Information on EC 2.7.1.107 - diacylglycerol kinase (ATP)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.107
-
RECOMMENDED NAME
GeneOntology No.
diacylglycerol kinase (ATP)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Glycerolipid metabolism
-
-
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
phosphatidate metabolism, as a signaling molecule
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1,2-diacyl-sn-glycerol 3-phosphotransferase
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
CAS REGISTRY NUMBER
COMMENTARY hide
60382-71-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain HL5
-
-
Manually annotated by BRENDA team
strain HL5
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
-
-
-
Manually annotated by BRENDA team
Sus scrofa DGKalpha
DGKalpha
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dipalmitoyl-sn-glycerol + GTP
GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
2'-deoxy-ATP + sn-1,2-dihexanoylglycerol
2'-deoxy-ADP + sn-1,2-dihexanoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ADP + sn-1,2-dihexanoylglycerol
AMP + sn-1,2-dihexanoylglycerol 3-phosphate
show the reaction diagram
-
MgADP- is a very poor phosphoryl donor
-
-
?
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-diarachidonoyl-glycerol
ADP + 1,2-diarachidonoyl-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1,2-diarachidonoyl-sn-glycerol
ADP + 1,2-diarachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
ATP + 1,2-dicapryl-sn-glycerol
ADP + 1,2-dicapryl-sn-glycerol 3-phosphate
show the reaction diagram
about 140% of the activity with sn-1,2-dioleoylglycerol
-
-
?
ATP + 1,2-didecanoylglycerol
ADP + 1,2-didecanoylglycerol 3-phosphate
show the reaction diagram
-
enzyme type I: activity is 157% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 141% of the activity with rac-1,2-dioleoylglycerol
-
-
?
ATP + 1,2-didodecanoylglycerol
ADP + 1,2-didodecanoylglycerol 3-phosphate
show the reaction diagram
-
enzyme type I: activity is 107% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 227% of the activity with rac-1,2-dioleoylglycerol
-
-
-
ATP + 1,2-dihexadecanoylglycerol
ADP + 1,2-dihexadecanoylglycerol 3-phosphate
show the reaction diagram
-
enzyme type I: activity is 198% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 231% of the activity with rac-1,2-dioleoylglycerol
-
-
?
ATP + 1,2-dihexanoyl-sn-glycerol
ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-dihexanoylglycerol
ADP + 1,2-dihexanoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1,2-dilinoleoyl-sn-glycerol
ADP + 1,2-dilinoleoyl-sn-glycerol
show the reaction diagram
-
-
-
?
ATP + 1,2-dioctanoyl-sn-glycerol
ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-dioctanoylglycerol
ADP + 1,2-dioctanoylglycerol 3-phosphate
show the reaction diagram
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-dipalmitoyl-sn-glycerol
ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-ditetradecanoylglycerol
ADP + 1,2-ditetradecanoylglycerol 3-phosphate
show the reaction diagram
-
enzyme type I: activity is 200% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 262% of the activity with rac-1,2-dioleoylglycerol
-
-
-
ATP + 1,3-dioleoyl-sn-glycerol
ADP + 1,3-dioleoyl-sn-glycerol 2-phosphate
show the reaction diagram
-
low activity, about 4% of the activity with 1,2-dioleoyl-sn-glycerol
-
-
?
ATP + 1-arachidoyl-2-arachidonoyl-sn-glycerol
ADP + ?
show the reaction diagram
-
isoform DGKepsilon shows about 70% activity with 0.38 mol% 1-arachidoyl-2-arachidonoyl-sn-glycerol compared to 1-stearoyl-2-arachidonoyl-sn-glycerol
-
-
?
ATP + 1-O-hexadecyl-2-oleoyl-sn-glycerol
ADP + 1-O-hexadecyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
45.4% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol
-
-
?
ATP + 1-O-hexadecyl-2-sn-acetyl glycerol
ADP + 1-O-hexadecyl-2-sn-acetyl glycerol 3-phosphate
show the reaction diagram
-
about 12fold the rate of 1-O-hexadecyl-sn-glycerol phosphorylation, isoforms diacylglycerol kinase alpha, beta, gamma, delta1, delta1, zeta, jota, theta
-
-
?
ATP + 1-O-hexadecyl-sn-glycerol
ADP + 1-O-hexadecyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-O-hexanoyl-2-arachidonoyl-sn-glycerol
ADP + 1-O-hexanoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
preferred by isozyme DGKzeta and isozyme DGKalpha
-
-
?
ATP + 1-O-hexanoyl-2-oleoyl-sn-glycerol
ADP + 1-O-hexanoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-oleoyl-2-palmitoyl-sn-glycerol
ADP + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
about 85% of the activity with sn-1,2-dioleoylglycerol, DGKksi
-
-
?
ATP + 1-palmitoyl-2-arachidonoyl-sn-glycerol
ADP + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1-palmitoyl-2-linoleoyl-sn-glycerol
ADP + 1-palmitoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
enzyme type I: activity is 116% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 86% of the activity with rac-1,2-dioleoylglycerol
-
-
?
ATP + 1-palmitoyl-2-oleoyl-sn-glycerol
ADP + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol
ADP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol
show the reaction diagram
no substrate for wild-type, but substrate for mutant R457Q
-
-
?
ATP + 1-stearoyl-2-linoleoyl-sn-glycerol
ADP + 1-stearoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1-stearoyl-2-oleoyl-sn-glycerol
ADP + 1-stearoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
ATP + 2,3-dioleoyl-sn-glycerol
ADP + 2,3-diacyl-sn-glycerol 1-phosphate
show the reaction diagram
ATP + 2-arachidonoyl-sn-glycerol
ADP + 2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
isoform DGKepsilon shows substrate specificity for sn-2 arachidonoyl-diacylglycerol
-
-
?
ATP + 2-monooleoyl-rac-glycerol
ADP + 2-monooleoyl-rac-glycerol 3-phosphate
show the reaction diagram
-
10.7% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol
-
-
?
ATP + ceramide
ADP + ceramide 3-phosphate
show the reaction diagram
ATP + rac-1,2-dioleoylglycerol
ADP + rac-1,2-dioleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + sn-1,2-dihexanoylglycerol
ADP + sn-1,2-dihexanoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + sn-1,2-dioctanoylglycerol
ADP + sn-1,2-dioctanoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + sn-1,2-dioleoylglycerol
ADP + sn-1,2-dioleoylglycerol 3-phosphate
show the reaction diagram
ATP + sn-1,3-dioleoylglycerol
ADP + ?
show the reaction diagram
about 10% of the activity with sn-1,2-dioleoylglycerol
-
-
?
GTP + dioleoylglycerol
GDP + dioleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
GTP + sn-1,2-dihexanoylglycerol
GDP + sn-1,2-dihexanoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ITP + sn-1,2-dihexanoylglycerol
IDP + sn-1,2-dihexanoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-diarachidonoyl phosphatidic acid
-
about 55% relative activity in the presence of 1.5 mol%
1-arachidoyl-2-arachidonoyl phosphatidic acid
-
about 70% relative activity in the presence of 1.5 mol%
1-palmitoyl-2-arachidonoyl phosphatidic acid
-
about 50% relative activity in the presence of 1.5 mol%
1-stearoyl-2-arachidonoyl phosphatidic acid
-
about 48% relative activity in the presence of 1.5 mol%
1-stearoyl-2-oleoyl phosphatidic acid
-
about 70% relative activity in the presence of 1.5 mol%
2,3-dioleoylglycerol
-
uncompetitive inhibition of isoforms diacylglycerol kinase alpha and zeta, no inhibition of isoform epsilon. Binds to a site on the alpha and zeta isoforms that is exposed as a consequence of the substrate binding to the active site, the chiral specificity of the isoforms thus mimicks the substrate specificity
2-arachidonoyl glycerol
inhibitor for both of the epsilon or the zeta isoforms of diacylglycerol kinase; inhibitor for both of the epsilon or the zeta isoforms of diacylglycerol kinase
2-oleoyl glycerol
inhibits diacylglycerol kinase epsilon less than does 2-arachidonoyl glycerol; shows similar inhibitory potency for diacylglycerol kinase zeta as 2-arachidonoyl glycerol
adenosine
-
0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV
adenosine 5'-tetraphosphoryl-3-O-(1,2-dihexanoyl)-sn-glycerol
-
-
alpha-thrombin
-
apparent binding parameters of diacylglycerol kinase theta increase following alpha-thrombin stimulation
-
AMP
-
0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV
antineutrophil cytoplasmic antibody
-
selective activation of diacylglycerol kinase
-
ATPgammaS
cAMP
-
0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV
ceramide
-
-
deoxycholate
diacylglycerol
-
above 3.4 mol%, substrate inhibition
dioctanoylglycerol
-
above 3.4 mol%
dioleoylglycerol
-
above 3.4 mol%
ethanol
-
0.5%, 20% inhibition
GTPgammaS
-
0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV
LiCl
more than 50% inhibition at above 200 mM
NaCl
more than 50% inhibition at above 200 mM
Nonidet
-
0.5%, 45% inhibition
octyl glucoside
-
enzyme form DGK-I, DGK-II and DGK-III
phorbol ester
-
activation of protein kinase C which inhibits diacylglycerol kinase epsilon binding to retinoblastoma protein. Mimicking of protein kinase C phosphorylation of serine residues by S/D mutations but not S/N mutations within the MARCKS phosphorylation site domain also prevents binding to retinoblastoma protein
phorbol myristate acetate
-
induces the interaction of diacylglycerol kinase gamma with beta2-chimaerin. Simultaneous addition of H2O2 and phorbol myristate acetate synergistically enhances the interaction with concomitant translocation of beta2-chimaerin from cytoplasm to the plasma membrane
phosphate
-
-
phosphatidate
-
moderate
phosphatidic acid
-
competitive inhibition
phosphatidylcholine
-
enzyme form DGK-I, DGK-II and DGK-III
phosphatidylglycerol
-
inhibits phosphatidylcholine-dependent kinase activity
phosphatidylinositol
-
2.5 mol% results in 50% inhibition of the phosphatidylcholine-dependent kinase activity
phosphatidylinositol 4,5-bisphosphate
potent inhibitor
phosphatidylinositol-(4,5)-bisphosphate
potent inhibitor; potent inhibitor
quercetin
-
0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV
R59022
R59949
RhoA
-
V14-RhoA, strong binding to the C-terminal catalytic domain, negative regulation
Sodium deoxycholate
at 5 mM
sphingosine
-
inhibits the 150000 Da enzyme
thrombin
-
stimulation results in an increase in the apparent KM of DGKtheta at low concentrations of substrate dioleoylglycerol. Increasing the bulk concentration of dioleoylglycerol returns the apparent KM to the basal value
-
Triton N-101
Triton X-100
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(S)-2-amino-2-((S)-6-octyl-1,2,3,4-tetrahydronaphthalen-2-yl)propan-1-ol
-
analogue of FTY720, 0.01 mM increase phosphorylation of 1-O-hexadecyl-sn-2-acetyl glycerol by isoform diacylglycerol kinase alpha about 3.5fold.
1,3-dioleoylglycerol
-
activates
1-monooleoylglycerol
-
activates
1-O-alkylphosphatidylcholine
-
half-maximal activation at 21.9 mol%
1-palmitoyl-2-oleoylglycerophosphocholine
-
activates
4beta-phorbol-12-myristate-13-acetate
enhances voltage-dependent opening of wild-type and cAMP/H+-uncoupled hyperpolarization activated, cyclic nucleotide-regulated channels. 4beta-Phorbol-12-myristate-13-acetate exerts its effects on channel gating via sequential activation ofprotein kinase C and diacylglycerol kinase coupled with upregulation of mitogen-activated protein kinase and phospholipase A2
acidic phospholipids
-
activation in vitro
-
benzothiadiazole
-
activation of the expression of diacylglycerol kinase OsDAGK1
bis-phosphatidic acid
-
half-maximal activation at 3.9 mol%
-
cAMP
-
stimulates nuclear diacylglycerol kinase catalytic activity
cardiolipin
cholesterol
cholesterol 3-sulfate
-
activates
D-glucose
-
exposure of L6 cell myotubes overexpressing human insulin receptors to 25 mM glucose for 5 min decreases the intracellular levels of diacylglycerol, paralleled by transient activation of diacylglycerol kinase and of insulin receptor signaling. Following 30-min exposure, both diacylglycerol levels and diacylglycerol kinase activity return close to basal levels. Glucose exposure redistributes diacylglycerol kinase isoforms alpha and delta, from the prevalent cytosolic localization to the plasma membrane fraction
deoxycholate
Detergent
-
no activity in absence of detergent
-
di-O-hexadecylphosphatidylcholine
-
half-maximal activation at 13.5 mol%
diacylglycerol 3-phosphate
-
the enzyme apoprotein is attributed to a novel feedback activation involving diacylglycerol 3-phosphate
dilauroyl-N,N-dimethylglycerophosphoethanolamine
-
activates
dilauroyl-N-methylglycerophosphoethanolamine
-
activates
dilauroylglycerophosphocholine
-
activates
dilauroylglycerophosphoethanolamine
-
activates
dilauroylphosphatidylcholine
-
half-maximal activation at 11.9 mol%
dimethylmyristamide
-
activates
dioleoyl ethylene glycol
-
activates
dioleoyl-phosphatidylglycerol
-
-
dioleoylphosphatidylcholine
-
half-maximal activation at 10.4 mol%
dioleoylphosphatidylglycerol
-
half-maximal activation at 6.3 mol%
dipalmitoylphosphatidic acid
-
activates only in presence of Triton X-100
endothelin-1
-
activates diacylglycerol kinase in caveolae/rafts and noncaveolae/rafts of mesenteric arteries. Activation does not depend on phosphatidylinositol 3-kinase. In response to norepinephrin, but not to epithelin-1, protein kinase PKB translocates to caveolae/rafts
FTY720
-
in presence of 0.01 mM FTY720, phosphorylation of 1-O-hexadecyl-sn-2-acetyl glycerol by isoforms diacylglycerol kinase alpha, beta or gamma is 3fold increased
H2O2
-
endogenous nuclear diacylglycerol kinase zeta rapidly translocates to the cytoplasm following H2O2 treatment
hepatocyte growth factor
-
induces diaclyglycerol kinase activity, which is required for cell invasiveness
-
hexadecanol
-
activates
hexadecyl phosphorylcholine
-
half-maximal activation at 17.3 mol%
hexadecylphosphorylcholine
-
activates
lauryl maltoside
-
activates in presence of 11 mM Triton X-100
Lipid
-
purified enzyme is completely inactive unless a lipid is added to the assay buffer containing Triton X-100
lysophosphatidylcholine
-
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
lysophosphatidylethanolamine
-
activates
methyl myristate
-
activates
myristoylcholine chloride
-
activates
myristyl acetate
-
activates
n-hexyl beta-D-glucoside
-
activates in presence of 11 mM Triton X-100
nitrododecane
-
activates
norepinephrine
-
stimulates an increase in diacylglycerol kinase activity in caveolae/rafts of mesenteric arteries. Activation depends on phosphatidylinositol 3-kinase. In response to norepinephrin, but not to epithelin-1, protein kinase PKB translocates to caveolae/rafts
octyl acetate
-
activates
octyl beta-glucoside
-
activates in presence of 11 mM Triton X-100
oleic acid
-
activates only in presence of Triton X-100
oleoylcholine chloride
-
activates
-
palmitic acid
-
activates only in presence of Triton X-100
phorbol-12-myristate-13-acetate
diacylglycerol kinase zeta activity at the T cell receptor is enhanced by phorbol-12-myristate-13-acetate cotreatment
phosphatidic acid
phosphatidyl glycerol
-
good activator
phosphatidylcholine
phosphatidylcholine plasmalogen
-
half-maximal activation at 7.3 mol%
-
phosphatidylethanolamine
phosphatidylglycerol
-
effective stimulation
phosphatidylinositol
phosphatidylinositol 4,5-bisphosphate
-
highly stimulating
phosphatidylserine
Phospholipid
platelet-activating factor
-
half-maximal activation at 22.4 mol%
rac-1,2-dioleoylglycero-3-sulfate
-
half-maximal activation at 2.7 mol%
sn-1,2-dioleoylglycerol
-
activates
sn-1,3-dioleoylglycerol
-
activates
Sodium cholate
-
enhances activity of DGK I and DGK IV
Sodium deoxycholate
-
enhances activity of DGK I and DGK IV
Sodium dodecyl sulfate
-
activates
sodium hexadecyl sulfate
-
half-maximal activation at 9.8 mol%
Span-20
-
activates
sphingomyelin
-
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
sphingosine
stearic acid
-
activates only in presence of Triton X-100
stearoyllysophosphatidylcholine
-
half-maximal activation at 15.8 mol%
Triton X-100
-
activates
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 0.14
1,2-diarachidonoyl-glycerol
0.45
1,2-diolein
-
pH 7.5, 30C
0.125
1,2-dioleoyl-sn-glycerol
pH 7.5, 25C
0.07 - 0.09
1-stearoyl-2-arachidonoyl-glycerol
4.2
2'-deoxy-ATP
-
pH 6.8, 30C, reaction with sn-2,3-dihexanoylglycerol
1
ADP
-
about, pH 6.8, 30C, reaction with sn-2,3-dihexanoylglycerol
0.1 - 4.8
ATP
0.23
ceramide
-
pH 6.6, 25C
0.25
diacylglycerol
-
pH 7.5, 25C
0.05 - 0.08
diolein
0.9
dioleoylglycerol
-
pH 7.5, 25C
0.03 - 8.7
GTP
5.9
ITP
-
pH 6.8, 30C, reaction with sn-2,3-dihexanoylglycerol
0.12 - 2.1
MgATP2-
0.1 - 0.125
sn-1,2-dioleoylglycerol
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032 - 2.43
1,2-dioleoyl-sn-glycerol
0.162 - 5.7
1-stearoyl-2-arachidonoyl-sn-glycerol
0.087 - 2.8
1-stearoyl-2-linoleoyl-sn-glycerol
0.53 - 2.35
1-stearoyl-2-oleoyl-sn-glycerol
0.01 - 23
ATP
additional information
additional information
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.095
ADP
-
pH 7.5, 25C
0.102
ATP
-
pH 7.5, 25C
0.06
CDP
-
pH 7.5, 25C
0.48
CTP
-
pH 7.5, 25C
0.058
GDP
-
pH 7.5, 25C
0.123
GDPbetaS
-
pH 7.5, 25C
0.081
GTP
-
pH 7.5, 25C
0.038
IDP
-
pH 7.5, 25C
0.51
ITP
-
pH 7.5, 25C
2.5
phosphate
-
pH 7.5, 25C
0.043
UDP
-
pH 7.5, 25C
0.26
UTP
-
pH 7.5, 25C
additional information
additional information
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00001
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1-oleoyl-rac-glycerol
0.0002 - 0.018
-
substrate specificity and catalytic activity level of the isozymes at different liposome compositions
0.0004
-
mutant enzyme D271A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles; mutant enzyme D68A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles; mutant enzyme E273A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0008
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1,3-dioleoyl-sn-glycerol
0.0019
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1,2-dioleoyl-sn-glycerol
0.0021
-
recombinant MBP-fusion enzyme in recombinant insect cell extract, substrate 1-stearoyl-2-arachidonoyl-sn-glycerol
0.0023
-
mutant enzyme N96A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0024
-
mutant enzyme D124A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0028
-
mutant enzyme K15A/K165A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0031
-
mutant enzyme T94A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0036
-
mutant enzyme D97A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0062
-
mutant enzyme D216A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0122
-
mutant enzyme K15A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0139
-
mutant enzyme K165A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0168
-
mutant enzyme R100A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0249
-
wild type enzyme, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
0.0256
-
mutant enzyme R20A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles
2.207
-
enzyme form DGK-II
4.74
-
enzyme form DGK-I
4.8
substrate 1,2-dioctanoyl-sn-glycerol, pH 7.0, 25C
5.187
-
enzyme form DGK IV
6.753
-
enzyme form DGK-III
6.913
-
-
7.5
-
purified solubilized MBP-fusion wild-type enzyme
7.7
-
1500000 Da enzyme
15
-
80000 Da enzyme
23
-
purified recombinant mutant W25L
26
-
purified recombinant mutant W18L/W25L/W47L
36
-
purified recombinant mutant W18L/W25L/W47L/W117L
42
-
purified recombinant mutant W18L/W25L
61
-
purified recombinant mutant W18L
77
-
purified recombinant mutant W18L/W47L/W117L
89
substrate 1,2-dioleoyl-sn-glycerol, pH 7.0, 25C
102
-
purified recombinant wild-type enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
-
enzyme from supernantant fraction
6.3 - 8.3
-
-
6.4
-
enzyme from microtubule
6.9
-
assay at
7.5 - 8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7.5
-
pH 4.5: about 50% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from supernatant. pH 4.5: about 55% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from microtubuli
6 - 9
-
about 50% of maximal activity at pH 6.0 and 9.0
6 - 8.5
-
pH 6.0: about 40% of maximal activity, pH 8.5: about 50% of maximal activity
6.5 - 7.8
rapid decrease below pH 6.5 and above pH 7.8
6.5 - 7
-
rapid decrease of activity below pH 6.5, pH 7: optimum
6.5 - 8.5
-
pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity
6.5 - 9
-
pH 6.5: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, phosphatidylcholine-dependent activity
6.8 - 9
-
pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoelectric focusing
additional information
-
it remains unclear whether the multiple species, observed by isoelectric focusing represent distinct isoforms of the enzyme, or are modified versions of the type I and type II enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
isozymes DGKalpha and DGKzeta
Manually annotated by BRENDA team
-
neuron, DGK-II
Manually annotated by BRENDA team
DGKeta1 and DGKeta2
Manually annotated by BRENDA team
-
ras-transformed
Manually annotated by BRENDA team
-
enzyme expression remained almost unchanged in granulocytic differentiation pathway; enzyme expression remains almost unchanged in granulocytic differentiation pathway
Manually annotated by BRENDA team
-
DGK-IIdelta
Manually annotated by BRENDA team
-
level of DGKgamma is rapidly and markedly decreased upon cellular differentiation into macrophages; levels of DGKgamma mRNA/protein is rapidly and markedly decreased upon cellular differentiation into macrophages
Manually annotated by BRENDA team
especially cauline
Manually annotated by BRENDA team
-
mammary carcinoma
Manually annotated by BRENDA team
-
breast cancer cell
Manually annotated by BRENDA team
-
isozyme DGKgamma, day 3-14 after birth
Manually annotated by BRENDA team
-
DGKbeta is expressed in medium spiny neurons constituting the striatonigral and striatopallidal pathways, whereas striatal interneurons are below the detection threshold
Manually annotated by BRENDA team
-
; expression of isoform diacylglycerol kinase alpha in several melanoma cell lines but not in noncancerous melanocytes
Manually annotated by BRENDA team
-
diaclyglycerol kinase activity is reduced by oxidative stress in glomerular mesangial cells cultured under high glucose conditions. Antioxidants, including D-alpha-tocopherol and probucol may improve hyperglycemia-induced diacylglycerol-protein kinase C activation by enhancing diacylglycerol kinase activity
Manually annotated by BRENDA team
-
DGKzeta-immunoreactivity is clearly detected in Iba1-immunoreactive cells of an oval or ameboid shape in the scar region, which represent activated microglia and/or macrophages. DGKzeta-immunoreactivity is not detected in Iba1-immunoreactive, resting microglia of ramified and dendritic configuration in the intact cortex
Manually annotated by BRENDA team
-
ventricular myocyte
Manually annotated by BRENDA team
-
expression of diacylglycerol kinase isoform alpha, delta, epsilon, zeta, or theta mRNA
Manually annotated by BRENDA team
-
activation of the adrenocorticotropin/cAMP signal transduction cascade rapidly increases nuclear diacylglycerol kinase activity and phosphatidic acid production. LXXLL motifs in diacylglycerol kinase theta mediate a direct interaction of nuclear receptor steroidogenic factor 1 with the kinase and may facilitate binding of phosphatidic acid to the receptor
Manually annotated by BRENDA team
-
weak labeling in the neocortex
Manually annotated by BRENDA team
DGKeta1
Manually annotated by BRENDA team
-
DGKbeta is the major isozyme in the pituitary gland, the signals are also detected intensely for isoform DGKzeta, moderately for DGKepsilon and faintly for DGKalpha, the signals for isoforms DGKgamma and DGKiota are below the detection level
Manually annotated by BRENDA team
-
isoenzyme DGK-I, DGK-II and DGK-II
Manually annotated by BRENDA team
-
delay of isozyme DGKgamma
Manually annotated by BRENDA team
-
expression is induced by benzothiadiazole, and by infection with Magnaporthe grisea. In benzothiadiazole-treated rice seedlings, expression is induced earlier and at a higher level than in water-treated control seedlings after inoculation with Magnaporthe grisea
Manually annotated by BRENDA team
and brain, main localization in male adult
Manually annotated by BRENDA team
-
dark-grown
Manually annotated by BRENDA team
-
DGKiota immunoreactivity is distributed solely in the cytoplasm of most of the dorsal root ganglion; DGKzeta is detected heterogeneously in the nucleus and cytoplasm of small neurons of the dorsal root ganglion with variable levels of distribution, whereas it is detected exclusively in the cytoplasm of large neurons
Manually annotated by BRENDA team
-
DGKalpha
Manually annotated by BRENDA team
-
levels of DGKgamma mRNA/protein is rapidly and markedly decreased upon cellular differentiation into macrophages
Manually annotated by BRENDA team
-
level of DGKgamma is rapidly and markedly decreased upon cellular differentiation into macrophages
Manually annotated by BRENDA team
additional information