BRENDA - Enzyme Database show
show all sequences of 1.1.1.191

Isolation and characterization of indole-3-acetaldehyde reductases from Cucumis sativus

Brown, H.M.; Purves, W.K.; J. Biol. Chem. 251, 907-913 (1976)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NaCl
activation up to 0.1 M
Cucumis sativus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.067
-
Indole-3-acetaldehyde
at pH 7.0, isoenzyme TH
Cucumis sativus
0.133
-
Indole-3-acetaldehyde
at pH 7.0, isoenzyme TL
Cucumis sativus
0.2
-
Indole-3-acetaldehyde
at pH 5.2, isoenzyme TH
Cucumis sativus
0.25
-
Indole-3-acetaldehyde
at pH 5.2, isoenzyme TL
Cucumis sativus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
isoenzyme TL, gel filtration
Cucumis sativus
52000
-
isoenzyme TH, gel filtration
Cucumis sativus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Cucumis sativus
-
indole-3-ethanol + NADP+
-
Cucumis sativus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cucumis sativus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Cucumis sativus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
two isoenzymes, TH and TL
Cucumis sativus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
very low activity only for isoenzyme TH
286153
Cucumis sativus
ethanol + NADP+
-
286153
Cucumis sativus
?
butyraldehyde + NADPH
low activity
286153
Cucumis sativus
butanol + NADP+
-
286153
Cucumis sativus
?
indole-3-acetaldehyde + NADPH
-
286153
Cucumis sativus
indole-3-ethanol + NADP+
-
286153
Cucumis sativus
ir
phenylacetone + NADPH
low activity
286153
Cucumis sativus
phenylethanol + NADP+
-
286153
Cucumis sativus
?
propionaldehyde + NADPH
very low activity only for isoenzyme TL
286153
Cucumis sativus
propanol + NADP+
-
286153
Cucumis sativus
?
trans-cinnamaldehyde + NADPH
very low activity
286153
Cucumis sativus
(2E)-3-phenylprop-2-en-1-ol + NADP+
-
286153
Cucumis sativus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
second pH optimum at pH 7.0
Cucumis sativus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Cucumis sativus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NaCl
activation up to 0.1 M
Cucumis sativus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Cucumis sativus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.067
-
Indole-3-acetaldehyde
at pH 7.0, isoenzyme TH
Cucumis sativus
0.133
-
Indole-3-acetaldehyde
at pH 7.0, isoenzyme TL
Cucumis sativus
0.2
-
Indole-3-acetaldehyde
at pH 5.2, isoenzyme TH
Cucumis sativus
0.25
-
Indole-3-acetaldehyde
at pH 5.2, isoenzyme TL
Cucumis sativus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
isoenzyme TL, gel filtration
Cucumis sativus
52000
-
isoenzyme TH, gel filtration
Cucumis sativus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Cucumis sativus
-
indole-3-ethanol + NADP+
-
Cucumis sativus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Cucumis sativus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
two isoenzymes, TH and TL
Cucumis sativus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
very low activity only for isoenzyme TH
286153
Cucumis sativus
ethanol + NADP+
-
286153
Cucumis sativus
?
butyraldehyde + NADPH
low activity
286153
Cucumis sativus
butanol + NADP+
-
286153
Cucumis sativus
?
indole-3-acetaldehyde + NADPH
-
286153
Cucumis sativus
indole-3-ethanol + NADP+
-
286153
Cucumis sativus
ir
phenylacetone + NADPH
low activity
286153
Cucumis sativus
phenylethanol + NADP+
-
286153
Cucumis sativus
?
propionaldehyde + NADPH
very low activity only for isoenzyme TL
286153
Cucumis sativus
propanol + NADP+
-
286153
Cucumis sativus
?
trans-cinnamaldehyde + NADPH
very low activity
286153
Cucumis sativus
(2E)-3-phenylprop-2-en-1-ol + NADP+
-
286153
Cucumis sativus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
second pH optimum at pH 7.0
Cucumis sativus
Other publictions for EC 1.1.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286157
Ludwig-Muller
-
Indole-3-acetaldehyde reductas ...
Phycomyces blakesleeanus
Physiol. Plant.
80
472-478
1990
1
-
-
-
-
-
8
3
-
1
-
1
-
1
-
-
1
-
-
1
2
-
6
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
8
-
3
-
1
-
1
-
-
-
1
-
1
2
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286158
Ludwig-Muller
-
Identification of indole-3-ace ...
Arabidopsis thaliana, Brassica napus, Brassica oleracea, Brassica rapa subsp. pekinensis, Sinapis alba
Physiol. Plant.
80
541-548
1990
2
-
-
-
-
-
7
4
-
-
1
5
-
5
-
-
1
-
-
5
-
-
8
-
-
-
-
-
1
-
-
5
-
-
-
2
-
-
5
-
-
-
-
7
-
4
-
-
1
5
-
-
-
1
-
5
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286155
Ludwig-Muller
-
Purification of NADPH-specific ...
Cucumis sativus
Physiol. Plant.
77
613-619
1989
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
2
4
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
2
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286154
Brown
Indoleacetaldehyde reductase o ...
Cucumis sativus
Plant Physiol.
65
107-113
1980
1
-
-
-
-
-
10
2
-
3
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
10
-
2
-
3
1
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286152
Bower
Auxin biogenesis. Subcellular ...
Cucumis sativus
Plant Physiol.
57
850-854
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286153
Brown
Isolation and characterization ...
Cucumis sativus
J. Biol. Chem.
251
907-913
1976
1
-
-
-
-
-
-
4
-
-
2
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
2
1
-
-
-
1
-
1
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-