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Literature summary for 2.4.1.149 extracted from

  • Peng, W.; Pranskevich, J.; Nycholat, C.; Gilbert, M.; Wakarchuk, W.; Paulson, J.C.; Razi, N.
    Helicobacter pylori beta1,3-N-acetylglucosaminyltransferase for versatile synthesis of type 1 and type 2 poly-LacNAcs on N-linked, O-linked and I-antigen glycans (2012), Glycobiology, 22, 1453-1464.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.149

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.445
-
 UDP-N-acetyl-D-glucosamine using Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C Helicobacter pylori
1.487
-
 UDP-N-acetyl-D-glucosamine using Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C Helicobacter pylori
2.082
-
 UDP-N-acetyl-D-glucosamine using Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C Helicobacter pylori
15.97
-
 UDP-N-acetyl-D-glucosamine using Galbeta(1->3)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H 115% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
-
 ?
additional information the enzyme shows no activity on a terminal Gal with Neu5Acalpha(2->6) substitution. Terminal galactose with alpha-linkage, Galalpha(1->3)Gal or Galalpha(1->4)Gal is not an acceptor substrate for the enzyme Helicobacter pylori ?
-
 ?
UDP-N-acetyl-D-glucosamine + Fucalpha(1->2)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 32% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)[Fucalpha(1->2)]Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H 48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-O(CH2)2NH2 76% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-O(CH2)2NH2
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GalNAcbeta-pNP poor acceptor, 23% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->3)GalNAcbeta-pNP
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)GlcNAcbeta-O(CH2)3N3 48% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->3)GlcNAcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H 101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->3)[Galbeta(1->4)GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H 28% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->3)[GlcNAcbeta(1->6)]GalNAcalpha-OCH(CH3)CH(NH2)CO2H
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)Glcbeta-O(CH2)3N3 65% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)Glcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAc 73% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 107% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 90% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H 57% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H 101% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)GalNAcalpha-OCH(CH3)CH(NH2)CO2H
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP 104% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->6)[GlcNAcbeta(1->4)]Galbeta(1->4)GlcNAcbeta-pNP
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 100% activity Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta-O(CH2)3N3
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3 26% activity compared to Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 Helicobacter pylori UDP + GlcNAcbeta(1->3)Galbeta(1->4)[Fucalpha(1->3)]GlcNAcbeta-O(CH2)3N3
-
 ?

Synonyms

Synonyms Comment Organism
beta1,3-N-acetylglucosaminyltransferase
-
 Helicobacter pylori
beta3GlcNAcT
-
 Helicobacter pylori
JHP1032
-
 Helicobacter pylori