6.3.5.9: hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

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For detailed information about hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing), go to the full flat file.

Reaction

2 ATP +

+ 2 L-glutamine + 2 H2O =

+ 2 phosphate +

+ 2 L-glutamate

Synonyms

CbiA, cobalamin biosynthesis aminotransferase, CobB, cobyric acid synthase, cobyrinic acid a,c-diamide synthase, new NaMN:Me2Bza phosphoribosyltransferase enzyme

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

6.3.5.9 hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

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Results	in table
6116	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
1	General Stability
28	KM Value [mM]
2	Metals/Ions
10	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
9	Organism
5	Pathway
4	pH Optimum
1	pI Value
6	Protein Variants
3	Purification (Commentary)
1	Reaction
9	Reference
2	Specific Activity [micromol/min/mg]
3	Storage Stability
36	Substrates and Products (Substrate)
7	Subunits
15	Synonyms
1	Systematic Name
8	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.3.5.9 - hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

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-20°C, 0.1 M Tris hydrochloride, pH 7.5, 100% activity is lost after 48 h

Pseudomonas denitrificans (nom. rej.)

651618

-20°C, enzyme can be stored in the eluate from purification step v without detectable loss of activity

Pseudomonas denitrificans (nom. rej.)

651618

0°C, 0.1 M Tris hydrochloride, pH 7.5, 100% activity is lost after 48 h

Pseudomonas denitrificans (nom. rej.)

651618