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Literature summary for 2.7.1.30 extracted from

  • Bergmeyer, H.U.; Holz, G.; Kauder, E.M.; Möllering, H.; Wieland, O.
    Kristallisierte Glycerokinase aus Candida mycoderma (1961), Biochem. Z., 333, 471-480.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Candida mycoderma

General Stability

General Stability Organism
dialysis, against 0.01 M phosphate buffer, at 2°C, pH 6.7, 50% loss of activity without glycerol, stable in presence of 0.01 M glycerol Candida mycoderma

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Candida mycoderma
p-chloromercuribenzoate
-
Candida mycoderma
Salyrganic acid
-
Candida mycoderma

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
glycerol 25°C Candida mycoderma
0.09
-
ATP 25°C Candida mycoderma

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
251000
-
diffusion and sedimentation data Candida mycoderma

Organism

Organism UniProt Comment Textmining
Candida mycoderma
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida mycoderma

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Candida mycoderma
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101
-
25°C Candida mycoderma

Storage Stability

Storage Stability Organism
4°C, crystallized suspension in 2.2 M ammonium sulfate, stable for several months Candida mycoderma

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dihydroxyacetone
-
Candida mycoderma ADP + dihydroxyacetone phosphate
-
?
ATP + glycerol
-
Candida mycoderma ADP + sn-glycerol 3-phosphate
-
?
GTP + glycerol
-
Candida mycoderma GDP + glycerol 3-phosphate
-
?
ITP + glycerol
-
Candida mycoderma IDP + glycerol 3-phosphate
-
?
UTP + glycerol
-
Candida mycoderma UDP + glycerol 3-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Candida mycoderma

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1670
-
glycerol
-
Candida mycoderma

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.7
-
highest stability Candida mycoderma