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Literature summary for 2.7.1.113 extracted from

  • Hong, Y.S.; Ma, G.T.; Ives, D.H.
    Directed mutagenesis of deoxyguanosine site at arginine 79 up-regulates turnover on deoxyadenosine kinase subunit of heterodimeric enzyme from Lactobacillus acidophilus R26 (1995), J. Biol. Chem., 270, 6602-6606.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D78A no deoxyguanosine kinase activity Lactobacillus acidophilus
D78E no deoxyguanosine kinase activity Lactobacillus acidophilus
D78N no deoxyguanosine kinase activity Lactobacillus acidophilus
R79k 50% of wild-type deoxyguanosine kinase activity Lactobacillus acidophilus

Inhibitors

Inhibitors Comment Organism Structure
dGTP
-
 Lactobacillus acidophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 deoxyguanosine pH 8.0, 20°C Lactobacillus acidophilus
0.76
-
 deoxyguanosine pH 8.0, 20°C, R79K mutant enzyme Lactobacillus acidophilus

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyguanosine
-
 Lactobacillus acidophilus ADP + dGMP
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0019
-
 dGTP pH 8.0, 20°C, vs. ATP Lactobacillus acidophilus
0.00333
-
 dGTP pH 8.0, 20°C, vs. deoxyguanosine Lactobacillus acidophilus