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Literature summary for 2.4.1.149 extracted from

  • Holmes, E.H.
    Characterization of a beta 1-->3-N-acetylglucosaminyltransferase associated with synthesis of type 1 and type 2 lacto-series tumor-associated antigens from the human colonic adenocarcinoma cell line SW403 (1988), Arch. Biochem. Biophys., 260, 461-468.
    View publication on PubMed
Search for more literature for 2.4.1.149

Activating Compound

Activating Compound Comment Organism Structure
CHAPSO activates solubilized enzyme Homo sapiens
deoxycholate activates solubilized enzyme Homo sapiens
G-3634-A activates solubilized enzyme Homo sapiens
additional information CDPcholine stimulates by protecting UDP-GlcNAc from hydrolysis by endogenous enzymes Homo sapiens
Triton CF-54 activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3% Homo sapiens
Triton X-100 activates solubilized enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 UDP-N-acetyl-D-glucosamine lactosylceramide as acceptor Homo sapiens
0.19
-
 lactoneotetraosylceramide
-
 Homo sapiens
0.19
-
 lactonorhexaosylceramide
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ strict requirement Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme is undetectable in normal colonic epithelial cells, but is greatly enhanced both during fetal development and in colonic adenocarcinomas Homo sapiens
-
SW-403 cell colonic adenocarcinoma cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information transfer of GlcNAc occurs mainly to type 2 chain nonfucosylated structures, elongation of type 1 chain structure Lc4 is also detectable, no transfer to any fucosylated derivative of either type 1 or 2 chains, transfer of GlcNAc to a terminal Gal residue on a lacto-series core chain Homo sapiens ?
-
 ?
additional information biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia Homo sapiens ?
-
 ?
UDP-N-acetylglucosamine + lactoneotetraosylceramide lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length Homo sapiens ?
-
 ?
UDP-N-acetylglucosamine + lactonorhexaosylceramide lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length Homo sapiens ?
-
 ?
UDP-N-acetylglucosamine + lactosylceramide lactosylceramide from human erythrocytes Homo sapiens ?
-
 ?
UDP-N-acetylglucosamine + lactotetraosylceramide lactotetraosylceramide from human meconium, poor substrate Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 7.5 broad Homo sapiens
7 7.2 highest activity, with Hepes buffer Homo sapiens