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Literature summary for 2.7.1.30 extracted from

  • Applebee, M.K.; Joyce, A.R.; Conrad, T.M.; Pettigrew, D.W.; Palsson, B.O.
    Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli (2011), J. Biol. Chem., 286, 23150-23159.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Shot BL21 star (DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D72V the catalytic properties of the mutant differ little from those of the wild type enzyme. The mutant shows 14.76% expression compared to the wild type enzyme Escherichia coli
M271I the mutant shows strongly increased Km for ATP and 30.75% expression compared to the wild type enzyme Escherichia coli
Q37P the mutant shows strongly increased Km for ATP and 65.73% expression compared to the wild type enzyme Escherichia coli
V61L the catalytic properties of the mutant differ little from those of the wild type enzyme. The mutant shows 12.71% expression compared to the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ATP substrate inhibition Escherichia coli
cytosolic subunit of the glucose-specific phosphotransferase system allosteric inhibition Escherichia coli
D-fructose-1,6-bisphosphate allosteric inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 ATP mutant enzyme V61L, at pH 7.0 and 25°C Escherichia coli
0.0078
-
 ATP wild type enzyme, at pH 7.0 and 25°C Escherichia coli
0.009
-
 ATP mutant enzyme D72V, at pH 7.0 and 25°C Escherichia coli
0.19
-
 ATP mutant enzyme Q37P, at pH 7.0 and 25°C Escherichia coli
0.25
-
 ATP mutant enzyme M271I, at pH 7.0 and 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+-ATP-dependent Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 or 4 * 60000, in solution, the enzyme exists in a dimer-tetramer equilibrium Escherichia coli
158000
-
 dimeric native enzyme, gel filtration Escherichia coli
224000
-
 tetrameric native enzyme, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 MG1655 (ATCC 47076)
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap FF column chromatography, gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol
-
 Escherichia coli ADP + sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer or tetramer 2 or 4 * 60000, in solution, the enzyme exists in a dimer-tetramer equilibrium Escherichia coli

Synonyms

Synonyms Comment Organism
GLPK
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP Mg2+-ATP-dependent Escherichia coli