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Literature summary for 1.1.1.83 extracted from
- D-Malic enzyme of Pseudomonas fluorescens (1982), Eur. J. Biochem., 123, 547-552.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Pseudomonas fluorescens |  |
| AMP | - |
Pseudomonas fluorescens | |
| ATP | - |
Pseudomonas fluorescens | |
| ATP | - |
Pseudomonas putida | |
| CoA | - |
Pseudomonas fluorescens | |
| D-lactate | - |
Pseudomonas fluorescens | |
| D-lactate | - |
Pseudomonas putida | |
| L-lactate | - |
Pseudomonas fluorescens | |
| meso-tartrate | - |
Pseudomonas fluorescens | |
| oxaloacetate | - |
Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
NAD+ | - |
Pseudomonas fluorescens | |
| 0.3 | - |
(R)-malate | - |
Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required | Pseudomonas fluorescens | |
| Mn2+ | Mn2+ or Mg2+ required | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 175000 | - |
gel filtration, gradient gel electrophoresis | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-malate + NAD+ | - |
Pseudomonas fluorescens | pyruvate + CO2 + NADH | - |
? | |
| (R)-malate + NAD+ | - |
Klebsiella aerogenes | pyruvate + CO2 + NADH | - |
? | |
| (R)-malate + NAD+ | - |
Pseudomonas aeruginosa | pyruvate + CO2 + NADH | - |
? | |
| (R)-malate + NAD+ | - |
Pseudomonas putida | pyruvate + CO2 + NADH | - |
? | |
| (R)-malate + NAD+ | - |
Pseudomonas fluorescens | pyruvate + CO2 + NADH + H+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | 60 | - |
Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 8.8 | - |
Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas fluorescens | |
| NAD+ | - |
Pseudomonas putida | |
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Release 2023.1 (January 2023)
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