Information on EC 2.4.1.149 - N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.1.149
-
RECOMMENDED NAME
GeneOntology No.
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycosaminoglycan biosynthesis - keratan sulfate
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Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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terminal O-glycans residues modification
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine 3-beta-N-acetyl-D-glucosaminyltransferase
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoprotein and other glycoproteins and oligosaccharides.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-
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B3gnt1
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B3gnt1
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beta-1,3-N-acetylglucosaminyltransferase 1
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beta1,3-N-acetylglucosaminyltransferase
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beta1,3-N-acetylglucosaminyltransferase-7
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beta1-3 N-acetylglucosaminyltransferase-1
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beta3-N-acetylglucosaminyltransferase 1
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beta3GlcNAcT
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beta3Gn-T7
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beta3GnT1
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beta3GnT7
Q8K0J2
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beta3GnT7
Mus musculus beta3GnT7
Q8K0J2
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Galbeta1-->4GlcNAc-R beta1-->3 N-acetylglucosaminyltransferase
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GnT-I
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i-GlcNAc transferase
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i-GlcNAcT
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IGnT
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N-acetyllactosamine beta(1-3)N-acetylglucosaminyltransferase
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poly-N-acetyllactosamine extension enzyme
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UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
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UDP-GlcNAc:Galbeta1-->4GlcNAcbeta-Rbeta1-->3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:Galbeta1-4Glc(NAc) beta-1,3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:GalR, beta-D-3-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
85638-39-7
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
newborn calf
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
beta3GnT7
SwissProt
Manually annotated by BRENDA team
Mus musculus beta3GnT7
beta3GnT7
SwissProt
Manually annotated by BRENDA team
Sprague Dawley rats
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
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beta3GnT1 is required for laminin-binding glycan synthesis through formation of a complex with LARGE, thus regulating the function of LARGE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoproteins and other glycoproteins and oligosaccharides, GlcNAc residues are introduced to position C-3 of the terminal galactose of the glycoprotein
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
highly specific for acceptor oligosaccharides and glycoproteins carrying a terminal Galbeta(1-4)GlcNAcbeta1-R unit, catalyzes the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-R sequence, Galbeta(1-4)GlcNAcbeta(1-2)(Galbeta(1-4)GlcNAcbeta(1-6))Man pentasaccharide in the acceptor structure is a requirement for optimal activity, branch specificity, branches of this pentasaccharide structure, when contained in tri- and tetraantennary oligosaccharides, are highly preferred over other branches for attachment of the 1st and 2nd mol of GlcNAc into the acceptor molecule, enzyme also shows activity towards oligosaccharides related to blood group I- and i-active polylactosaminoglycans
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
O43505
iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins
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?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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might function in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group i antigenic structures, enzyme controls the synthesis of linear chain types by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R primer structure present on glycoprotein or glycolipid yielding a GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R sequence
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UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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involved in the biosynthesis of blood group precursors, enzyme might control the biosynthesis of the linear carbohydrate chain by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R structure present on oligosaccharides, glycosylceramides and glycoproteins
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UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
O43505
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
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UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
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UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
B3GNT1 displays an in vitro preference for the GlcNAcbeta1->2Man branch
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-
?
UDP-N-acetyl-D-glucosamine + Fucalpha(1->2)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)[Fucalpha(1->2)]Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
32% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
O43505
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
i.e. lacto-N-neotetraose, 117% of activity with N-acetyllactosamine
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-6)(Galbeta(1-4)GlcNAcbeta(1-2))Manalpha(1-6)Manbeta-octyl
?
show the reaction diagram
-
regioselectivity of enzyme, favored site of GlcNAc addition is the lower beta1,2-branch over the beta1,6-branch by a 3:1 ratio resulting in a mixture of heptasaccharides
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta-p-nitrophenol
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-p-nitrophenol
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
UP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
show the reaction diagram
-
76% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-pNP
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-pNP
show the reaction diagram
-
poor acceptor, 23% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
28% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)Glcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)Glcbeta-O(CH2)3N3
show the reaction diagram
-
65% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAc
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
show the reaction diagram
-
73% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
107% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
90% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
57% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
show the reaction diagram
-
104% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
100% activity
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
26% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
-
i.e. L2L4, 72% activity compared to L2L2
-
-
-
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
-
i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate
-
-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
-
mixture of the pentasaccharide product, 27 mol%, and the unreacted tetrasaccharide, 73 mol%
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OMe
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OR
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OR
show the reaction diagram
-
i.e. N,N-diacetyllactosediamine-OR
only 12.5% of the substrate is converted into the product, which is unusually resistant towards jackbean beta-N-acetylhexosaminidase
?
UDP-N-acetyl-D-glucosamine + glycolipid
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
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-
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UDP-N-acetyl-D-glucosamine + keratan sulfate
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
-
polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
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-
-
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
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-
-
UDP-N-acetyl-D-glucosamine + O-glycan
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
O43505
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
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-
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UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
enzyme is capable of elongation of polylactosamine i-chains
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-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
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-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
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-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
synthesis of N-acetyllactosamine repeats in Asn-linked oligosaccharides is enhanced by an increase of enzyme
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-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
poly-N-acetyllactosamine is biosynthesized by the alternating addition of N-acetyl-D-glucosamine by UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase and Gal by UDP-Gal:betaGlcNAc beta-1,4-galactosyltransferase
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
O43505
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
-
best acceptor among the glycoproteins that contain Galbeta(1-4)GlcNAc
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
-
GlcNAc residues are introduced to position C-3 of the terminal galactose of the glycoprotein, relative high activity towards asialo-alpha1-acid glycoprotein in Novikoff ascites tumor cells
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
-
much more effective than asialo-transferrin and asialo-fetuin, asialo-alpha1-acid glycoprotein from human plasma Cohn fraction V, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
-
-
?
UDP-N-acetylglucosamine + asialo-fetuin
UDP + N-acetylglucosaminylated asialo-fetuin
show the reaction diagram
-
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
-
-
?
UDP-N-acetylglucosamine + asialo-transferrin
UDP + N-acetylglucosaminylated asialo-transferrin
show the reaction diagram
-
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
-
-
?
UDP-N-acetylglucosamine + lactoneotetraosylceramide
?
show the reaction diagram
-
lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length
-
-
?
UDP-N-acetylglucosamine + lactonorhexaosylceramide
?
show the reaction diagram
-
lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length
-
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
less physiological acceptor than N-acetyllactosamine
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
less effective acceptor than N-acetyllactosamine
-
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
70% of activity with N-acetyllactosamine
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage, 51% of activity with N-acetyllactosamine
-
?
UDP-N-acetylglucosamine + lactosylceramide
?
show the reaction diagram
-
lactosylceramide from human erythrocytes
-
-
?
UDP-N-acetylglucosamine + lactotetraosylceramide
?
show the reaction diagram
-
lactotetraosylceramide from human meconium, poor substrate
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
more physiological acceptor than lactose
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
more effective acceptor than lactose
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
more effective acceptor than lactose
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage
-
-
?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
show the reaction diagram
-
very poor substrate
-
-
?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
show the reaction diagram
-
poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure
-
-
?
Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
115% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
additional information
?
-
-
no acceptors: melibiose, gentiobiose, galactose, glucose, N-acetylgalactosamine, N-acetylglucosamine
-
-
-
additional information
?
-
-
asialo-serum glycoproteins are much more effective than O-glycoproteins as acceptors, overview over oligosaccharide substrates
-
-
-
additional information
?
-
-
transfer of GlcNAc occurs mainly to type 2 chain nonfucosylated structures, elongation of type 1 chain structure Lc4 is also detectable, no transfer to any fucosylated derivative of either type 1 or 2 chains, transfer of GlcNAc to a terminal Gal residue on a lacto-series core chain
-
-
-
additional information
?
-
-
terminal Galbeta(1-4)Glc(NAc) sequences, i.e. type II chains, are preferred substrates, no substrates: terminal Galbeta(1-3)GlcNAc sequences, i.e. type I chains, Lewis X trisaccharides, i.e. Galbeta(1-4)(Fucalpha(1-3))GlcNAc, monosaccharides, e.g. galactose, asialo-bovine submaxillary mucin that contains GalNAcalpha1-Ser/Thr and Galbeta(1-3)GalNAcalpha1-Ser/Thr
-
-
-
additional information
?
-
O43505
no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
-
additional information
?
-
-
biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia
-
-
-
additional information
?
-
-, Q8K0J2
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
-
additional information
?
-
-
the enzyme together with N-acetyllactosamine synthase, EC 2.4.1.90, catalyzes formation of linear glycans containing alternating beta-3-O-substituted residues of D-galactose and beta-4-O-substituted residues of N-acetyl-D-glucosamine, structures are present among others in glycosphingolipids or H-II type, polyglycosylceramides and polyglycosylpeptides, e.g. erythroglycan
-
-
-
additional information
?
-
-
enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line, substrate specificity, enzyme acts efficiently on keratan sulfate-related glycans, while lacto-N-tetraose and lacto-N-neo-tetraose are poor substrates, overview, product analysis
-
-
-
additional information
?
-
-
B3gnt1 null females are fertile, the B3gnt1 null males are not able to sire litters at the expected rate when mated to either wild type or B3gnt1-null females
-
-
-
additional information
?
-
-
the enzyme shows no activity on a terminal Gal with Neu5Acalpha(2->6) substitution. Terminal galactose with alpha-linkage, Galalpha(1->3)Gal or Galalpha(1->4)Gal is not an acceptor substrate for the enzyme
-
-
-
additional information
?
-
Mus musculus beta3GnT7
Q8K0J2
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
might function in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group i antigenic structures, enzyme controls the synthesis of linear chain types by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R primer structure present on glycoprotein or glycolipid yielding a GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R sequence
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
involved in the biosynthesis of blood group precursors, enzyme might control the biosynthesis of the linear carbohydrate chain by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R structure present on oligosaccharides, glycosylceramides and glycoproteins
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
O43505
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
-
-
-
UDP-N-acetyl-D-glucosamine + glycolipid
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
-
polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + O-glycan
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
O43505
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
enzyme is capable of elongation of polylactosamine i-chains
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
synthesis of N-acetyllactosamine repeats in Asn-linked oligosaccharides is enhanced by an increase of enzyme
-
-
-
additional information
?
-
-
biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia
-
-
-
additional information
?
-
-, Q8K0J2
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
-
additional information
?
-
-
the enzyme together with N-acetyllactosamine synthase, EC 2.4.1.90, catalyzes formation of linear glycans containing alternating beta-3-O-substituted residues of D-galactose and beta-4-O-substituted residues of N-acetyl-D-glucosamine, structures are present among others in glycosphingolipids or H-II type, polyglycosylceramides and polyglycosylpeptides, e.g. erythroglycan
-
-
-
additional information
?
-
-
enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line
-
-
-
additional information
?
-
-
B3gnt1 null females are fertile, the B3gnt1 null males are not able to sire litters at the expected rate when mated to either wild type or B3gnt1-null females
-
-
-
additional information
?
-
Mus musculus beta3GnT7
Q8K0J2
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
about one third of the activity compared to Mn2+, recombinant enzyme
Cd2+
-
CdCl2 slightly stimulates
Co2+
-
slightly stimulates, 12.8% as effective as Mn2+ at 10 mM
Mg2+
-
MgCl2 slightly stimulates
Mg2+
-
about one third of the activity compared to Mn2+, recombinant enzyme
Mn2+
-
MnCl2: 10-25 mM for optimal activity required
Mn2+
-
requirement
Mn2+
-
essential for activity, neither Mg2+ nor Ca2+ can substitute for Mn2+
Mn2+
-
strict requirement
Mn2+
-
MnCl2 stimulates 2.5fold, optimal concentration: 20 mM
Mn2+
-
optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+; strict requirement
Mn2+
-
required for activity, recombinant enzyme
additional information
-
not activated by Mg2+ or Ca2+
additional information
-
absolute requirement for divalent cations, not stimulated by Mg2+
additional information
-
enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
-
4-Thiouridine diphosphate
-
inhibits in a concentration-dependent manner
EDTA
-
10 mM, complete inhibition
EDTA
-
slightly, 12% inhibition at 10 mM
N-acetyllactosamine
-
strong product inhibition
UDP
-
1 mM, 70% inhibition
additional information
-
nerve growth factor stimulated PC-12 cells with reduced GnT-i activity compared to unstimulated cells, no effect in PC-12D cells
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ATP
-
omitting ATP from reaction mixture decreases activity by 87%
CHAPSO
-
activates solubilized enzyme
deoxycholate
-
activates solubilized enzyme
Triton CF-54
-
activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3%
Triton X-100
-
activates solubilized enzyme
G-3634-A
-
activates solubilized enzyme
-
additional information
-
not activated by Triton X-100
-
additional information
-
CDPcholine stimulates by protecting UDP-GlcNAc from hydrolysis by endogenous enzymes
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.6
-
asialo-alpha1-acid glycoprotein
-
-
-
1
-
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
0.44
-
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
PC-12 cells
0.9
-
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
PC-12D cells
0.36
-
Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
-
recombinant enzyme, pH 7.2, 37C
0.19
-
lactoneotetraosylceramide
-
-
0.19
-
lactonorhexaosylceramide
-
-
-
5.2
-
lactose
-
-
18.2
-
lactose
-
-
2.2
-
N-acetyllactosamine
-
-
7
-
N-acetyllactosamine
-
-
19.6
-
N-acetyllactosamine
-
-
0.129
-
UDP-N-acetyl-D-glucosamine
-
-
0.133
-
UDP-N-acetyl-D-glucosamine
-
lactose as acceptor
0.17
-
UDP-N-acetyl-D-glucosamine
-
lactosylceramide as acceptor
1.445
-
UDP-N-acetyl-D-glucosamine
-
using Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37C
1.487
-
UDP-N-acetyl-D-glucosamine
-
using Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37C
2.082
-
UDP-N-acetyl-D-glucosamine
-
using Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37C
8
-
UDP-N-acetyl-D-glucosamine
-
PC-12 and PC-12D cells
15.97
-
UDP-N-acetyl-D-glucosamine
-
using Galbeta(1->3)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37C
29.8
-
lactose
-
-
additional information
-
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00153
-
-
-
0.118
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
7.5
-
broad
6.5
7
-
-
6.8
7.2
-
asialo-alpha1-acid glycoprotein as acceptor
6.8
7.8
-
broad
7
7.2
-
highest activity, with Hepes buffer
7
-
-
broad pH-optimum around pH 7
additional information
-
-, Q8K0J2
pI: 8.7
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
7.5
-
substantial reaction in the range, 65% of activity at pH 7
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
O43505
assay at
37
-
-
assay at
42
-
-
112% of activity compared with that at 37C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
O43505
highly expressed in fetal and adult brain
Manually annotated by BRENDA team
-, Q8K0J2
very weak expression of beta3GnT7
Manually annotated by BRENDA team
Mus musculus beta3GnT7
-
very weak expression of beta3GnT7
-
Manually annotated by BRENDA team
-, Q8K0J2
very weak expression of beta3GnT7
Manually annotated by BRENDA team
-
Pro-5 and Lec2 CHO cells
Manually annotated by BRENDA team
-, Q8K0J2
beta3GnT7 is most strongly expressed in the placenta and colon
Manually annotated by BRENDA team
-, Q8K0J2
breast cancer cell line, higher expression of beta3GnT7 in cancer cell lines with low degrees of invasiveness
Manually annotated by BRENDA team
-, Q8K0J2
very weak expression of beta3GnT7
Manually annotated by BRENDA team
Mus musculus beta3GnT7
-
very weak expression of beta3GnT7
-
Manually annotated by BRENDA team
-, Q8K0J2
moderate expression of beta3GnT7
Manually annotated by BRENDA team
O43505
highly expressed in fetal kidney
Manually annotated by BRENDA team
Mus musculus beta3GnT7
-
moderate expression of beta3GnT7
-
Manually annotated by BRENDA team
-, Q8K0J2
KLN205-MUC1, lung cancer cell line, higher expression of beta3GnT7 in cancer cell lines with low degrees of invasiveness
Manually annotated by BRENDA team
-, Q8K0J2
moderate expression of beta3GnT7
Manually annotated by BRENDA team
-
pheochromacytoma cells, nerve growth factor stimulated cells with reduced GnT-i activity compared to unstimulated cells
Manually annotated by BRENDA team
-
new subline of pheochromacytoma cells PC-12, low GnT-i activity
Manually annotated by BRENDA team
-, Q8K0J2
beta3GnT7 is most strongly expressed in the placenta at the later stages of gestation and in colon
Manually annotated by BRENDA team
Mus musculus beta3GnT7
-
beta3GnT7 is most strongly expressed in the placenta at the later stages of gestation and in colon
-
Manually annotated by BRENDA team
-
normal blood group 0 serum
Manually annotated by BRENDA team
-
newborn calf serum
Manually annotated by BRENDA team
-, Q8K0J2
moderate expression of beta3GnT7
Manually annotated by BRENDA team
-, Q8K0J2
moderate expression of beta3GnT7
Manually annotated by BRENDA team
Mus musculus beta3GnT7
-
moderate expression of beta3GnT7
-
Manually annotated by BRENDA team
O43505
colonic carcinoma cell line
Manually annotated by BRENDA team
-
colonic adenocarcinoma cell line
Manually annotated by BRENDA team
-, Q8K0J2
very weak expression of beta3GnT7
Manually annotated by BRENDA team
O43505
human melanoma cell line
Manually annotated by BRENDA team
additional information
-
enzyme is undetectable in normal colonic epithelial cells, but is greatly enhanced both during fetal development and in colonic adenocarcinomas
Manually annotated by BRENDA team
additional information
O43505
enzyme is expressed ubiquitously in various adult tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
O43505
stem region and large catalytic domain reside presumably in the Golgi lumen
Manually annotated by BRENDA team
-
B3GNT1 and B4GALT1 colocalize and interact with each other in the trans-Golgi
Manually annotated by BRENDA team
-
membrane-bound
Manually annotated by BRENDA team
O43505
type II membrane protein topology, longest transmembrane domain among glycosyltransferases cloned so far
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
90000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-, Q8K0J2
x * 45000, calculated from the amino acid sequence
?
O43505
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
?
Mus musculus beta3GnT7
-
x * 45000, calculated from the amino acid sequence
-
monomer
-
1 * 70000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
-
glycoprotein
O43505
2 potential N-glycosylation sites
glycoprotein
-
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
-
4% of activity compared with that at 37C
12
-
-
9% of activity compared with that at 37C
20
-
-
28% of activity compared with that at 37C
30
-
-
63% of activity compared with that at 37C
42
-
-
112% of activity compared with that at 37C
45
-
-
88% of activity compared with that at 37C
50
-
-
10% of activity compared with that at 37C
55
-
-
2% of activity compared with that at 37C
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, purified enzyme, at least 2 weeks, stable
-
0-4C, lyophilized enzyme form, over 6 months, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
125000fold
-
partial, 178fold, from Novikoff tumor cell ascites fluid
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells
O43505
expression in COS-7 cells, localization in membranes
-
human ortholog to mouse enzyme beta3GnT7 has the chromosomal locus 2q37.1
Q8NFL0
plasmid cDNA encoding the catalytic domain of i-GlcNAcT, amino acids 53-415, is expressed in COS-1 cells
-
cDNA from placenta encoding beta3GnT7 is cloned and partially characterized, open reading frame encodes a 397-amino acids protein
-, Q8K0J2
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the decrease of laminin-binding glycans and consequent increased cell migration are associated with the decreased expression of beta3-N-acetylglucosaminyltransferase-1
-
forced expression of beta3GnT1 in aggressive cancer cells restores the laminin-binding glycans and decreased tumor formation
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APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-, Q8K0J2
enzyme may be used as diagnostic marker of human cancer or as target molecule for the development of a new therapy
medicine
Mus musculus beta3GnT7
-
enzyme may be used as diagnostic marker of human cancer or as target molecule for the development of a new therapy
-