Information on EC 1.1.1.83 - D-malate dehydrogenase (decarboxylating)

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
1.1.1.83
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RECOMMENDED NAME
GeneOntology No.
D-malate dehydrogenase (decarboxylating)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(R)-malate + NAD+ = pyruvate + CO2 + NADH
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
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oxidative decarboxylation
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redox reaction
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reduction
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PATHWAY
KEGG Link
MetaCyc Link
Butanoate metabolism
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D-malate degradation
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SYSTEMATIC NAME
IUBMB Comments
(R)-malate:NAD+ oxidoreductase (decarboxylating)
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-malate dehydrogenase
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D-malic enzyme
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dehydrogenase, D-malate (decarboxylating)
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dmlA
P76251
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CAS REGISTRY NUMBER
COMMENTARY
37250-20-7
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Pseudomonas fluorescens UK-1
UK-1
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Manually annotated by BRENDA team
bifunctional L(+)-tartrate dehydrogenase-D(+)-malate dehydrogenase,decarboxylating, EC 1.1.1.93/EC 1.1.1.83
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Manually annotated by BRENDA team
Rhodobacter sphaeroides Y
strain Y
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Manually annotated by BRENDA team
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
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induced by D-malate
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EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
addition of nitrate during anaerobic growth represses the expression of dmlA-lacZ about 2.2fold, but the expression is still higher than the expression under aerobic conditions. The presence of glucose during anaerobic growth represses dmlA expression to levels similar to those observed after nitrate addition, suggesting that there is some glucose repression (2.4fold)
P76251
in a wild-type background, D-malate and meso- and L-tartrate cause high levels of induction of dmlA-lacZ expression (up to 12.3fold). With L-malate, succinate, and D-tartrate there is only weak induction. Induction of dmlA encoding DmlA requires an intact dmlR gene, which encodes DmlR, a LysR-type transcriptional regulator; the expression of dmlA-lacZ at high levels is induced under anaerobic conditions in the presence of D-malate and is more than 5fold greater than the expression under aerobic conditions
P76251
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
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sequential fluorometric quantification of malic acid enantiomers in a single line flow-injection system using immobilized-enzyme reactors. An immobilized D-malate dehydrogenase (EC 1.1.1.83) reactor and an immobilized L-malate dehydrogenase (EC 1.1.1.40) reactor are introduced into the flowline in series. Sample and coenzyme (NAD+ or NADP+) are injected into the flow line by an open sandwich method. D-Malate is selectively oxidized by EC 1.1.1.83 when NAD+ is injected with a sample. When NADP+ is injected with a sample, L -malate is oxidized only by 1.1.1.40. NADH or NADPH produced by the immobilized-enzyme reactors is monitored fluorometrically at 455 nm