BRENDA - Enzyme Database show
show all sequences of 1.1.1.191

Purification of NADPH-specific indole-3-acetaldehyde reductase from Cucumis sativus by two-dimensional native polyacrylamide gel electrophoresis

Ludwig-Muller, J.; Hilgenberg, W.; Physiol. Plant. 77, 613-619 (1989)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
dihydroxyacetonephosphate
non-competitive inhibition, Ki: 0.35 mM
Cucumis sativus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
Indole-3-acetaldehyde
-
Cucumis sativus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Cucumis sativus
substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants
indole-3-ethanol + NADP+
-
Cucumis sativus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cucumis sativus
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography steps, native PAGE
Cucumis sativus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Cucumis sativus
-
seed
-
Cucumis sativus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.002
-
partial purified from seedlings
Cucumis sativus
0.014
-
partial purified from fruits
Cucumis sativus
0.04
-
cytosolic fraction
Cucumis sativus
0.24
-
purified from fruits
Cucumis sativus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indole-3-acetaldehyde + NADPH
substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants
286155
Cucumis sativus
indole-3-ethanol + NADP+
-
286155
Cucumis sativus
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Cucumis sativus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Cucumis sativus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dihydroxyacetonephosphate
non-competitive inhibition, Ki: 0.35 mM
Cucumis sativus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
Indole-3-acetaldehyde
-
Cucumis sativus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Cucumis sativus
substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants
indole-3-ethanol + NADP+
-
Cucumis sativus
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography steps, native PAGE
Cucumis sativus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Cucumis sativus
-
seed
-
Cucumis sativus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.002
-
partial purified from seedlings
Cucumis sativus
0.014
-
partial purified from fruits
Cucumis sativus
0.04
-
cytosolic fraction
Cucumis sativus
0.24
-
purified from fruits
Cucumis sativus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indole-3-acetaldehyde + NADPH
substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants
286155
Cucumis sativus
indole-3-ethanol + NADP+
-
286155
Cucumis sativus
?
Other publictions for EC 1.1.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286157
Ludwig-Muller
-
Indole-3-acetaldehyde reductas ...
Phycomyces blakesleeanus
Physiol. Plant.
80
472-478
1990
1
-
-
-
-
-
8
3
-
1
-
1
-
1
-
-
1
-
-
1
2
-
6
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
8
-
3
-
1
-
1
-
-
-
1
-
1
2
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286158
Ludwig-Muller
-
Identification of indole-3-ace ...
Arabidopsis thaliana, Brassica napus, Brassica oleracea, Brassica rapa subsp. pekinensis, Sinapis alba
Physiol. Plant.
80
541-548
1990
2
-
-
-
-
-
7
4
-
-
1
5
-
5
-
-
1
-
-
5
-
-
8
-
-
-
-
-
1
-
-
5
-
-
-
2
-
-
5
-
-
-
-
7
-
4
-
-
1
5
-
-
-
1
-
5
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286155
Ludwig-Muller
-
Purification of NADPH-specific ...
Cucumis sativus
Physiol. Plant.
77
613-619
1989
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
2
4
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
2
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286154
Brown
Indoleacetaldehyde reductase o ...
Cucumis sativus
Plant Physiol.
65
107-113
1980
1
-
-
-
-
-
10
2
-
3
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
10
-
2
-
3
1
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286152
Bower
Auxin biogenesis. Subcellular ...
Cucumis sativus
Plant Physiol.
57
850-854
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286153
Brown
Isolation and characterization ...
Cucumis sativus
J. Biol. Chem.
251
907-913
1976
1
-
-
-
-
-
-
4
-
-
2
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
2
1
-
-
-
1
-
1
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-