Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E227K | site-directed mutagenesis, mutation is naturally found in patients, unaltered Km but very low Vmax for all substrates compared to the wild-type enzyme | Homo sapiens |
additional information | C-terminally truncated mutants are catalytically inactive, naturally occuring genetic defects/mutations, overview | Homo sapiens |
R142K | site-directed mutagenesis, mutation is naturally found in patients, low activity with dG, no activity with dA compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
2'-deoxyguanosine | pH 7.6, recombinant wild-type enzyme | Homo sapiens | |
0.0052 | - |
2'-deoxyguanosine | pH 7.6, recombinant mutant E227K | Homo sapiens | |
0.0085 | - |
ATP | pH 7.6, recombinant wild-type enzyme, with 2'-deoxyguanosine | Homo sapiens | |
0.014 | - |
ATP | pH 7.6, recombinant mutant E227K, with 2'-deoxyguanosine | Homo sapiens | |
0.0974 | - |
ATP | pH 7.6, recombinant mutant R142K, with 2'-deoxyguanosine | Homo sapiens | |
0.123 | - |
2'-deoxyguanosine | pH 7.6, recombinant mutant R142K | Homo sapiens | |
0.242 | - |
ATP | pH 7.6, recombinant mutant E227K, with 2'-deoxyadenosine | Homo sapiens | |
0.269 | - |
ATP | pH 7.6, recombinant wild-type enzyme, with 2'-deoxyadenosine | Homo sapiens | |
0.414 | - |
2'-deoxyadenosine | pH 7.6, recombinant mutant E227K | Homo sapiens | |
0.467 | - |
2'-deoxyadenosine | pH 7.6, recombinant wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | Homo sapiens | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | Homo sapiens | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
additional information | Homo sapiens | th enzyme catalyzes the initial phosphorylation of purine deoxynucleosides, enzyme deficiency leads to severe mitochondrial DNA depletion diseases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + deoxyguanosine = ADP + dGMP | active site residues are Glu70, Lys142, and Arg142 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic efficiencies of wild-type and muztant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
additional information | th enzyme catalyzes the initial phosphorylation of purine deoxynucleosides, enzyme deficiency leads to severe mitochondrial DNA depletion diseases | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DGK | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |