Literature summary for 2.7.1.30 extracted from

Katsumi, R.; Koga, Y.; You, D.J.; Matsumura, H.; Takano, K.; Kanaya, S.
Crystallization and preliminary x-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis (2007), Acta Crystallogr. Sect. F, F63, 126-129.

Cloned(Commentary)

Cloned (Comment)	Organism
overproduced in Escherichia coli BL21 (DE3)	Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
using sitting-drop vapour-diffusion method at 293 K. Native Tk-glycerol kinase crystals appear after a few days using Wizard I solution No. 25 (0.1 M Tris pH 8.5, 0.2 M MgCl2, 30% PEG 400). Diffraction spots sufficient for structural determination at high resolution are not obtained when a crystal of Tk-glycerol kinase is mounted on a CryoLoop without cryoprotectant, diffraction patterns of the crystal are improved by using Paratone-N as cryoprotectant. Native X-ray diffraction data are collected to 2.4 A resolution using synchrotron radiation at station BL44XU of SPring-8. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = b = 217.48, c = 66.48 A. Assuming the presence of two molecules in the asymmetric unit, the VM value is 2.7 A3Da-1 and the solvent content is 54.1%.	Thermococcus kodakarensis

Crystallization (Comment)

Organism

using sitting-drop vapour-diffusion method at 293 K. Native Tk-glycerol kinase crystals appear after a few days using Wizard I solution No. 25 (0.1 M Tris pH 8.5, 0.2 M MgCl2, 30% PEG 400). Diffraction spots sufficient for structural determination at high resolution are not obtained when a crystal of Tk-glycerol kinase is mounted on a CryoLoop without cryoprotectant, diffraction patterns of the crystal are improved by using Paratone-N as cryoprotectant. Native X-ray diffraction data are collected to 2.4 A resolution using synchrotron radiation at station BL44XU of SPring-8. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = b = 217.48, c = 66.48 A. Assuming the presence of two molecules in the asymmetric unit, the VM value is 2.7 A3Da-1 and the solvent content is 54.1%.

Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	SDS-PAGE	Thermococcus kodakarensis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	-	hyperthermophilic archaeon	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrapQ HP column, Bio-Scale CHT20-I column, all purification procedures are carried out at 277 K.	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
GK	glycerol kinase	Thermococcus kodakarensis