EC Number |
---|
3.1.3.2 | - |
3.1.3.2 | (NH4)2SO4 precipitation, DEAE cellulose separation and gel filtration |
3.1.3.2 | 12.2fold of recombinant from Escherichia coli strain BL21(DE3) by cation exchange and nickel chelate affinity chromatography |
3.1.3.2 | 120.7fold from root nodules and leaves by ammonium sulfate fractionation, DEAE ion exchange chromatography, ultrafiltration, and gel filtration |
3.1.3.2 | 16.1fold by anion exchange chromatography, gel filtration, and ultrafiltration |
3.1.3.2 | 1871fold by ammonium sulfate precipitation, hydrophobic interaction and ion exchange chromatography, and gel filtration |
3.1.3.2 | 2 interconvertible enzyme forms, the acid enzyme form is probably a complex between and basic form and an oligoribonucleotide factor |
3.1.3.2 | 2100fold to homogeneity from seeds |
3.1.3.2 | 3 isozymes 75fold, 389fold, and 191fold from seed cotyledons to homogeneity by successive ammonoum sulfate fractionation, gel filtration, concanavalin A affinity chromatography, and cation- and anion exchange chromatography |
3.1.3.2 | 852fold to homogeneity from embryonic axes by DEAE ion exchange and concanavalin A affinity chromatography |