Information on EC 1.1.1.191 - indole-3-acetaldehyde reductase (NADPH)

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.191
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RECOMMENDED NAME
GeneOntology No.
indole-3-acetaldehyde reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(indol-3-yl)ethanol + NADP+ = (indol-3-yl)acetaldehyde + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tryptophan metabolism
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SYSTEMATIC NAME
IUBMB Comments
(indol-3-yl)ethanol:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
58875-05-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
chinese cabbage
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
butyraldehyde + NADPH
butanol + NADP+
show the reaction diagram
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low activity
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?
dihydroxyacetonephosphate + NADPH
glycerol 1-phosphate + NADP+
show the reaction diagram
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well accepted substrate
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?
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
indole-3-aldehyde + NADPH
indole-3-ol + NADP+
show the reaction diagram
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19% of the activity of indole-3-acetaldehyde
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?
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
show the reaction diagram
phenylacetone + NADPH
phenylethanol + NADP+
show the reaction diagram
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low activity
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?
propionaldehyde + NADPH
propanol + NADP+
show the reaction diagram
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very low activity only for isoenzyme TL
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?
trans-cinnamaldehyde + NADPH
(2E)-3-phenylprop-2-en-1-ol + NADP+
show the reaction diagram
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very low activity
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
indole-3-acetaldehyde + NADPH
indole-3-ethanol + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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29% activation at 2 mM
Fe3+
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22% activation at 0.1 mM
Mg2+
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29% activation at 2 mM
Mn2+
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39% activation at 2 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cd2+
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complete inhibition at 2 mM
dihydroxyacetonephosphate
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non-competitive inhibition, Ki: 0.35 mM
EDTA
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40% inhibition at 1 mM
indole-3-acetic acid
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inhibitory above 0.05 mM
Indole-3-aldehyde
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slight inhibition at 0.2 mM
indole-3-glycolate
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62% inhibition at 0.2 mM
iodoacetate
KCN
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73% inhibition at 1 mM
KI
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23% inhibition at 1 mM
Mn2+
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46% inhibition at 1 mM
N-ethylmaleimide
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46% inhibition at 1 mM
NADP+
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non-competitive inhibition, half-maximal inhibition at 0.2 mM
NaF
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35% inhibition at 1 mM
NaN3
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slight inhibition at 1 mM
p-chloromercuribenzoate
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17% inhibition at 0.1 mM
p-Chloromercuriphenylsulfonic acid
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46% inhibition at 0.1 mM
p-hydroxymercuribenzoate
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40% inhibition at 0.1 mM
tryptophan
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inhibition at low concentrations
Zn2+
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36% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxymethylindole
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42% activation at 0.2 mM
indole-3-acetic acid
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activation up to 0.05 mM
indole-3-ethanol
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activation up to 0.1 mM
NaCl
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activation up to 0.1 M
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.715
acetaldehyde
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0.645
dihydroxyacetone phosphate
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0.025 - 0.25
Indole-3-acetaldehyde
0.011 - 0.036
NADPH
0.5
phenylacetaldehyde
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
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partial purified from seedlings
0.014
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partial purified from fruits
0.04
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cytosolic fraction
0.06
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light-grown cells
0.11
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dark-grown cells
0.24
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purified from fruits
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
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second pH optimum at pH 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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maximum enzyme activity from cultures after 48 h
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
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isoenzyme TL, gel filtration
52000
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isoenzyme TH, gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
to homogeneity, chromatography steps, native PAGE
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