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show all sequences of 1.1.1.83

Comparison of D-malate and beta, beta-dimethylmalate dehydrogenases from Pseudomonas fluorescens UK-1

Lähdesmäki, M.; Mäntsälä, P.; Biochim. Biophys. Acta 613, 266-274 (1980)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-Butanedione
-
Pseudomonas fluorescens
N-ethylmaleimide
1 mM, 18% inactivation
Pseudomonas fluorescens
p-chloromercuribenzoate
0.2 mM, 10% inactivation
Pseudomonas fluorescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.49
-
NAD+
-
Pseudomonas fluorescens
2.2
-
D-malate
-
Pseudomonas fluorescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
4 * 34000, SDS-PAGE
Pseudomonas fluorescens
140000
-
gel filtration
Pseudomonas fluorescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas fluorescens
-
UK-1
-
Pseudomonas fluorescens UK-1
-
UK-1
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.02
-
-
Pseudomonas fluorescens
Storage Stability
Storage Stability
Organism
-70°C, pH 7.2, 1 mM EDTA
Pseudomonas fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-malate + NAD+
-
207978
Pseudomonas fluorescens
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
207978
Pseudomonas fluorescens UK-1
pyruvate + CO2 + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 34000, SDS-PAGE
Pseudomonas fluorescens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
NAD+ provides better protection against inactivation than D-malate or beta,beta-dimethyl-DL-malate
Pseudomonas fluorescens
65
-
2 min, 64% loss of activity
Pseudomonas fluorescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
-
Pseudomonas fluorescens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas fluorescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-Butanedione
-
Pseudomonas fluorescens
N-ethylmaleimide
1 mM, 18% inactivation
Pseudomonas fluorescens
p-chloromercuribenzoate
0.2 mM, 10% inactivation
Pseudomonas fluorescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.49
-
NAD+
-
Pseudomonas fluorescens
2.2
-
D-malate
-
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
4 * 34000, SDS-PAGE
Pseudomonas fluorescens
140000
-
gel filtration
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.02
-
-
Pseudomonas fluorescens
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, pH 7.2, 1 mM EDTA
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-malate + NAD+
-
207978
Pseudomonas fluorescens
pyruvate + CO2 + NADH
-
-
-
?
(R)-malate + NAD+
-
207978
Pseudomonas fluorescens UK-1
pyruvate + CO2 + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 34000, SDS-PAGE
Pseudomonas fluorescens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
NAD+ provides better protection against inactivation than D-malate or beta,beta-dimethyl-DL-malate
Pseudomonas fluorescens
65
-
2 min, 64% loss of activity
Pseudomonas fluorescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
-
Pseudomonas fluorescens
Other publictions for EC 1.1.1.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712297
Lukas
Regulation of aerobic and anae ...
Escherichia coli
J. Bacteriol.
192
2503-2511
2010
-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
2
-
-
2
-
-
670917
Tsukatani
Sequential fluorometric quanti ...
Escherichia coli
Talanta
65
396-401
2005
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
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-
287429
Ebbighausen
-
A novel mechanism involved in ...
Rhodobacter sphaeroides
Arch. Microbiol.
138
338-344
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389609
Giffhorn
Purification and characterizat ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides Y
J. Bacteriol.
155
281-290
1983
1
-
-
-
-
1
6
2
-
7
3
-
-
6
-
-
1
-
-
-
1
2
4
1
1
-
4
-
1
1
1
1
-
-
-
1
-
-
1
-
-
1
-
6
-
2
-
7
3
-
-
-
-
1
-
-
1
2
4
1
1
-
4
-
1
1
1
-
-
-
-
-
-
-
287433
Knichel
D-Malic enzyme of Pseudomonas ...
Enterobacter aerogenes, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida
Eur. J. Biochem.
123
547-552
1982
-
-
-
-
-
-
10
2
-
2
1
-
-
11
-
-
1
-
-
-
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
2
1
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
207978
Lähdesmäki
Comparison of D-malate and bet ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Biochim. Biophys. Acta
613
266-274
1980
-
-
-
-
-
-
3
2
-
-
2
-
-
5
-
-
1
-
-
-
1
1
2
1
-
-
2
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
2
-
-
-
-
1
-
-
1
1
2
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287434
Laehdesmaeki
D-Malate dehydrogenase from ps ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Acta Chem. Scand. B
34
423-427
1980
-
-
-
-
-
1
2
-
-
-
2
-
-
6
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287431
Stern
Oxidation D-malic and beta-alk ...
Enterobacter aerogenes, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
98
147-151
1969
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
3
-
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-
-
-
-
-
-
2
-
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-
2
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
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