Information on EC 2.4.1.149 - N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.149
-
RECOMMENDED NAME
GeneOntology No.
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosaminoglycan biosynthesis - keratan sulfate
-
-
Glycosphingolipid biosynthesis - lacto and neolacto series
-
-
Metabolic pathways
-
-
terminal O-glycans residues modification
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine 3-beta-N-acetyl-D-glucosaminyltransferase
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoprotein and other glycoproteins and oligosaccharides.
CAS REGISTRY NUMBER
COMMENTARY hide
85638-39-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
newborn calf
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Mus musculus beta3GnT7
beta3GnT7
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
beta3GnT1 is required for laminin-binding glycan synthesis through formation of a complex with LARGE, thus regulating the function of LARGE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
115% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Fucalpha(1->2)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)[Fucalpha(1->2)]Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
32% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-6)(Galbeta(1-4)GlcNAcbeta(1-2))Manalpha(1-6)Manbeta-octyl
?
show the reaction diagram
-
regioselectivity of enzyme, favored site of GlcNAc addition is the lower beta1,2-branch over the beta1,6-branch by a 3:1 ratio resulting in a mixture of heptasaccharides
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta-p-nitrophenol
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-p-nitrophenol
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
show the reaction diagram
-
76% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-pNP
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-pNP
show the reaction diagram
-
poor acceptor, 23% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
28% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)Glcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)Glcbeta-O(CH2)3N3
show the reaction diagram
-
65% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAc
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
show the reaction diagram
-
73% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
107% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
90% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
57% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
show the reaction diagram
-
101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
show the reaction diagram
-
104% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
100% activity
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
UDP + GlcNAcbeta(1->3)Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
show the reaction diagram
-
26% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
-
i.e. L2L4, 72% activity compared to L2L2
-
-
-
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
-
i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate
-
-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
-
mixture of the pentasaccharide product, 27 mol%, and the unreacted tetrasaccharide, 73 mol%
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OMe
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OR
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OR
show the reaction diagram
-
i.e. N,N-diacetyllactosediamine-OR
only 12.5% of the substrate is converted into the product, which is unusually resistant towards jackbean beta-N-acetylhexosaminidase
?
UDP-N-acetyl-D-glucosamine + glycolipid
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + O-glycan
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
poly-N-acetyllactosamine is biosynthesized by the alternating addition of N-acetyl-D-glucosamine by UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase and Gal by UDP-Gal:betaGlcNAc beta-1,4-galactosyltransferase
-
-
?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
UDP-N-acetylglucosamine + asialo-fetuin
UDP + N-acetylglucosaminylated asialo-fetuin
show the reaction diagram
-
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
-
-
?
UDP-N-acetylglucosamine + asialo-transferrin
UDP + N-acetylglucosaminylated asialo-transferrin
show the reaction diagram
-
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
-
-
?
UDP-N-acetylglucosamine + lactoneotetraosylceramide
?
show the reaction diagram
-
lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length
-
-
?
UDP-N-acetylglucosamine + lactonorhexaosylceramide
?
show the reaction diagram
-
lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length
-
-
?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
UDP-N-acetylglucosamine + lactosylceramide
?
show the reaction diagram
-
lactosylceramide from human erythrocytes
-
-
?
UDP-N-acetylglucosamine + lactotetraosylceramide
?
show the reaction diagram
-
lactotetraosylceramide from human meconium, poor substrate
-
-
?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + glycolipid
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + O-glycan
?
show the reaction diagram
-
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
about one third of the activity compared to Mn2+, recombinant enzyme
Cd2+
-
CdCl2 slightly stimulates
Co2+
-
slightly stimulates, 12.8% as effective as Mn2+ at 10 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
4-Thiouridine diphosphate
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inhibits in a concentration-dependent manner
CH3CN
-
-
N-acetyllactosamine
-
strong product inhibition
SDS
-
-
UDP
-
1 mM, 70% inhibition
additional information
-
nerve growth factor stimulated PC-12 cells with reduced GnT-i activity compared to unstimulated cells, no effect in PC-12D cells
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
omitting ATP from reaction mixture decreases activity by 87%
CHAPSO
-
activates solubilized enzyme
deoxycholate
-
activates solubilized enzyme
G-3634-A
-
activates solubilized enzyme
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Triton CF-54
-
activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3%
Triton X-100
-
activates solubilized enzyme
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
asialo-alpha1-acid glycoprotein
-
-
-
1
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
0.44 - 0.9
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
0.36
Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
-
recombinant enzyme, pH 7.2, 37C
0.19
lactoneotetraosylceramide
5.2 - 29.8
lactose
2.2 - 19.6
N-acetyllactosamine
0.129 - 15.97
UDP-N-acetyl-D-glucosamine
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.118
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 7.5
-
broad
6.5 - 7
-
-
6.8 - 7.8
-
broad
6.8 - 7.2
-
asialo-alpha1-acid glycoprotein as acceptor
7
-
broad pH-optimum around pH 7
7 - 7.2
-
highest activity, with Hepes buffer
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
substantial reaction in the range, 65% of activity at pH 7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
-
112% of activity compared with that at 37C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highly expressed in fetal and adult brain
Manually annotated by BRENDA team
very weak expression of beta3GnT7
Manually annotated by BRENDA team
-
Pro-5 and Lec2 CHO cells
Manually annotated by BRENDA team
beta3GnT7 is most strongly expressed in the placenta and colon
Manually annotated by BRENDA team
breast cancer cell line, higher expression of beta3GnT7 in cancer cell lines with low degrees of invasiveness
Manually annotated by BRENDA team
KLN205-MUC1, lung cancer cell line, higher expression of beta3GnT7 in cancer cell lines with low degrees of invasiveness
Manually annotated by BRENDA team
moderate expression of beta3GnT7
Manually annotated by BRENDA team
-
pheochromacytoma cells, nerve growth factor stimulated cells with reduced GnT-i activity compared to unstimulated cells
Manually annotated by BRENDA team
-
new subline of pheochromacytoma cells PC-12, low GnT-i activity
Manually annotated by BRENDA team
moderate expression of beta3GnT7
Manually annotated by BRENDA team
colonic carcinoma cell line
Manually annotated by BRENDA team
-
colonic adenocarcinoma cell line
Manually annotated by BRENDA team
very weak expression of beta3GnT7
Manually annotated by BRENDA team
human melanoma cell line
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 70000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
4% of activity compared with that at 37C
12
-
9% of activity compared with that at 37C
20
-
28% of activity compared with that at 37C
30
-
63% of activity compared with that at 37C
42
-
112% of activity compared with that at 37C
45
-
88% of activity compared with that at 37C
50
-
10% of activity compared with that at 37C
55
-
2% of activity compared with that at 37C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4C, lyophilized enzyme form, over 6 months, stable
-
4C, purified enzyme, at least 2 weeks, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
125000fold
-
partial, 178fold, from Novikoff tumor cell ascites fluid
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells
cDNA from placenta encoding beta3GnT7 is cloned and partially characterized, open reading frame encodes a 397-amino acids protein
expression in COS-7 cells, localization in membranes
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human ortholog to mouse enzyme beta3GnT7 has the chromosomal locus 2q37.1
plasmid cDNA encoding the catalytic domain of i-GlcNAcT, amino acids 53-415, is expressed in COS-1 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
forced expression of beta3GnT1 in aggressive cancer cells restores the laminin-binding glycans and decreased tumor formation
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the decrease of laminin-binding glycans and consequent increased cell migration are associated with the decreased expression of beta3-N-acetylglucosaminyltransferase-1
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine