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Literature summary for 1.14.11.16 extracted from

  • McGinnis, K.; Ku, G.M.; VanDusen, W.J.; Fu, J.; Garsky, V.; Stern, A.M.; Friedman, P.A.
    Site-directed mutagenesis of residues in a conserved region of bovine aspartyl (asparaginyl) beta-hydroxylase: Evidence that histidine 675 has a role in binding Fe2+ (1996), Biochemistry, 35, 3957-3962.
    View publication on PubMed
Search for more literature for 1.14.11.16

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants expressed in Escherichia coli BL21 Bos taurus

Protein Variants

Protein Variants Comment Organism
G659A 21% activity of wild-type Bos taurus
G669A 90% activity of wild-type Bos taurus
H667L 106% activity of wild-type Bos taurus
H671L 16% activity of wild-type Bos taurus
H675D 20% activity of wild-type Bos taurus
H675E 12% activity of wild-type Bos taurus
H686L 9% activity of wild-type Bos taurus
P678V 90% activity of wild-type Bos taurus
R682A 10% activity of wild-type Bos taurus
R684A 8% activity of wild-type Bos taurus
R684K 87% activity of wild-type Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 Fe2+ at 0.6 mM 2-oxoglutarate, wild-type Bos taurus
0.052
-
 Fe2+ at 0.6 mM 2-oxoglutarate, H675E mutant Bos taurus
0.093
-
 Fe2+ at 0.6 mM 2-oxoglutarate, H675D mutant Bos taurus
0.098
-
 2-oxoglutarate at 1 mM Fe2+, H675D mutant Bos taurus
0.102
-
 2-oxoglutarate at 1 mM Fe2+, wild-type Bos taurus
0.125
-
 2-oxoglutarate at 1 mM Fe2+, H675E mutant Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptide L-aspartate + 2-oxoglutarate + O2 second epidermal growth factor-like domain of bovine protein S Bos taurus peptide 3-hydroxy-L-aspartate + succinate + CO2
-
 ?