Information on EC 2.7.1.113 - deoxyguanosine kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.113
-
RECOMMENDED NAME
GeneOntology No.
deoxyguanosine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + deoxyguanosine = ADP + dGMP
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
purine deoxyribonucleosides salvage
-
-
purine metabolism
-
-
Purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:deoxyguanosine 5'-phosphotransferase
Deoxyinosine can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
39471-28-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; gene yaaG
-
-
Manually annotated by BRENDA team
piglet
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
-
deoxyguanosine kinase is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. The main supply of purine dNTPs for mtDNA synthesis comes from the salvage pathway initiated by the enzyme
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxyguanosine
2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxyguanosine
2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
low activity
-
-
?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxyguanosine
2'-deoxyadenosine-3'-diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-2'-triphosphate + 2'-deoxyguanosine
3'-deoxyadenosine-2'-diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxyguanosine
3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxyguanosine
3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
ADP + ?
show the reaction diagram
-
4.4% of the activity with 2'-deoxyguanosine
-
-
?
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
ADP + ?
show the reaction diagram
-
poor substrate, 1.1% of the activity with 2'-deoxyguanosine
-
-
?
ATP + 1-beta-D-arabinofuranosyladenine
ADP + 1-beta-D-arabinofuranosyladenine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2',2'-difluorodeoxyguanosine
ADP + 2',2'-difluorodeoxyguanosine 5'-monophosphate
show the reaction diagram
recombinant mitochondrial deoxyguanosine kinase, 147% of activity with deoxyguanosine
-
-
ATP + 2',2'-difluorodeoxyguanosine
ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2',3'-dideoxyguanosine
ADP + 2',3'-dideoxyguanosine 5'-monophosphate
show the reaction diagram
-
recombinant deoxyguanosine kinase, 14% of activity with deoxyguanosine
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
show the reaction diagram
ATP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
ADP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine 5'-monophosphate
show the reaction diagram
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine
ADP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
show the reaction diagram
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-monophosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2-fluoro-arabinosyl-adenine
ADP + 2-fluoro-arabinosyl-adenine 5'-monophosphate
show the reaction diagram
-
recombinant mitochondrial deoxyguanosine kinase
-
?
ATP + 2-fluoroadenine-beta-D-arabinofuranoside
ADP + 2-fluoroadenine-beta-D-arabinofuranoside 5'-monophosphate
show the reaction diagram
-
-
-
-
?
ATP + 3'-fluoro-2',3'-dideoxyguanosine
ADP + 3'-fluoro-2',3'-dideoxyguanosine 5'-monophosphate
show the reaction diagram
-
recombinant deoxyguanosine kinase, 14% of activity with deoxyguanosine
-
?
ATP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine
ADP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5'-monophosphate
show the reaction diagram
-
antiherpesvirus drug ganciclovir, recombinant mitochondrial deoxyguanosine kinase, 6% of activity with deoxyguanosine
-
?
ATP + 9-(4-hydroxy-3-hydroxymethylbutyl-1-yl)guanine
ADP + 9-(4-hydroxy-3-hydroxymethylbutyl-1-yl)guanine 5'-monophosphate
show the reaction diagram
-
antiherpesvirus drug penciclovir, recombinant mitochondrial deoxyguanosine kinase, 50% of activity with deoxyguanosine
-
?
ATP + 9-beta-D-arabinofuranosylguanine
ADP + 9-beta-D-arabinofuranosylguanine 5'-monophosphate
show the reaction diagram
ATP + 9-beta-D-arabinofuranosylguanine
ADP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 9-beta-D-arabinosylguanine
ADP + 9-beta-D-arabinosylguanine 5'-monophosphate
show the reaction diagram
-
-
-
?
ATP + arabinosyl adenine
ADP + arabinosyl adenine 5'-monophosphate
show the reaction diagram
-
recombinant mitochondrial deoxyguanosine kinase, very low activity
-
?
ATP + arabinosyl guanine
ADP + arabinosyl guanine 5'-monophosphate
show the reaction diagram
-
recombinant mitochondrial deoxyguanosine kinase, better substrate than deoxyguanosine kinase
-
?
ATP + cytarabine
ADP + cytarabine 5'-monophosphate
show the reaction diagram
-
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
ATP + deoxyinosine
ADP + dIMP
show the reaction diagram
ATP + dideoxyinosine
ADP + dideoxyinosine 5'-monophosphate
show the reaction diagram
-
recombinant mitochondrial deoxyguanosine kinase, very low activity
-
?
ATP + dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
show the reaction diagram
ATP + ganciclovir
ADP + ganciclovir phosphate
show the reaction diagram
i.e. 9-(1,3-dihydroxy-2-propoxymethyl)-guanine, low activity
-
-
?
ATP + guanosine
ADP + GMP
show the reaction diagram
ATP + penciclovir
ADP + penciclovir 5'-phosphate
show the reaction diagram
i.e. 9-(2-hydroxy-1-hydroxymethyl-ethoxymethyl)-guanine, low activity
-
-
?
CTP + 2'-deoxyguanosine
CDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
CTP + deoxyguanosine
CDP + dGMP
show the reaction diagram
dCTP + deoxyguanosine
dCDP + dGMP
show the reaction diagram
dTTP + deoxyguanosine
dTDP + dGMP
show the reaction diagram
dUTP + deoxyguanosine
dUDP + dGMP
show the reaction diagram
-
69% of activity with ATP at pH 6.0, 1.7fold higher activity than with ATP at pH 7.0
-
?
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
very low activity
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
very low activity
-
-
?
GTP + 2'-deoxyguanosine
GDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
GTP + 2'-deoxyinosine
GDP + 2'-deoxyinosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
GTP + deoxyguanosine
GDP + dGMP
show the reaction diagram
-
50% of activity with ATP
-
?
GTP + guanosine
GDP + guanosine 5'-phosphate
show the reaction diagram
-
very low activity
-
-
?
triphosphate + 2'-deoxyguanosine
diphosphate + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
triphosphate is active with 2'deoxyguanosine, 50% of the activity with ATP
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
UTP + 2'-deoxyinosine
UDP + 2'-deoxyinosine 5'-phosphate
show the reaction diagram
UTP + 9-beta-D-arabinofuranosylguanine
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
show the reaction diagram
-
i.e. nelarabine, activation of the anticancer drug
-
-
?
UTP + deoxyguanosine
UDP + dGMP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
show the reaction diagram
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
ATP + deoxyinosine
ADP + dIMP
show the reaction diagram
-
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
-
?
ATP + dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
show the reaction diagram
-
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
Q16854
-
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
Q16854
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-deoxyadenosine-3'-fluoro-3'-triphosphate
-
70% of the activity with ATP
-
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate
-
13% of the activity with ATP
-
2'-deoxyadenosine-3'-triphosphate
-
53% of the activity with ATP
-
3'-deoxyadenosine-2'-triphosphate
-
119% of the activity with ATP
-
3'-deoxyadenosine-3'-fluoro-2'-triphosphate
-
25% of the activity with ATP
-
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate
-
55% of the activity with ATP
-
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
slight activation
Cr2+
-
slight activation
Cu2+
-
25% of activity with Mg2+
Ni2+
-
slight activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-Cyclohexanedione
-
-
1-alpha-D-arabinofuranosylguanine
-
-
2,3-Butanedione
-
-
2-fluoro-9-beta-D-arabinofuranosyladenine
-
-
8-aza-2'-deoxyguanosine
-
-
8-azadeoxyguanosine
-
-
9-beta-D-arabinofuranosylguanine
-
-
AMP
-
weak inhibition
arabinoadenosine
-
-
arabinocytidine
-
-
arabinosylguanine
-
-
ATP
-
weak inhibition
carbodiimide
-
-
cladribine
-
-
clofarabine
-
-
CMP
-
weak inhibition
CuSO4
-
-
cytarabine
-
-
dADP
-
1 mM, 49% inhibition at pH 6.0
dAMP
-
-
dCDP
-
-
dCMP
-
weak inhibition
deoxyadenosine
deoxyinosine
diphosphate
-
competitive inhibition
dTMP
-
weak inhibition
dTTP
-
0.1 mM, 30% inhibition
dUTP
-
-
EDTA
-
-
Ethoxyformic anhydride
-
deoxyguanosine slightly protects
GDP
-
1 mM, 50% inhibition at pH 6.0
gemcitabine
-
-
GMP
-
weak inhibition
guanosine
-
-
IDP
-
1 mM, 58% inhibition at pH 6.0
Iodine
-
-
N-ethylmaleimide
-
-
p-mercuribenzoate
-
-
ribavirin
-
-
Rose Bengal mediated photooxidation
-
deoxyguanosine protects
-
UMP
-
0.1 mM, 24% inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CTP
-
activation, with ATP as substrate at pH 7
dCDP
-
slight stimulation, with ATP as substrate
dTDP
-
1 mM, 620% activation of mitochondrial deoxyguanosine kinase, positive modulation with ATP as substrate
dUTP
-
activation, with ATP as substrate, at pH 7
Sodium diphosphate
-
1 mM, 120% stimulation of mitochondrial deoxyguanosine kinase
Triton X-100
UDP
-
1 mM, 310% activation of mitochondrial deoxyguanosine kinase, with ATP as substrate
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019
1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
-
pH 7.6, 37C, recombinant enzyme
0.0026 - 0.5
2'-deoxyadenosine
0.0006 - 0.123
2'-deoxyguanosine
0.056
2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
-
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
0.078
2-chloro-2'-deoxyadenosine
-
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
0.008 - 0.062
2-chlorodeoxyadenosine
0.46
2-fluoro-arabinosyl-adenine
-
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
0.033
arabinosyl guanine
-
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
0.0085 - 0.78
ATP
0.035
CTP
-
pH 8.5, 37C; pH 8.5, 37C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
0.206 - 0.63
deoxyadenosine
0.34
deoxycytidine
-
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
0.00032 - 0.76
deoxyguanosine
0.012 - 0.021
deoxyinosine
7
dioxolane guanosine
-
recombinant enzyme, pH 7.6, 37C
0.08
dTTP
-
pH 7.4, 37C, mitochondrial deoxyguanosine kinase
0.046
GTP
-
pH 8.5, 37C; pH 8.5, 37C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
0.023 - 3.3
MgATP2-
2.2
MgdTTP2-
-
pH 6.0, 37C
0.092
Triphosphate
-
pH 7.5, 37C
0.006 - 0.125
UTP
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007
1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
Homo sapiens
-
pH 7.6, 37C, recombinant enzyme
0.03 - 0.07
2'-deoxyguanosine
1.41 - 2.25
2'deoxyguanosine
1.53
ATP
Bacillus subtilis
-
pH 8.5, 37C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
2.25
CTP
Bacillus subtilis
-
pH 8.5, 37C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
0.0047
deoxyadenosine
Dictyostelium discoideum
Q54UT2
-
1.41 - 3
deoxyguanosine
0.025
dioxolane guanosine
Homo sapiens
-
recombinant enzyme, pH 7.6, 37C
1.69
GTP
Bacillus subtilis
-
pH 8.5, 37C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
1.41
UTP
Bacillus subtilis
-
pH 8.5, 37C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.117
1-alpha-D-arabinofuranosylguanine
-
pH 7.4, 37C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase
0.43
8-aza-2'-deoxyguanosine
-
pH 7.4, 37C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase
1
ADP
-
pH 7.4, 37C, noncompetitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase
0.028
dAMP
-
pH 7.6, 37C, recombinant deoxyguanosine kinase
0.041
dATP
-
pH 7.6, 37C, recombinant deoxyguanosine kinase
0.34
deoxyadenosine
-
pH 8.0, 37C
0.075
deoxyinosine
-
pH 7.4, 37C, competitive vs. deoxyguanosine, mitochondrial deoxyguanosine kinase
0.00052 - 0.0087
dGDP
0.004 - 0.25
dGMP
0.00003 - 0.021
dGTP
0.031 - 0.098
dIMP
0.0001 - 0.0026
dITP
0.21 - 0.73
dTDP
0.003 - 0.063
UDP
additional information
additional information
-
product inhibition of the enzyme, overview
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000032
-
CEM lymphocytes
0.0000338
-
-
0.0000635
-
differentiated adipocytes
0.0007
-
-
0.007
-
purified recombinant enzyme, substrates deoxyguanosine and ATP
0.0103
-
-
0.023
-
substrate 2'-deoxyguanosine and ATP
0.028
-
using 9-beta-D-arabinofuranosylguanine as substrate, in the presence of 1 mM deoxycytidine, in 50 mM Tris-HCl (pH 7.6) containing 5 mM ATP, 4 mM dithiothreitol, 10 mM sodium fluoride and 5 mM MgCl2
0.04 - 0.05
-
recombinant mitochondrial deoxyguanosine kinase
0.058
-
using deoxyguanosine as substrate, in the presence of 1 mM deoxycytidine, in 50 mM Tris-HCl (pH 7.6) containing 5 mM ATP, 4 mM dithiothreitol, 10 mM sodium fluoride and 5 mM MgCl2
0.073
-
using 2-chlorodeoxyadenosine as substrate, in the presence of 1 mM deoxycytidine, in 50 mM Tris-HCl (pH 7.6) containing 5 mM ATP, 4 mM dithiothreitol, 10 mM sodium fluoride and 5 mM MgCl2
0.54
-
enzyme in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyguanosine
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
8.1
-
purified recombinant enzyme, substrate 2'-deoxyguanosine; recombinant deoxyguanosine kinase
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
substrate deoxyguanosine
5.5
-
-
6
-
cosubstrate ATP
6.8
-
cosubstrate ATP, addition of dTTP
7
-
cosubstrate dTTP
7.4
-
cosubstrates dTTP or UTP
7.5
-
assay at
8.5
-
-
9
-
; broad optimum
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9
-
approx. 20% of maximal activity at pH 4.5 and pH 6.5 respectively, approx. 10% of maximal activity at pH 9.0
5
-
rapid decrease of activity below
5 - 9
-
approx. 55% of maximal activity at pH 5.5 and pH 9, plateau with approx. 85% of maximal activity at pH 6.0-7.5
5.6 - 9
-
more than 80% of maximal activity at pH 7.5 and pH 11.5, respectively, 60% of maximal activity at pH 6.0, complete loss of activity below pH 5.6
6 - 11.5
-
over 80% of maximal activity at pH 7.5 and pH 11.5, about 60% of maximal activity at pH 6.0, enzyme is completely inactive below pH 5.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
37 - 40
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 53
-
17% loss of activity at 53C, 50% loss of activity at 30C and 53C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
3fold increase when cells start to proliferate
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
; 3fold increase when cells start to proliferate
Manually annotated by BRENDA team
-
; no increase in activity when cells proliferate
Manually annotated by BRENDA team
-
; 3fold increase when cells start to proliferate
Manually annotated by BRENDA team
-
cell lines PanC-1 and MIA PaCa-2
Manually annotated by BRENDA team
additional information
-
enzyme is expressed in most tissues, enzyme expression throughout the cell cycle
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
deoxyguanosine kinase 2
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
additional information
the enzyme leaks out into the cytosol during cell apoptosis
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
glycerol density gradient centrifugation
49000
-
recombinant deoxyguanosine kinase, gel filtration; recombinant enzyme, gel filtration
56000
-
mitochondrial deoxyguanosine kinase, equilibrium sedimentation centrifugation
58000
-
gel filtration
58500
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ternary and quarternary structure analysis, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme in complex with ATP
purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 7 mg/ml protein, 5 mM MgCl2, and 5 mM ATP, mixed with an equal volume of cyrstallization solution containing 0.1 M Na-citrate, pH 6.5, 0.2 M ammonium acetate, and 30% w/v PEG 4000, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement method
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
95% loss of activity after 30 min at 37C
640551
7
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most stable
640551
11
-
50% loss of activity after 30 min at 37C
640551
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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at least 4 h stable in the presence of Triton X-100, t1/2: 30 min at pH 11, inactivation within 30 min at pH 5.5
40
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denaturation above 40C, mitochondrial deoxyguanosine kinase
additional information
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Triton X-100, 0.02%, stabilizes markedly against thermal inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ATP or MgATP2- stabilize, not Mg2+ alone
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ATP stabilizes the enzyme
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bovine serum albumin stabilizes the enzyme in solution
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complete inactivation after freezing at -20C or -70C
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highly unstable at 0C in diluted form, no loss of activity in the presence of bovine serum albumin
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loss of activity in dilute solution
Triton X-100 and ATP stabilize
Triton X-100, 0.02%, stabilizes markedly against thermal inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, ammonium sulfate precipitate in 100 mM M Tris-acetate buffer, pH 8, 0.025 M 2-mercaptoethanol, at least 4 months
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-20C, more than 1 mg/ml protein, 50% glycerol, 1 year, no loss of activity
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-20C, purified recombinant enzyme at 1 mg/ml, Tris-HCl, pH 7.8, 50% v/v glycerol, at least 1 year without loss of activity
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-80C, stable for at least 1 year
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0C, the diluted enzyme is highly unstable, but in presence of 1 mg/ml bovine serum albumin, the diluted enzyme is stable for months
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4C, 1 week, 50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, affinity chromatography on AMP-Sepharose and Blue-Sepharose, DEAE-cellulose, Sephadex G-75
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enzyme activity is stabilized during purification in presence of Triton X-100 and ATP
mitochondrial deoxyguanosine kinase, one-step purification via deoxyguanosine-3'-(4-aminophenylphosphate)-Sepharose affinity chromatography
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mitochondrial deoxyguanosine kinase, Sephacryl S-200, Blue Sepharose CL6B
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pH 5.5, ammonium sulfate, DEAE-Sephadex
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pH 5.7, streptomycin sulfate, ammonium sulfate
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recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose; recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity
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recombinant enzyme, metal affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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expression in Escherichia coli; gene yaaG of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
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expression of dAK/dGK in Escherichia coli
expression of His-tagged deoxyguanosine kinase in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of wild-type and mutant L250S enzymes in Escherichia coli with similar expression levels, the mutant enzyme is poorly soluble
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expresssion of mitochondrial deoxyguanosine kinase-green fluorescent protein fusion in pancreatic cancer cells
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gene is localized on chromosome 2q13
genetic organization, located on chromosome 2p13, expression as GFP-fusion protein in CHO cells
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quantitative real-time-PCR
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A2S
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resulting from a base change G to T at position 4, a G base change immediately after the start codon is likely to affect start codon recognition by the ribosome the patient has an additional base change G to A at position 591 results in skipping of exon 4, very low residual activity
D255Y/D255Y
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the mutation is associated with deoxyguanosine kinase deficiency
E165V
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naturally occuring enzyme mutation involved in mitochondrial depletion syndrome
E227K
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site-directed mutagenesis, mutation is naturally found in patients, unaltered Km but very low Vmax for all substrates compared to the wild-type enzyme
F256X
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naturally occuring enzyme mutation involved in mitochondrial depletion syndrome
H226R
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the mutation is associated with a clinical phenotype that consists of neonatal liver failure, nystagmus and hypotonia
K51Q
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the p.K51Q alteration changes the basic amino acid lysine to the uncharged polar amino acid glutamine in the ATP binding site and is expected to interfere with enzymatic function, the mutation is associated with a clinical phenotype that consists of neonatal liver failure, nystagmus and hypotonia
L250S
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naturally occurring mutation in a patient with mitochondrial DNA depletion syndrome, patient shows rapidly progressing, early onset fatal liver failure associated with profoundly decreased mtDNA levels in liver and, to a lesser extent, in skeletal muscle, mutation is introduced into the enzyme cDNA via site-directed mutagenesis, the mutant enzyme shows 0.5% remaining activity compared to the wild-type enzyme
L250S/M1T
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the mutation is associated with deoxyguanosine kinase deficiency
L266R
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10% residual activity; father of patient with double mutant, 42 of the mean control value for 2'-deoxyguanosine, and 41 of the mean control value for 2'-deoxyadenosine
M1I
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naturally occuring enzyme mutation involved in mitochondrial depletion syndrome
M1V/M1I
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the mutation is associated with deoxyguanosine kinase deficiency
N46S/L266R
R118C
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the mutation is associated with a clinical phenotype that consists of neonatal liver failure, nystagmus and hypotonia
R142K
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site-directed mutagenesis, mutation is naturally found in patients, low activity with dG, no activity with dA compared to the wild-type enzyme
R142K/E227K
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the mutation is associated with deoxyguanosine kinase deficiency
S52F/S52F
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the mutation is associated with deoxyguanosine kinase deficiency
W178X
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the mutation is associated with a clinical phenotype that consists of neonatal liver failure, nystagmus and hypotonia
W65X
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the mutation is associated with a clinical phenotype that consists of neonatal liver failure, nystagmus and hypotonia
Y191C
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the mutation is associated with a clinical phenotype that consists of neonatal liver failure, nystagmus and hypotonia
D78A
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no deoxyguanosine kinase activity
D78E
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no deoxyguanosine kinase activity
D78N
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no deoxyguanosine kinase activity
D84A
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1% of wild-type activity
D84E
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41% of wild-type activity, not inhibited by dGTP and dATP
D84N
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5% of wild-type activity
R79k
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50% of wild-type deoxyguanosine kinase activity
D84A
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1% of wild-type activity
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D84E
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41% of wild-type activity, not inhibited by dGTP and dATP
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D84N
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5% of wild-type activity
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additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Show AA Sequence (413 entries)
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