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Literature summary for 2.4.1.149 extracted from

  • Sasaki, K.; Kurata-Miura, K.; Ujita, M.; Angata, K.; Nakagawa, S.; Sekine, S.; Nishi, T.; Fukuda, M.
    Expression cloning of cDNA encoding a human beta-1,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis (1997), Proc. Natl. Acad. Sci. USA, 94, 14294-14299.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.149

Cloned(Commentary)

Cloned (Comment) Organism
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus stem region and large catalytic domain reside presumably in the Golgi lumen Homo sapiens 5794
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membrane type II membrane protein topology, longest transmembrane domain among glycosyltransferases cloned so far Homo sapiens 16020
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47125
-
 x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R Homo sapiens enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43505
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2 potential N-glycosylation sites Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain highly expressed in fetal and adult brain Homo sapiens
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kidney highly expressed in fetal kidney Homo sapiens
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additional information enzyme is expressed ubiquitously in various adult tissues Homo sapiens
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SW-1116 cell colonic carcinoma cell line Homo sapiens
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WM-266-4 cell human melanoma cell line Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 Homo sapiens UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
 Homo sapiens ?
-
 ?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
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 Homo sapiens UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?

Subunits

Subunits Comment Organism
? x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens