6.3.5.7: glutaminyl-tRNA synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about glutaminyl-tRNA synthase (glutamine-hydrolysing), go to the full flat file.

Reaction

Synonyms

aa-tRNA amidotransferase, AdT, amidotransferase B, amidotransferase C, amidotransferase, glutamyl-transfer ribonucleate (glutamine-specific), aminoacyl-tRNA amidotransferase, BANKA_112750, gatA, GatB, GatCAB, GatCAB amidotransferase, GatDE, Gln4, GlnRS, Glu-AdT, Glu-amidotransferase, Glu-tRNAGln amidotransferase, Glu-tRNAGlnAT, GluAdT GatDE, GluRS, glutamyl-tRNA(Gln) amidotransferase, glutamyl-tRNAGln amidotransferase, nondiscriminating GluRS, PBANKA_071810, PF3D7_0416100, PF3D7_0628800, Qrsl1

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

                6.3.5.7 glutaminyl-tRNA synthase (glutamine-hydrolysing)

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Results	in table
2	Activating Compound
38513	AA Sequence
1	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Cofactor
4	Crystallization (Commentary)
7	Disease/ Diagnostics
7	General Information
6	IC50 Value
24	Inhibitors
9	kcat/KM [mM/s]
15	Ki Value [mM]
26	KM Value [mM]
5	Localization
2	Metals/Ions
6	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
31	Organism
4	Pathway
5	PDB ID
2	pH Optimum
22	Protein Variants
10	Purification (Commentary)
4	Reaction
31	Reference
1	Specific Activity [micromol/min/mg]
46	Substrates and Products (Substrate)
6	Subunits
41	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
23	Turnover Number [1/s]

KM Value

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KM Value on EC 6.3.5.7 - glutaminyl-tRNA synthase (glutamine-hydrolysing)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.1 - 0.2

Asn

Bacillus subtilis

-

-

492310

0.00095

Asp-tRNAAsn

Helicobacter pylori

-

37°C, pH 7.2, amidotransferase activity

674787

0.00118

Asp-tRNAGln

Helicobacter pylori

-

37°C, pH 7.2, amidotransferase activity

674787

0.117 - 0.2068

ATP

2 entries

0.01 - 0.0509

Gln

5 entries

0.00042 - 0.0024

Glu-tRNAGln

6 entries

0.0224

L-aspartyl-tRNAAsn

Helicobacter pylori

-

37°C, pH 7.2, amidotransferase activity

674787

0.16 - 1.2

L-glutamine

2 entries

0.19 - 5.6

L-glutamyl-tRNAGln

2 entries

0.0002

L-glutamyl-tRNAGlu

Streptococcus pyogenes

-

-

492302

0.00539

tRNAGln1(UUG)

Methanothermobacter thermautotrophicus

-

pH 7.2, 37°C

728668

-

0.00133

tRNAGln2(CUG)

Methanothermobacter thermautotrophicus

-

pH 7.2, 37°C

728668

-

0.00361

tRNAGlu

Methanothermobacter thermautotrophicus

-

pH 7.2, 37°C

728668

additional information

additional information

Methanothermobacter thermautotrophicus

-

aminoacylation kinetics of enzyme variants

728668

-

0.117

ATP

Streptococcus pyogenes

-

-

492302

0.2068

ATP

Helicobacter pylori

-

37°C, pH 7.2, amidotransferase activity

674787

0.01

Gln

Bacillus subtilis

-

-

492310

0.0159

Gln

Streptococcus pyogenes

-

-

492302

0.0207

Gln

Helicobacter pylori

-

37°C, pH 7.2, amidotransferase activity

674787

0.0402

Gln

Helicobacter pylori

-

37°C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity

674787

0.0509

Gln

Helicobacter pylori

-

37°C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity

674787

0.00042

Glu-tRNAGln

Helicobacter pylori

-

in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C

728391

0.00097

Glu-tRNAGln

Helicobacter pylori

-

in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C

728391

0.00134

Glu-tRNAGln

Helicobacter pylori

-

in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C

728391

0.0017

Glu-tRNAGln

Methanothermobacter thermautotrophicus

-

37°C, pH not specified in the publication

716371

0.0023

Glu-tRNAGln

Methanothermobacter thermautotrophicus

-

37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM)

716371

0.0024

Glu-tRNAGln

Methanothermobacter thermautotrophicus

-

37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM)

716371

0.16

L-glutamine

Saccharomyces cerevisiae

P13188

wild-type, pH 7.5, 37°C

745552

1.2

L-glutamine

Saccharomyces cerevisiae

P13188

N-terminal deletion mutant, pH 7.5, 37°C

745552

0.19

L-glutamyl-tRNAGln

Saccharomyces cerevisiae

P13188

wild-type, pH 7.5, 37°C

745552

5.6

L-glutamyl-tRNAGln

Saccharomyces cerevisiae

P13188

N-terminal deletion mutant, pH 7.5, 37°C

745552