BRENDA - Enzyme Database show
show all sequences of 1.1.1.83

D-Malic enzyme of Pseudomonas fluorescens

Knichel, W.; Radler, F.; Eur. J. Biochem. 123, 547-552 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Pseudomonas fluorescens
AMP
-
Pseudomonas fluorescens
ATP
-
Pseudomonas fluorescens
ATP
-
Pseudomonas putida
CoA
-
Pseudomonas fluorescens
D-lactate
-
Pseudomonas fluorescens
D-lactate
-
Pseudomonas putida
L-lactate
-
Pseudomonas fluorescens
meso-tartrate
-
Pseudomonas fluorescens
oxaloacetate
-
Pseudomonas fluorescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
NAD+
-
Pseudomonas fluorescens
0.3
-
malate
-
Pseudomonas fluorescens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
Mn2+ or Mg2+ required
Pseudomonas fluorescens
Mn2+
Mn2+ or Mg2+ required
Pseudomonas fluorescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
175000
-
gel filtration, gradient gel electrophoresis
Pseudomonas fluorescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterobacter aerogenes
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-malate + NAD+
-
287433
Pseudomonas fluorescens
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
287433
Enterobacter aerogenes
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
287433
Pseudomonas aeruginosa
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
287433
Pseudomonas putida
pyruvate + CO2 + NADH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
57
60
-
Pseudomonas fluorescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
8.8
-
Pseudomonas fluorescens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NAD+
-
Pseudomonas fluorescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NAD+
-
Pseudomonas fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Pseudomonas fluorescens
AMP
-
Pseudomonas fluorescens
ATP
-
Pseudomonas fluorescens
ATP
-
Pseudomonas putida
CoA
-
Pseudomonas fluorescens
D-lactate
-
Pseudomonas fluorescens
D-lactate
-
Pseudomonas putida
L-lactate
-
Pseudomonas fluorescens
meso-tartrate
-
Pseudomonas fluorescens
oxaloacetate
-
Pseudomonas fluorescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
NAD+
-
Pseudomonas fluorescens
0.3
-
malate
-
Pseudomonas fluorescens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
Mn2+ or Mg2+ required
Pseudomonas fluorescens
Mn2+
Mn2+ or Mg2+ required
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
175000
-
gel filtration, gradient gel electrophoresis
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-malate + NAD+
-
287433
Pseudomonas fluorescens
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
287433
Enterobacter aerogenes
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
287433
Pseudomonas aeruginosa
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
287433
Pseudomonas putida
pyruvate + CO2 + NADH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
57
60
-
Pseudomonas fluorescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
8.8
-
Pseudomonas fluorescens
Other publictions for EC 1.1.1.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712297
Lukas
Regulation of aerobic and anae ...
Escherichia coli
J. Bacteriol.
192
2503-2511
2010
-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
2
-
-
2
-
-
670917
Tsukatani
Sequential fluorometric quanti ...
Escherichia coli
Talanta
65
396-401
2005
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287429
Ebbighausen
-
A novel mechanism involved in ...
Rhodobacter sphaeroides
Arch. Microbiol.
138
338-344
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389609
Giffhorn
Purification and characterizat ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides Y
J. Bacteriol.
155
281-290
1983
1
-
-
-
-
1
6
2
-
7
3
-
-
6
-
-
1
-
-
-
1
2
4
1
1
-
4
-
1
1
1
1
-
-
-
1
-
-
1
-
-
1
-
6
-
2
-
7
3
-
-
-
-
1
-
-
1
2
4
1
1
-
4
-
1
1
1
-
-
-
-
-
-
-
287433
Knichel
D-Malic enzyme of Pseudomonas ...
Enterobacter aerogenes, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida
Eur. J. Biochem.
123
547-552
1982
-
-
-
-
-
-
10
2
-
2
1
-
-
11
-
-
1
-
-
-
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
2
1
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
207978
Lähdesmäki
Comparison of D-malate and bet ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Biochim. Biophys. Acta
613
266-274
1980
-
-
-
-
-
-
3
2
-
-
2
-
-
5
-
-
1
-
-
-
1
1
2
1
-
-
2
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
2
-
-
-
-
1
-
-
1
1
2
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287434
Laehdesmaeki
D-Malate dehydrogenase from ps ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Acta Chem. Scand. B
34
423-427
1980
-
-
-
-
-
1
2
-
-
-
2
-
-
6
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287431
Stern
Oxidation D-malic and beta-alk ...
Enterobacter aerogenes, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
98
147-151
1969
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-