BRENDA - Enzyme Database show
show all sequences of 1.1.1.191

Identification of indole-3-acetaldehyde and indole-3-acetaldehyde reductase in Chinese cabbage

Ludwig-Muller, J.; Hilgenberg, W.; Physiol. Plant. 80, 541-548 (1990)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
indole-3-acetic acid
activation up to 0.05 mM
Brassica rapa subsp. pekinensis
indole-3-ethanol
activation up to 0.1 mM
Brassica rapa subsp. pekinensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
indole-3-acetic acid
inhibitory above 0.05 mM
Brassica rapa subsp. pekinensis
iodoacetate
33% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
NADPH
inhibitory above 0.2 mM
Brassica rapa subsp. pekinensis
p-chloromercuribenzoate
17% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
p-Chloromercuriphenylsulfonic acid
46% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
p-hydroxymercuribenzoate
40% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
tryptophan
inhibition at low concentrations
Brassica rapa subsp. pekinensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
NADPH
-
Brassica rapa subsp. pekinensis
0.125
-
Indole-3-acetaldehyde
-
Brassica rapa subsp. pekinensis
0.5
-
phenylacetaldehyde
-
Brassica rapa subsp. pekinensis
0.715
-
acetaldehyde
-
Brassica rapa subsp. pekinensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Brassica rapa subsp. pekinensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Arabidopsis thaliana
-
indole-3-ethanol + NADP+
-
Arabidopsis thaliana
?
indole-3-acetaldehyde + NADPH
Brassica napus
-
indole-3-ethanol + NADP+
-
Brassica napus
?
indole-3-acetaldehyde + NADPH
Brassica oleracea
-
indole-3-ethanol + NADP+
-
Brassica oleracea
?
indole-3-acetaldehyde + NADPH
Brassica rapa subsp. pekinensis
-
indole-3-ethanol + NADP+
-
Brassica rapa subsp. pekinensis
?
indole-3-acetaldehyde + NADPH
Sinapis alba
-
indole-3-ethanol + NADP+
-
Sinapis alba
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Brassica napus
-
-
-
Brassica oleracea
-
-
-
Brassica rapa subsp. pekinensis
-
chinese cabbage
-
Sinapis alba
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Brassica rapa subsp. pekinensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
leaf
-
Brassica napus
-
leaf
-
Brassica oleracea
-
leaf
-
Brassica rapa subsp. pekinensis
-
leaf
-
Sinapis alba
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
5fold higher activity than for indole-3-acetaldehyde
286158
Brassica rapa subsp. pekinensis
ethanol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
benzaldehyde + NADPH
65% of the activity compared to indole-3-acetaldehyde
286158
Brassica rapa subsp. pekinensis
benzyl alcohol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
indole-3-acetaldehyde + NADPH
-
286158
Arabidopsis thaliana
indole-3-ethanol + NADP+
-
286158
Arabidopsis thaliana
?
indole-3-acetaldehyde + NADPH
-
286158
Brassica napus
indole-3-ethanol + NADP+
-
286158
Brassica napus
?
indole-3-acetaldehyde + NADPH
-
286158
Brassica oleracea
indole-3-ethanol + NADP+
-
286158
Brassica oleracea
?
indole-3-acetaldehyde + NADPH
-
286158
Brassica rapa subsp. pekinensis
indole-3-ethanol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
indole-3-acetaldehyde + NADPH
-
286158
Sinapis alba
indole-3-ethanol + NADP+
-
286158
Sinapis alba
?
phenylacetaldehyde + NADPH + H+
2.4fold higher activity than for indole-3-acetaldehyde
286158
Brassica rapa subsp. pekinensis
phenylethanol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Brassica rapa subsp. pekinensis
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADP+
-
Brassica napus
NADP+
-
Brassica oleracea
NADP+
-
Brassica rapa subsp. pekinensis
NADP+
-
Sinapis alba
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
indole-3-acetic acid
activation up to 0.05 mM
Brassica rapa subsp. pekinensis
indole-3-ethanol
activation up to 0.1 mM
Brassica rapa subsp. pekinensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADP+
-
Brassica napus
NADP+
-
Brassica oleracea
NADP+
-
Brassica rapa subsp. pekinensis
NADP+
-
Sinapis alba
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
indole-3-acetic acid
inhibitory above 0.05 mM
Brassica rapa subsp. pekinensis
iodoacetate
33% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
NADPH
inhibitory above 0.2 mM
Brassica rapa subsp. pekinensis
p-chloromercuribenzoate
17% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
p-Chloromercuriphenylsulfonic acid
46% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
p-hydroxymercuribenzoate
40% inhibition at 0.1 mM
Brassica rapa subsp. pekinensis
tryptophan
inhibition at low concentrations
Brassica rapa subsp. pekinensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
NADPH
-
Brassica rapa subsp. pekinensis
0.125
-
Indole-3-acetaldehyde
-
Brassica rapa subsp. pekinensis
0.5
-
phenylacetaldehyde
-
Brassica rapa subsp. pekinensis
0.715
-
acetaldehyde
-
Brassica rapa subsp. pekinensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Brassica rapa subsp. pekinensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Arabidopsis thaliana
-
indole-3-ethanol + NADP+
-
Arabidopsis thaliana
?
indole-3-acetaldehyde + NADPH
Brassica napus
-
indole-3-ethanol + NADP+
-
Brassica napus
?
indole-3-acetaldehyde + NADPH
Brassica oleracea
-
indole-3-ethanol + NADP+
-
Brassica oleracea
?
indole-3-acetaldehyde + NADPH
Brassica rapa subsp. pekinensis
-
indole-3-ethanol + NADP+
-
Brassica rapa subsp. pekinensis
?
indole-3-acetaldehyde + NADPH
Sinapis alba
-
indole-3-ethanol + NADP+
-
Sinapis alba
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Brassica rapa subsp. pekinensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
leaf
-
Brassica napus
-
leaf
-
Brassica oleracea
-
leaf
-
Brassica rapa subsp. pekinensis
-
leaf
-
Sinapis alba
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
5fold higher activity than for indole-3-acetaldehyde
286158
Brassica rapa subsp. pekinensis
ethanol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
benzaldehyde + NADPH
65% of the activity compared to indole-3-acetaldehyde
286158
Brassica rapa subsp. pekinensis
benzyl alcohol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
indole-3-acetaldehyde + NADPH
-
286158
Arabidopsis thaliana
indole-3-ethanol + NADP+
-
286158
Arabidopsis thaliana
?
indole-3-acetaldehyde + NADPH
-
286158
Brassica napus
indole-3-ethanol + NADP+
-
286158
Brassica napus
?
indole-3-acetaldehyde + NADPH
-
286158
Brassica oleracea
indole-3-ethanol + NADP+
-
286158
Brassica oleracea
?
indole-3-acetaldehyde + NADPH
-
286158
Brassica rapa subsp. pekinensis
indole-3-ethanol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
indole-3-acetaldehyde + NADPH
-
286158
Sinapis alba
indole-3-ethanol + NADP+
-
286158
Sinapis alba
?
phenylacetaldehyde + NADPH + H+
2.4fold higher activity than for indole-3-acetaldehyde
286158
Brassica rapa subsp. pekinensis
phenylethanol + NADP+
-
286158
Brassica rapa subsp. pekinensis
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Brassica rapa subsp. pekinensis
Other publictions for EC 1.1.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286157
Ludwig-Muller
-
Indole-3-acetaldehyde reductas ...
Phycomyces blakesleeanus
Physiol. Plant.
80
472-478
1990
1
-
-
-
-
-
8
3
-
1
-
1
-
1
-
-
1
-
-
1
2
-
6
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
8
-
3
-
1
-
1
-
-
-
1
-
1
2
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286158
Ludwig-Muller
-
Identification of indole-3-ace ...
Arabidopsis thaliana, Brassica napus, Brassica oleracea, Brassica rapa subsp. pekinensis, Sinapis alba
Physiol. Plant.
80
541-548
1990
2
-
-
-
-
-
7
4
-
-
1
5
-
5
-
-
1
-
-
5
-
-
8
-
-
-
-
-
1
-
-
5
-
-
-
2
-
-
5
-
-
-
-
7
-
4
-
-
1
5
-
-
-
1
-
5
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286155
Ludwig-Muller
-
Purification of NADPH-specific ...
Cucumis sativus
Physiol. Plant.
77
613-619
1989
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
2
4
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
2
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286154
Brown
Indoleacetaldehyde reductase o ...
Cucumis sativus
Plant Physiol.
65
107-113
1980
1
-
-
-
-
-
10
2
-
3
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
10
-
2
-
3
1
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286152
Bower
Auxin biogenesis. Subcellular ...
Cucumis sativus
Plant Physiol.
57
850-854
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286153
Brown
Isolation and characterization ...
Cucumis sativus
J. Biol. Chem.
251
907-913
1976
1
-
-
-
-
-
-
4
-
-
2
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
2
1
-
-
-
1
-
1
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-