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show all sequences of 1.1.1.83

Purification and characterization of a bifunctional L-(+)-tartrate dehydrogenase-D-(+)-malate dehydrogenase (decarboxylating) from Rhodopseudomonas sphaeroides Y

Giffhorn, F.; Kuhn, A.; J. Bacteriol. 155, 281-290 (1983)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NH4+
optimal activity in presence of both Mn2+ and NH4+
Rhodobacter sphaeroides
General Stability
General Stability
Organism
10-40% loss of activity during repeated freezing and thawing
Rhodobacter sphaeroides
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
-
Rhodobacter sphaeroides
Dihydroxyfumarate
noncompetitive
Rhodobacter sphaeroides
iodoacetate
-
Rhodobacter sphaeroides
L-Tartrate
competitive with respect to D-malate
Rhodobacter sphaeroides
meso-tartrate
competitive
Rhodobacter sphaeroides
oxaloacetate
competitive
Rhodobacter sphaeroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
NAD+
-
Rhodobacter sphaeroides
0.17
-
D-malate
-
Rhodobacter sphaeroides
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
1 mM, 30% of the activity with Mn2+
Rhodobacter sphaeroides
K2SO4
50 mM, 56% of the activity with Mn2+
Rhodobacter sphaeroides
KCl
50 mM, 63% of the activity with Mn2+
Rhodobacter sphaeroides
Mg2+
5 mM, 59% of the activity with Mn2+
Rhodobacter sphaeroides
Mn2+
Km: 0.016 mM, optimal activity in presence of both Mn2+ and NH4+
Rhodobacter sphaeroides
NaCl
50 mM, 19% of the activity with Mn2+
Rhodobacter sphaeroides
Zn2+
0.1 mM, 14% of the activity with Mn2+
Rhodobacter sphaeroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
4 * 38500, SDS-PAGE
Rhodobacter sphaeroides
158000
-
gel filtration
Rhodobacter sphaeroides
162000
-
equilibrium sedimentation
Rhodobacter sphaeroides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
-
strain Y
-
Rhodobacter sphaeroides Y
-
strain Y
-
Purification (Commentary)
Commentary
Organism
bifunctional L-(+)-tartrate-D-(+)-malate dehydrogenase
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
20.6
-
-
Rhodobacter sphaeroides
Storage Stability
Storage Stability
Organism
-20C, stable for months
Rhodobacter sphaeroides
0C, stable for 30 h, even at protein concentration below 1 mg/ml
Rhodobacter sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-malate + NAD+
-
389609
Rhodobacter sphaeroides
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
389609
Rhodobacter sphaeroides Y
pyruvate + CO2 + NADH
-
-
-
?
additional information
bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate
389609
Rhodobacter sphaeroides
?
-
-
-
-
additional information
bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate
389609
Rhodobacter sphaeroides Y
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 38500, SDS-PAGE
Rhodobacter sphaeroides
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Rhodobacter sphaeroides
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
half-life: 30 h
Rhodobacter sphaeroides
50
-
half-life: 120 min
Rhodobacter sphaeroides
55
-
half-life: 30 min
Rhodobacter sphaeroides
60
-
half-life: 9 min
Rhodobacter sphaeroides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
9
-
Rhodobacter sphaeroides
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10.6
at pH 7.0 and 10.6: about 50% of activity maximum
Rhodobacter sphaeroides
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
-
30C, 4 h, stable
Rhodobacter sphaeroides
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhodobacter sphaeroides
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NH4+
optimal activity in presence of both Mn2+ and NH4+
Rhodobacter sphaeroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhodobacter sphaeroides
General Stability (protein specific)
General Stability
Organism
10-40% loss of activity during repeated freezing and thawing
Rhodobacter sphaeroides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
-
Rhodobacter sphaeroides
Dihydroxyfumarate
noncompetitive
Rhodobacter sphaeroides
iodoacetate
-
Rhodobacter sphaeroides
L-Tartrate
competitive with respect to D-malate
Rhodobacter sphaeroides
meso-tartrate
competitive
Rhodobacter sphaeroides
oxaloacetate
competitive
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
NAD+
-
Rhodobacter sphaeroides
0.17
-
D-malate
-
Rhodobacter sphaeroides
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
1 mM, 30% of the activity with Mn2+
Rhodobacter sphaeroides
K2SO4
50 mM, 56% of the activity with Mn2+
Rhodobacter sphaeroides
KCl
50 mM, 63% of the activity with Mn2+
Rhodobacter sphaeroides
Mg2+
5 mM, 59% of the activity with Mn2+
Rhodobacter sphaeroides
Mn2+
Km: 0.016 mM, optimal activity in presence of both Mn2+ and NH4+
Rhodobacter sphaeroides
NaCl
50 mM, 19% of the activity with Mn2+
Rhodobacter sphaeroides
Zn2+
0.1 mM, 14% of the activity with Mn2+
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
4 * 38500, SDS-PAGE
Rhodobacter sphaeroides
158000
-
gel filtration
Rhodobacter sphaeroides
162000
-
equilibrium sedimentation
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
Commentary
Organism
bifunctional L-(+)-tartrate-D-(+)-malate dehydrogenase
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
20.6
-
-
Rhodobacter sphaeroides
Storage Stability (protein specific)
Storage Stability
Organism
-20C, stable for months
Rhodobacter sphaeroides
0C, stable for 30 h, even at protein concentration below 1 mg/ml
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-malate + NAD+
-
389609
Rhodobacter sphaeroides
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
389609
Rhodobacter sphaeroides Y
pyruvate + CO2 + NADH
-
-
-
?
additional information
bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate
389609
Rhodobacter sphaeroides
?
-
-
-
-
additional information
bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate
389609
Rhodobacter sphaeroides Y
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 38500, SDS-PAGE
Rhodobacter sphaeroides
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Rhodobacter sphaeroides
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
half-life: 30 h
Rhodobacter sphaeroides
50
-
half-life: 120 min
Rhodobacter sphaeroides
55
-
half-life: 30 min
Rhodobacter sphaeroides
60
-
half-life: 9 min
Rhodobacter sphaeroides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
9
-
Rhodobacter sphaeroides
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10.6
at pH 7.0 and 10.6: about 50% of activity maximum
Rhodobacter sphaeroides
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
-
30C, 4 h, stable
Rhodobacter sphaeroides
Other publictions for EC 1.1.1.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712297
Lukas
Regulation of aerobic and anae ...
Escherichia coli
J. Bacteriol.
192
2503-2511
2010
-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
2
-
-
2
-
-
670917
Tsukatani
Sequential fluorometric quanti ...
Escherichia coli
Talanta
65
396-401
2005
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
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-
-
-
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-
-
-
-
-
-
-
287429
Ebbighausen
-
A novel mechanism involved in ...
Rhodobacter sphaeroides
Arch. Microbiol.
138
338-344
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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-
-
-
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2
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-
-
1
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-
1
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389609
Giffhorn
Purification and characterizat ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides Y
J. Bacteriol.
155
281-290
1983
1
-
-
-
-
1
6
2
-
7
3
-
-
6
-
-
1
-
-
-
1
2
4
1
1
-
4
-
1
1
1
1
-
-
-
1
-
-
1
-
-
1
-
6
-
2
-
7
3
-
-
-
-
1
-
-
1
2
4
1
1
-
4
-
1
1
1
-
-
-
-
-
-
-
287433
Knichel
D-Malic enzyme of Pseudomonas ...
Enterobacter aerogenes, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida
Eur. J. Biochem.
123
547-552
1982
-
-
-
-
-
-
10
2
-
2
1
-
-
11
-
-
1
-
-
-
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
2
1
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
207978
Lhdesmki
Comparison of D-malate and bet ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Biochim. Biophys. Acta
613
266-274
1980
-
-
-
-
-
-
3
2
-
-
2
-
-
5
-
-
1
-
-
-
1
1
2
1
-
-
2
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
2
-
-
-
-
1
-
-
1
1
2
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287434
Laehdesmaeki
D-Malate dehydrogenase from ps ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Acta Chem. Scand. B
34
423-427
1980
-
-
-
-
-
1
2
-
-
-
2
-
-
6
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
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-
-
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287431
Stern
Oxidation D-malic and beta-alk ...
Enterobacter aerogenes, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
98
147-151
1969
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
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-
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