6.3.5.9: hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)
This is an abbreviated version!
For detailed information about hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing), go to the full flat file.
Reaction
2 ATP + + 2 L-glutamine + 2 H2O = + 2 phosphate + + 2 L-glutamate
Synonyms
CbiA, cobalamin biosynthesis aminotransferase, CobB, cobyric acid synthase, cobyrinic acid a,c-diamide synthase, new NaMN:Me2Bza phosphoribosyltransferase enzyme
ECTree
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Turnover Number
Turnover Number on EC 6.3.5.9 - hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)
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0.16
cobyrinic acid
-
pH 7.7, wild-type enzyme
0.16
glutamine
-
pH 7.7, wild-type enzyme
0.008
-
mutant enzyme Y46A
0.04
cobyrinic acid c-monoamide
-
mutant enzyme D97N
0.057
cobyrinic acid c-monoamide
-
mutant enzyme L47A
0.2
cobyrinic acid c-monoamide
-
wild-type enzyme