Information on EC 2.7.1.30 - glycerol kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.30
-
RECOMMENDED NAME
GeneOntology No.
glycerol kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + glycerol = ADP + sn-glycerol 3-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycerol degradation I
-
-
degradation of sugar alcohols
-
-
Glycerolipid metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:glycerol 3-phosphotransferase
Glycerone and L-glyceraldehyde can act as acceptors; UTP (and, in the case of the yeast enzyme, ITP and GTP) can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-66-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
animal
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
oat
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Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
queen bumblebee
-
-
Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
Candida mycoderma
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
pigeon
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-
Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
Escherichia coli C.Lin 43
strain C.Lin 43
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
halophilic bacterium
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
-
-
-
Manually annotated by BRENDA team
Mycobacterium butyricum
-
-
-
Manually annotated by BRENDA team
607
-
-
Manually annotated by BRENDA team
607
-
-
Manually annotated by BRENDA team
-
-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
kidney bean
-
-
Manually annotated by BRENDA team
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-
-
Automatic Mining of ENzyme DAta
pea
-
-
Manually annotated by BRENDA team
plant
-
-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
-
-
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Automatic Mining of ENzyme DAta
-
-
-
Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
toad
-
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Uniprot
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
broad bean
-
-
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
the enzyme plays an essential role in central and lipid metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + sn-glycerol 3-phosphate
ATP + glycerol
show the reaction diagram
ATP + 1,3-propanediol
ADP + ?
show the reaction diagram
Candida mycoderma
-
weak
-
-
?
ATP + 1-deoxy-sn-glycerol
ADP + ?
show the reaction diagram
Candida mycoderma
-
-
-
-
?
ATP + 2-deoxyglycerol
ADP + ?
show the reaction diagram
Candida mycoderma
-
-
-
-
?
ATP + 2-mercaptoethanol
ADP + ?
show the reaction diagram
Candida mycoderma
-
-
-
-
?
ATP + 2-methylglycerol
ADP + ?
show the reaction diagram
Candida mycoderma
-
-
-
-
?
ATP + aminopropanediol
ADP + ?
show the reaction diagram
Candida mycoderma
-
R- and S-
-
-
?
ATP + D-glyceraldehyde
ADP + D-glyceraldehyde 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + dihydroxyacetone
ADP + dihydroxyacetone phosphate
show the reaction diagram
ATP + dihydroxypropyl dichloroacetate
ADP + ?
show the reaction diagram
-
glycerol analogue
-
-
?
ATP + glyceric acid
ADP + ?
show the reaction diagram
-
-
-
-
?
ATP + glycerol
?
show the reaction diagram
ATP + glycerol
ADP + sn-glycerol 3-phosphate
show the reaction diagram
ATP + L-glyceraldehyde
ADP + L-glyceraldehyde 3-phosphate
show the reaction diagram
ATP + mercaptopropanediol
1-mercaptopropanediol 1-phosphate + ADP
show the reaction diagram
ATP + monoacetin
ADP + ?
show the reaction diagram
-
glycerol analogue
-
-
?
ATP + monobutyrin
ADP + ?
show the reaction diagram
-
glycerol analogue
-
-
?
ATP + monothioglycerol
ADP + ?
show the reaction diagram
-
-
-
-
?
CTP + glycerol
CDP + glycerol 3-phosphate
show the reaction diagram
glycerol + ATP
sn-glycerol 3-phosphate + ADP
show the reaction diagram
GTP + glycerol
GDP + glycerol 3-phosphate
show the reaction diagram
ITP + glycerol
IDP + glycerol 3-phosphate
show the reaction diagram
TTP + glycerol
TDP + glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
UTP + glycerol
UDP + glycerol 3-phosphate
show the reaction diagram
XTP + glycerol
XDP + glycerol 3-phosphate
show the reaction diagram
Candida mycoderma
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + glycerol
?
show the reaction diagram
ATP + glycerol
ADP + sn-glycerol 3-phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
-
activates
Co2+
-
best effect, may be replaced by Zn2+
Zn2+
-
can substitute for Co2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-propanediol
-
-
1-Pentanol
-
-
3-Chloro-1,2-propanediol
-
-
3-Deoxy-sn-glycerol
Candida mycoderma
-
-
ATP
-
substrate inhibition
Butane-1,3-diol
Candida mycoderma
-
D- and L-configuration
CrATP
Candida mycoderma
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coordination complex of Cr3+ and ATP, dead-end inhibitor
cytosolic subunit of the glucose-specific phosphotransferase system
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allosteric inhibition
-
D-fructose 1,6-bisphosphate
-
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D-fructose-1,6-bisphosphate
-
allosteric inhibition
DTNB
-
inactivation reversed by dithiothreitol
erythritol
Candida mycoderma
-
-
ethanediol
ethanol
Candida mycoderma
-
-
fructose 1,6-bisphosphate
fructose 1,6-diphosphate
glucose
glucose 6-phosphate
glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system
-
-
-
glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc))
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allosteric inhibitor
-
glycerol
glycerol 3-phosphate
iodoacetamide
iodoacetate
-
-
L(+)-Butane-2,3-diol
Candida mycoderma
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-
L-alpha-glycerophosphate
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no inhibition up to 3 mM in presence of 0.1 M glycerol
L-Threitol
Candida mycoderma
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-
N-ethylmaleimide
p-chloromercuribenzoate
p-Hydroxymercuriphenylsulfonate
-
-
phosphocarrier protein IIAGlc
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the unphosphorylated form of the phosphocarrier protein IIAGlc is an allosteric inhibitor of Escherichia coli glycerol kinase
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Procion Blue MX-3G
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5 mM, inactivates after a period of increased activity
propan-1-ol
propan-2-ol
Salyrganic acid
Candida mycoderma
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,3-butanediol
-
activates
1,3-Propanediol
-
activates
1,4-Butanediol
-
activates
1-butanol
-
activates
1-Chloro-2-propanol
-
activates
1-propanol
2,3-Butanediol
-
activates
2-butanol
-
activates
2-Chloroethanol
-
activates
2-methyl-1-propanol
-
activates
2-Methyl-2-propanol
-
activates
2-Pentanol
-
activates
2-propanol
3-methyl-1-butanol
-
activates
Cyclohexanol
-
activates
Ethanediol methyl ether
-
activates
ethanol
-
activates
methanol
-
activates
rosiglitazone
-
rosiglitazone treatment markebly increases glycerol kinase mRNA expression in both the mesenteric and epididymal adipose tissues (1085.9% and 523.8% of the abundance in Long-Evans Tokushima Otsuka rats, respectively). The magnitude of glycerol kinase induced by rosiglitazone is significantly greater in the mesenteric fat than in the epididymal fat.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45
1-deoxy-sn-glycerol
Candida mycoderma
-
-
88
2-deoxyglycerol
Candida mycoderma
-
-
0.046
2-mercaptoethanol
Candida mycoderma
-
25C, pH 8.2
5.7
2-methylglycerol
Candida mycoderma
-
-
0.24 - 0.69
ADP
0.006 - 3.29
ATP
0.515
CTP
-
-
0.14
dichloro-monoacetin
-
pH 7.35, 37C
0.5 - 100
dihydroxyacetone
0.15
glyceric acid
0.01 - 2
glycerol
12.56
glycerol-3-phosphate
-
pH 7.0
0.145
GTP
-
-
3 - 42
L-Glyceraldehyde
3.83
L-glycerol-3-phosphate
-
25C, pH 8.0
0.009 - 0.01
MgATP2-
0.169
Monoacetin
-
pH 7.35, 37C
0.491
Monobutyrin
-
pH 7.35, 37C
4.9
monothioglycerol
1.09 - 1.37
sn-glycerol 3-phosphate
0.62
UTP
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1670
glycerol
Candida mycoderma
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250
propan-1-ol
Candida mycoderma
-
-
1500
propan-2-ol
Candida mycoderma
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.01
glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc))
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.054
-
recombinant enzyme, using 1% methanol as carbon sources in the medium, at pH 9.8 and 30C
0.13
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recombinant enzyme, using 1% glycerol and 1% methanol as carbon sources in the medium, at pH 9.8 and 30C
2
-
at pH 7.0 and 70C
41.2
-
pH 7.0, 25C
101
Candida mycoderma
-
25C
177
-
pH 7.5, 25C
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 10.5
-
pH 6.8: about 30% of maximum activity, pH 10.5: about 80% of maximum activity
7 - 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 80
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30C: about 50% of maximum activity, 80C: about 65% of maximum activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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lung cell line
Manually annotated by BRENDA team
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SS6-2 cell, stem cells from fat with myotube characteristics
Manually annotated by BRENDA team
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newborn, expresses only isoform GK-EX18
Manually annotated by BRENDA team
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from patients with enzyme deficiency and normal individuals
Manually annotated by BRENDA team
-
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Automatic Mining of ENzyme DAta
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kidney cell line
Manually annotated by BRENDA team
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kidney cell line, expresses only isoform GK+EX18
Manually annotated by BRENDA team
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adult hepatoblastoma cell line
Manually annotated by BRENDA team
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4th instar larvae
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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fetal liver cell line
Manually annotated by BRENDA team
additional information