Cloned (Comment) | Organism |
---|---|
plasmid cDNA encoding the catalytic domain of i-GlcNAcT, amino acids 53-415, is expressed in COS-1 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + N-glycan | Homo sapiens | polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | Homo sapiens | poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-6)(Galbeta(1-4)GlcNAcbeta(1-2))Manalpha(1-6)Manbeta-octyl | regioselectivity of enzyme, favored site of GlcNAc addition is the lower beta1,2-branch over the beta1,6-branch by a 3:1 ratio resulting in a mixture of heptasaccharides | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta-p-nitrophenol | - |
Homo sapiens | UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-p-nitrophenol | - |
? | |
UDP-N-acetyl-D-glucosamine + N-glycan | polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |