Information on EC 1.14.11.16 - peptide-aspartate beta-dioxygenase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
1.14.11.16
-
RECOMMENDED NAME
GeneOntology No.
peptide-aspartate beta-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
peptide-L-aspartate + 2-oxoglutarate + O2 = peptide-3-hydroxy-L-aspartate + succinate + CO2
show the reaction diagram
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
peptide-L-aspartate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Requires Fe2+. Some vitamin K-dependent coagulation factors, as well as synthetic peptides based on the structure of the first epidermal growth factor domain of human coagulation factor IX or X, can act as acceptors.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aspartate beta-hydroxylase
-
-
-
-
aspartyl (asparaginyl) beta-hydroxylase
-
-
aspartyl (asparaginyl)-beta-hydroxylase
-
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aspartyl beta-hydroxylase
-
-
aspartyl-(asparaginyl) beta-hydroxylase
-
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aspartyl-(asparaginyl)-b-hydroxylase
-
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aspartyl-(asparaginyl)-beta-hydroxylase
-
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aspartyl-(asparagyl)-beta-hydroxylase
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aspartyl-asparaginyl-beta-hydroxylase
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aspartylpeptide beta-dioxygenase
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-
-
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EGF beta-hydroxylase
-
-
EGFH
-
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factor inhibiting HIF
-
-
factor inhibiting hypoxia-inducible factor
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factor inhibiting hypoxia-inducible transcription factor (HIF)
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factor-inhibiting hypoxia-inducible factor
-
-
HIF asparaginyl hydroxylase
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human aspartyl (asparaginyl) beta-hydroxylase
-
-
hypoxia-inducible factor asparaginyl hydroxylase
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-
CAS REGISTRY NUMBER
COMMENTARY
122544-66-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
human osteosarcoma
-
-
Manually annotated by BRENDA team
three different size transcripts 2.8, 4.5 and 6.6 kb, the bigger ones lead to active proteins after expression
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-
Manually annotated by BRENDA team
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0167
-
first epidermal growth factor-like domain
-
substrate is of human protein S, 56 kDa protein
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0.0217
-
first epidermal growth factor-like domain
-
substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein
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0.025
-
first epidermal growth factor-like domain
-
substrate is of human protein S, 52 kDa protein
-
0.0317
-
first epidermal growth factor-like domain
-
substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.59
-
-
-
14
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
7.9
-
about half-maximal activity at pH 6.5 and 7.9
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, 50 mM Tris-HCl pH 7.2, 1 mg/ml bovine serum albumin, at least 8 weeks stable
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C637A
-
38% activity of wild-type
C644A
-
62% activity of wild-type
C656A
-
100% activity of wild-type
C681A
-
60% activity of wild-type
C696A
-
29% activity of wild-type
G659A
-
21% activity of wild-type
G669A
-
90% activity of wild-type
H667L
-
106% activity of wild-type
H671L
-
16% activity of wild-type
H675D
-
20% activity of wild-type
H675E
-
12% activity of wild-type
H686L
-
9% activity of wild-type
P678V
-
90% activity of wild-type
R682A
-
10% activity of wild-type
R684A
-
8% activity of wild-type
R684K
-
87% activity of wild-type