Information on EC 1.1.1.191 - indole-3-acetaldehyde reductase (NADPH)

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
1.1.1.191
-
RECOMMENDED NAME
GeneOntology No.
indole-3-acetaldehyde reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(indol-3-yl)ethanol + NADP+ = (indol-3-yl)acetaldehyde + NADPH + H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Tryptophan metabolism
-
SYSTEMATIC NAME
IUBMB Comments
(indol-3-yl)ethanol:NADP+ oxidoreductase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
indoleacetaldehyde (reduced nicotinamide adenine dinucleotide phosphate) reductase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
58875-05-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
-
very low activity
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
-
5fold higher activity than for indole-3-acetaldehyde
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
-
very low activity only for isoenzyme TH
-
?
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
-
65% of the activity compared to indole-3-acetaldehyde
-
?
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
-
2.5fold higher activity than for indole-3-acetaldehyde
-
?
butyraldehyde + NADPH
butanol + NADP+
show the reaction diagram
-
low activity
-
?
dihydroxyacetonephosphate + NADPH
glycerol 1-phosphate + NADP+
show the reaction diagram
-
well accepted substrate
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
ir
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants
-
?
indole-3-aldehyde + NADPH
indole-3-ol + NADP+
show the reaction diagram
-
19% of the activity of indole-3-acetaldehyde
-
?
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
show the reaction diagram
-
well accepted substrate
-
?
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
show the reaction diagram
-
2.4fold higher activity than for indole-3-acetaldehyde
-
?
phenylacetone + NADPH
phenylethanol + NADP+
show the reaction diagram
-
low activity
-
?
propionaldehyde + NADPH
propanol + NADP+
show the reaction diagram
-
very low activity only for isoenzyme TL
-
?
trans-cinnamaldehyde + NADPH
(2E)-3-phenylprop-2-en-1-ol + NADP+
show the reaction diagram
-
very low activity
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
ir
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
-
substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NADP+
-
with NAD+ 10times lower activity
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
29% activation at 2 mM
Fe3+
-
22% activation at 0.1 mM
Mg2+
-
29% activation at 2 mM
Mn2+
-
39% activation at 2 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Cd2+
-
complete inhibition at 2 mM
Cu2+
-
complete inhibition at 2 mM
Cu2+
-
59% inhibition at 1 mM
dihydroxyacetonephosphate
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non-competitive inhibition, Ki: 0.35 mM
EDTA
-
40% inhibition at 1 mM
Hg2+
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complete inhibition at 2 mM
Hg2+
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50% inhibition at 0.01 mM
indole-3-acetic acid
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inhibitory above 0.05 mM
Indole-3-aldehyde
-
slight inhibition at 0.2 mM
indole-3-glycolate
-
62% inhibition at 0.2 mM
iodoacetate
-
22% inhibition at 1 mM
iodoacetate
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33% inhibition at 0.1 mM
KCN
-
73% inhibition at 1 mM
KI
-
23% inhibition at 1 mM
Mn2+
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46% inhibition at 1 mM
N-ethylmaleimide
-
46% inhibition at 1 mM
NADP+
-
non-competitive inhibition, half-maximal inhibition at 0.2 mM
NADPH
-
inhibition at high concentrations
NADPH
-
inhibitory above 0.2 mM
NaF
-
35% inhibition at 1 mM
NaN3
-
slight inhibition at 1 mM
p-chloromercuribenzoate
-
17% inhibition at 0.1 mM
p-Chloromercuriphenylsulfonic acid
-
46% inhibition at 0.1 mM
p-hydroxymercuribenzoate
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40% inhibition at 0.1 mM
tryptophan
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inhibition at low concentrations
Zn2+
-
36% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
hydroxymethylindole
-
42% activation at 0.2 mM
indole-3-acetic acid
-
activation up to 0.05 mM
indole-3-ethanol
-
activation up to 0.1 mM
NaCl
-
activation up to 0.1 M
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.645
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dihydroxyacetone phosphate
-
-
0.025
-
Indole-3-acetaldehyde
-
-
0.067
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Indole-3-acetaldehyde
-
at pH 7.0, isoenzyme TH
0.125
-
Indole-3-acetaldehyde
-
-
0.125
-
Indole-3-acetaldehyde
-
-
0.133
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Indole-3-acetaldehyde
-
at pH 7.0, isoenzyme TL
0.2
-
Indole-3-acetaldehyde
-
at pH 5.2, isoenzyme TH
0.2
-
Indole-3-acetaldehyde
-
-
0.25
-
Indole-3-acetaldehyde
-
at pH 5.2, isoenzyme TL
0.011
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NADPH
-
-
0.5
-
Phenylacetaldehyde
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-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.002
-
-
partial purified from seedlings
0.014
-
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partial purified from fruits
0.04
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cytosolic fraction
0.06
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light-grown cells
0.11
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dark-grown cells
0.24
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-
purified from fruits
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.2
-
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second pH optimum at pH 7.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
maximum enzyme activity from cultures after 48 h
Manually annotated by BRENDA team
-
two isoenzymes, TH and TL
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
17000
-
-
isoenzyme TL, gel filtration
32000
-
-
gel filtration
32000
-
-
gel filtration
52000
-
-
isoenzyme TH, gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
to homogeneity, chromatography steps, native PAGE
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