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show all sequences of 1.1.1.191

Indole-3-acetaldehyde reductase in Phycomyces blakesleeanus. Characterization of the enzyme

Ludwig-Muller, J.; Schramm, P.; Hilgenberg, W.; Physiol. Plant. 80, 472-478 (1990)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
hydroxymethylindole
42% activation at 0.2 mM
Phycomyces blakesleeanus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
59% inhibition at 1 mM
Phycomyces blakesleeanus
Hg2+
50% inhibition at 0.01 mM
Phycomyces blakesleeanus
Indole-3-aldehyde
slight inhibition at 0.2 mM
Phycomyces blakesleeanus
indole-3-glycolate
62% inhibition at 0.2 mM
Phycomyces blakesleeanus
KI
23% inhibition at 1 mM
Phycomyces blakesleeanus
Mn2+
46% inhibition at 1 mM
Phycomyces blakesleeanus
NADP+
non-competitive inhibition, half-maximal inhibition at 0.2 mM
Phycomyces blakesleeanus
Zn2+
36% inhibition at 1 mM
Phycomyces blakesleeanus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADPH
-
Phycomyces blakesleeanus
0.2
-
Indole-3-acetaldehyde
-
Phycomyces blakesleeanus
0.645
-
dihydroxyacetone phosphate
-
Phycomyces blakesleeanus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe3+
22% activation at 0.1 mM
Phycomyces blakesleeanus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Phycomyces blakesleeanus
-
indole-3-ethanol + NADP+
-
Phycomyces blakesleeanus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phycomyces blakesleeanus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Phycomyces blakesleeanus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
maximum enzyme activity from cultures after 48 h
Phycomyces blakesleeanus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.06
-
light-grown cells
Phycomyces blakesleeanus
0.11
-
dark-grown cells
Phycomyces blakesleeanus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
very low activity
286157
Phycomyces blakesleeanus
ethanol + NADP+
-
286157
Phycomyces blakesleeanus
?
benzaldehyde + NADPH
2.5fold higher activity than for indole-3-acetaldehyde
286157
Phycomyces blakesleeanus
benzyl alcohol + NADP+
-
286157
Phycomyces blakesleeanus
?
dihydroxyacetonephosphate + NADPH
well accepted substrate
286157
Phycomyces blakesleeanus
glycerol 1-phosphate + NADP+
-
286157
Phycomyces blakesleeanus
?
indole-3-acetaldehyde + NADPH
-
286157
Phycomyces blakesleeanus
indole-3-ethanol + NADP+
-
286157
Phycomyces blakesleeanus
?
indole-3-aldehyde + NADPH
19% of the activity of indole-3-acetaldehyde
286157
Phycomyces blakesleeanus
indole-3-ol + NADP+
-
286157
Phycomyces blakesleeanus
?
phenylacetaldehyde + NADPH + H+
well accepted substrate
286157
Phycomyces blakesleeanus
phenylethanol + NADP+
-
286157
Phycomyces blakesleeanus
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Phycomyces blakesleeanus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8
-
Phycomyces blakesleeanus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
with NAD+ 10times lower activity
Phycomyces blakesleeanus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
hydroxymethylindole
42% activation at 0.2 mM
Phycomyces blakesleeanus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
with NAD+ 10times lower activity
Phycomyces blakesleeanus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
59% inhibition at 1 mM
Phycomyces blakesleeanus
Hg2+
50% inhibition at 0.01 mM
Phycomyces blakesleeanus
Indole-3-aldehyde
slight inhibition at 0.2 mM
Phycomyces blakesleeanus
indole-3-glycolate
62% inhibition at 0.2 mM
Phycomyces blakesleeanus
KI
23% inhibition at 1 mM
Phycomyces blakesleeanus
Mn2+
46% inhibition at 1 mM
Phycomyces blakesleeanus
NADP+
non-competitive inhibition, half-maximal inhibition at 0.2 mM
Phycomyces blakesleeanus
Zn2+
36% inhibition at 1 mM
Phycomyces blakesleeanus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADPH
-
Phycomyces blakesleeanus
0.2
-
Indole-3-acetaldehyde
-
Phycomyces blakesleeanus
0.645
-
dihydroxyacetone phosphate
-
Phycomyces blakesleeanus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe3+
22% activation at 0.1 mM
Phycomyces blakesleeanus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-acetaldehyde + NADPH
Phycomyces blakesleeanus
-
indole-3-ethanol + NADP+
-
Phycomyces blakesleeanus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Phycomyces blakesleeanus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
maximum enzyme activity from cultures after 48 h
Phycomyces blakesleeanus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.06
-
light-grown cells
Phycomyces blakesleeanus
0.11
-
dark-grown cells
Phycomyces blakesleeanus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
very low activity
286157
Phycomyces blakesleeanus
ethanol + NADP+
-
286157
Phycomyces blakesleeanus
?
benzaldehyde + NADPH
2.5fold higher activity than for indole-3-acetaldehyde
286157
Phycomyces blakesleeanus
benzyl alcohol + NADP+
-
286157
Phycomyces blakesleeanus
?
dihydroxyacetonephosphate + NADPH
well accepted substrate
286157
Phycomyces blakesleeanus
glycerol 1-phosphate + NADP+
-
286157
Phycomyces blakesleeanus
?
indole-3-acetaldehyde + NADPH
-
286157
Phycomyces blakesleeanus
indole-3-ethanol + NADP+
-
286157
Phycomyces blakesleeanus
?
indole-3-aldehyde + NADPH
19% of the activity of indole-3-acetaldehyde
286157
Phycomyces blakesleeanus
indole-3-ol + NADP+
-
286157
Phycomyces blakesleeanus
?
phenylacetaldehyde + NADPH + H+
well accepted substrate
286157
Phycomyces blakesleeanus
phenylethanol + NADP+
-
286157
Phycomyces blakesleeanus
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Phycomyces blakesleeanus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8
-
Phycomyces blakesleeanus
Other publictions for EC 1.1.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286157
Ludwig-Muller
-
Indole-3-acetaldehyde reductas ...
Phycomyces blakesleeanus
Physiol. Plant.
80
472-478
1990
1
-
-
-
-
-
8
3
-
1
-
1
-
1
-
-
1
-
-
1
2
-
6
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
8
-
3
-
1
-
1
-
-
-
1
-
1
2
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286158
Ludwig-Muller
-
Identification of indole-3-ace ...
Arabidopsis thaliana, Brassica napus, Brassica oleracea, Brassica rapa subsp. pekinensis, Sinapis alba
Physiol. Plant.
80
541-548
1990
2
-
-
-
-
-
7
4
-
-
1
5
-
5
-
-
1
-
-
5
-
-
8
-
-
-
-
-
1
-
-
5
-
-
-
2
-
-
5
-
-
-
-
7
-
4
-
-
1
5
-
-
-
1
-
5
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286155
Ludwig-Muller
-
Purification of NADPH-specific ...
Cucumis sativus
Physiol. Plant.
77
613-619
1989
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
2
4
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
2
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286154
Brown
Indoleacetaldehyde reductase o ...
Cucumis sativus
Plant Physiol.
65
107-113
1980
1
-
-
-
-
-
10
2
-
3
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
10
-
2
-
3
1
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286152
Bower
Auxin biogenesis. Subcellular ...
Cucumis sativus
Plant Physiol.
57
850-854
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286153
Brown
Isolation and characterization ...
Cucumis sativus
J. Biol. Chem.
251
907-913
1976
1
-
-
-
-
-
-
4
-
-
2
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
4
-
-
2
1
-
-
-
1
-
1
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-