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Literature summary for 3.1.3.2 extracted from

  • Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
    Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures (2004), Biochem. J., 377, 419-428.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information phosphate starvation induces the enzyme activity 4fold Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 99% inhibition at 10 mM Solanum lycopersicum
Fe3+ complete inhibition at 10 mM Solanum lycopersicum
fluoride mixed type Solanum lycopersicum
molybdate competitive Solanum lycopersicum
additional information the purple enzyme is insensitive to L-tartrate Solanum lycopersicum
phosphate mixed type Solanum lycopersicum
vanadate mixed type Solanum lycopersicum
Zn2+ 88% inhibition at 10 mM Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Solanum lycopersicum 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates 150% at 10 mM Solanum lycopersicum
Mn2+ activates 130% at 10 mM Solanum lycopersicum
additional information the enzyme is not affected by BaCl2, CaCl2, CoCl2, KCl, NaCl, or EDTA Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
63000
-
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
142000
-
native PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Solanum lycopersicum

Purification (Commentary)

Purification (Comment) Organism
intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture phosphate-starved Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
620
-
purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Solanum lycopersicum 4-nitrophenol + phosphate
-
?
additional information the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism Solanum lycopersicum ?
-
?
additional information substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits peroxidase activity, EC 1.11.1.7, optimal at pH 9.0 and insensitive to Mg2+ and molydate Solanum lycopersicum ?
-
?
phosphoenolpyruvate + H2O best substrate Solanum lycopersicum pyruvate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
More peptide mapping Solanum lycopersicum

Synonyms

Synonyms Comment Organism
IAP
-
Solanum lycopersicum
intracellular acid phosphatase
-
Solanum lycopersicum
PAP
-
Solanum lycopersicum
purple acid phosphatase
-
Solanum lycopersicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
loss of 20% activity after 5 min Solanum lycopersicum
65
-
loss of 50% activity after 5 min Solanum lycopersicum
70
-
loss of 80% activity after 5 min Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
-
Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
4.8 6.5
-
Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
molybdate pH 5.1 Solanum lycopersicum
0.0065
-
vanadate pH 5.1 Solanum lycopersicum
0.094
-
fluoride pH 5.1 Solanum lycopersicum
1.6
-
phosphate pH 5.1 Solanum lycopersicum