
Tartrate-resistant acid ATPase
-
-
-
-
APase
-
-
-
-
IAP
-
-
intracellular acid phosphatase
-
-
intracellular acid phosphatase
-
PAP
-
-
-
-
purple acid phosphatase
-
-
purple acid phosphatase
-
SAP2
-
-
secreted acid phosphatase
-
-
secreted acid phosphatase
-
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phosphate-monoester phosphohydrolase (acid optimum)
Wide specificity. Also catalyses transphosphorylations.
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1-naphthyl phosphate + H2O
1-naphthol + phosphate
2-glycerophosphate + H2O
glycerol + phosphate
-
-
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
3-glycerate phosphate + H2O
glycerate + phosphate
-
-
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
6-phospho-D-gluconate + H2O
D-gluconate + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
-
-
-
?
bis-(p-nitrophenyl)phosphate + H2O
?
-
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
-
-
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
-
-
-
-
?
diphosphate + H2O
2 phosphate
Glucose 6-phosphate + H2O
Glucose + phosphate
-
-
-
-
?
glucuronate 6-phosphate + H2O
glucuronate + phosphate
-
-
-
-
?
glyceraldehyde 3-phosphate + H2O
glyceraldehyde + phosphate
-
-
-
-
?
glycerol 3-phosphate + H2O
glycerol + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
phenyl phosphate + H2O
phenol + phosphate
phosphoenolpyruvate + H2O
pyruvate + phosphate
phosphotyrosine + H2O
?
-
-
-
?
phosphotyrosine + H2O
tyrosine + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
pyridoxal 5'-phosphate + H2O
pyridoxal + phosphate
-
-
-
?
tetrapolyphosphate + H2O
?
-
reaction with SAP1 and SAP2
-
?
additional information
?
-
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
reaction with SAP1 and SAP2
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
-
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
reaction with SAP1 and SAP2
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
reaction with SAP1 and SAP2
-
?
ATP + H2O
?
-
-
-
?
ATP + H2O
?
-
reaction with SAP1 and SAP2
-
?
diphosphate + H2O
2 phosphate
-
-
-
?
diphosphate + H2O
2 phosphate
-
reaction with SAP2, no activity with SAP1
-
?
GTP + H2O
?
-
-
-
?
GTP + H2O
?
-
reaction with SAP1 and SAP2
-
?
phenyl phosphate + H2O
phenol + phosphate
-
-
-
?
phenyl phosphate + H2O
phenol + phosphate
-
reaction with SAP1 and SAP2
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
best substrate
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
reaction with SAP1 and SAP2
-
?
additional information
?
-
-
enzyme also exhibits significant peroxidase activity, which is optimal at pH 9.0 and insensitive to Mg2+ or molybdate
-
?
additional information
?
-
-
SAP1 and SAP2 are multifunctional enzymes, the enzymes also exhibit significant peroxidase activity which is optimal at approximately pH 8.4 and insensitive to Mg2+ or molybdate
-
?
additional information
?
-
-
the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism
-
-
?
additional information
?
-
-
substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits peroxidase activity, EC 1.11.1.7, optimal at pH 9.0 and insensitive to Mg2+ and molydate
-
-
?
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additional information
?
-
-
the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism
-
-
?
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Ba2+
-
5 mM, SAP1 is activated to 115% of control
EDTA
-
5 mM, SAP1 is activated to 114% of control
MgCl2
-
10 mM, 150% activated
MnCl2
-
10 mM, 130% activated
additional information
-
the enzyme is not affected by BaCl2, CaCl2, CoCl2, KCl, NaCl, or EDTA
Mg2+
-
5 mM, SAP1 is activated to 136% of control, SAP2 is activated to 181% of control
Mg2+
-
activates 150% at 10 mM
Mn2+
-
5 mM, SAP1 is activated to 113% of control
Mn2+
-
activates 130% at 10 mM
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Ba2+
-
5 mM, SAP2, 50% inhibition
Ca2+
-
5 mM, SAP2, 60% inhibition
Co2+
-
5 mM, SAP2, 95% inhibition
CuSO4
-
10 mM, 88% inhibition
EDTA
-
5 mM, SAP2, 71% inhibition
Fe3+
-
complete inhibition at 10 mM
FeCl3
-
10 mM, complete inhibition
Mn2+
-
5 mM, SAP2, 26% inhibition
ZnCl2
-
10 mM, 99% inhibition
Cu2+
-
99% inhibition at 10 mM
Cu2+
-
5 mM, SAP1, 91% inhibition
molybdate
-
-
phosphate
-
-
phosphate
-
mixed and competitive
vanadate
-
-
Zn2+
-
88% inhibition at 10 mM
Zn2+
-
5 mM, SAP2, complete inhibition of SAP1 and SAP2
additional information
-
SAP1 and SAP2 are not inhibited by tartrate
-
additional information
-
the purple enzyme is insensitive to L-tartrate
-
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additional information
-
phosphate starvation induces the enzyme activity 4fold
-
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3.4 - 6.4
1-naphthyl phosphate
0.18 - 4.5
2-naphthyl phosphate
6
3-phosphoglycerate
-
pH 5.3, 25°C, SAP2
3.3 - 4.5
4-nitrophenyl phosphate
4.5
6-phospho-D-gluconate
-
-
1.4
bis-(p-nitrophenyl)phosphate
-
pH 5.1, 25°C
5
glycerol 3-phosphate
-
pH 5.3, 25°C, SAP2
2.1 - 7.1
Phenyl phosphate
0.22 - 2.1
phosphoenolpyruvate
2.1 - 4.1
phosphotyrosine
2.7
pyridoxal 5'-phosphate
-
-
1.9 - 2.9
Tetrapolyphosphate
3.4
1-naphthyl phosphate
-
pH 5.1, 25°C
5.3
1-naphthyl phosphate
-
pH 5.3, 25°C, SAP2
6.4
1-naphthyl phosphate
-
pH 5.3, 25°C, SAP1
0.18
2-naphthyl phosphate
-
pH 5.1, 25°C
3.9
2-naphthyl phosphate
-
pH 5.3, 25°C, SAP1
4.5
2-naphthyl phosphate
-
pH 5.3, 25°C, SAP2
3.3
4-nitrophenyl phosphate
-
pH 5.3, 25°C, SAP2
4.5
4-nitrophenyl phosphate
-
pH 5.3, 25°C, SAP1
0.5
ATP
-
pH 5.1, 25°C
4.1
ATP
-
pH 5.3, 25°C, SAP1
5.5
ATP
-
pH 5.3, 25°C, SAP2
3.4
diphosphate
-
pH 5.1, 25°C
4.6
diphosphate
-
pH 5.3, 25°C, SAP2
0.16
GTP
-
pH 5.1, 25°C
5.4
GTP
-
pH 5.3, 25°C, SAP2
8.6
GTP
-
pH 5.3, 25°C, SAP1
2.1
Phenyl phosphate
-
pH 5.3, 25°C, SAP1
5.3
Phenyl phosphate
-
pH 5.3, 25°C, SAP2
7.1
Phenyl phosphate
-
pH 5.1, 25°C
0.22
phosphoenolpyruvate
-
pH 5.1, 25°C
1.4
phosphoenolpyruvate
-
pH 5.3, 25°C, SAP2
2.1
phosphoenolpyruvate
-
pH 5.3, 25°C, SAP1
2.1
phosphotyrosine
-
pH 5.3, 25°C, SAP2
4.1
phosphotyrosine
-
pH 5.1, 25°C
1.9
Tetrapolyphosphate
-
pH 5.3, 25°C, SAP1
2.9
Tetrapolyphosphate
-
pH 5.3, 25°C, SAP2
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0.0002 - 0.0005
molybdate
0.094
F-
-
pH 5.1
0.48
F-
-
pH 5.3, 25°C, SAP1
0.95
F-
-
pH 5.3, 25°C, SAP1
0.0002
molybdate
-
pH 5.1
0.0002
molybdate
-
pH 5.3, 25°C, SAP1 or SAP2
0.0004
molybdate
-
pH 5.7
0.0005
molybdate
-
pH 6.2
0.14
phosphate
-
pH 5.7
1.2
phosphate
-
pH 5.3, 25°C, SAP1
1.3
phosphate
-
pH 5.3, 25°C, SAP1
0.0065
vanadate
-
pH 5.1
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620
-
-
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3.5 - 4.5
-
isoenzyme 1, hydrolysis of p-nitrophenyl phosphate
5.3
-
isoenzyme SAP1 and SAP2
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2.6 - 6.3
-
pH 2.6: about 50% of maximal activity, pH 6.3: about 65% of maximal activity, isoenzyme 1, hydrolysis of p-nitrophenyl phosphate
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SAP1; cultivar Moneymaker
SwissProt
brenda
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-
brenda
-
brenda
-
brenda
-
brenda
-
phosphate-starved
brenda
-
-
brenda
-
brenda
-
brenda
-
brenda
-
brenda
-
brenda
-
brenda
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-
-
-
brenda
-
-
brenda
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PPH1_SOLLC
9
0
1005
Swiss-Prot
other Location (Reliability: 2 )
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120000
isozyme IAP, SDS-PAGE
57000
1 * 63000 + 1 * 57000, isozyme IAP, SDS-PAGE
63000
1 * 63000 + 1 * 57000, isozyme IAP, SDS-PAGE
84000
isozyme SAP1, SDS-PAGE
31000
-
2 * 31000, isoenzyme 1, SDS-PAGE
51000
-
isoenzyme 1, gel filtration
142000
-
native PAGE
142000
-
non-denaturing PAGE, gel filtration
57000
SAP2, SDS-PAGE
57000
-
alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE
57000
-
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE
63000
-
alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE
63000
-
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE
63000
1 * 63000 + 1 * 57000, isozyme IAP, SDS-PAGE
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heterodimer
1 * 63000 + 1 * 57000, isozyme IAP, SDS-PAGE
monomer
1 * 84000, isozyme SAP1, SDS-PAGE
heterodimer
1 * 63000 + 1 * 57000, isozyme IAP, SDS-PAGE
monomer
1 * 57000, isozyme SAP2, SDS-PAGE
additional information
-
peptide mapping
?
x * 50000, PAP1 exists as 50000 and 75000 isoforms, SDS-PAGE
?
x * 50700, PAP1, calculated from amino acid sequence
?
x * 75000, PAP1 exists as 50000 and 75000 isoforms, SDS-PAGE
dimer
-
2 * 31000, isoenzyme 1, SDS-PAGE
dimer
-
alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE
dimer
-
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE
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glycoprotein
-
-
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50
-
5 min, 20% loss of activity
50
-
loss of 20% activity after 5 min
65
-
5 min, 50% loss of activity
65
-
loss of 50% activity after 5 min
70
-
5 min, 80% loss of activity
70
-
loss of 80% activity after 5 min
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-80°C, purified enzyme is stable for at least 4 months
-
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intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography
-
two secreted acid phosphatases: SAP1 and SAP2
-
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phosphate deficiency increases intracellular and secretory enzyme activity in a concentration-dependent manner. The 50000 Da isoform PAP1 is induced after 2 days of phosphate starvation and the 75000 Da isoform is induced after 16 days of phosphate starvation
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Paul, E.M.; Williamson, V.M.
Purification and properties of acid phosphatase-1 from a nematode resistant tomato cultivar
Plant Physiol.
84
399-403
1987
Solanum lycopersicum
brenda
Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
Eur. J. Biochem.
269
6278-6286
2002
Solanum lycopersicum
brenda
Schenk, G.; Korsinczky, M.L.J.; Hume, D.A.; Hamilton, S.; DeJersey, J.
Purple acid phosphatases from bacteria: similarities to mammalian and plant enzymes
Gene
255
419-424
2000
Solanum lycopersicum
brenda
Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
Biochem. J.
377
419-428
2004
Solanum lycopersicum
brenda
Bozzo, G.G.; Dunn, E.L.; Plaxton, W.C.
Differential synthesis of phosphate-starvation inducible purple acid phosphatase isozymes in tomato (Lycopersicon esculentum) suspension cells and seedlings
Plant Cell Environ.
29
303-313
2006
Solanum lycopersicum (P83379), Solanum lycopersicum (P83380), Solanum lycopersicum
brenda
Suen, P.K.; Zhang, S.; Sun, S.S.
Molecular characterization of a tomato purple acid phosphatase during seed germination and seedling growth under phosphate stress
Plant Cell Rep.
34
981-992
2015
Solanum lycopersicum (A0A0C5AJS1)
brenda