Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication | Methanothermobacter thermautotrophicus | |
0.0023 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) | Methanothermobacter thermautotrophicus | |
0.0024 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu-tRNAGln + L-glutamine | - |
Methanothermobacter thermautotrophicus | ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? | |
additional information | the association of archaeal glutamyl-tRNA synthetase (ND-GluRS) with GatDE sequesters the tRNA synthetase for Gln-tRNAGln formation, with GatDE reducing the affinity of glutamyl-tRNA synthetase (ND-GluRS) for tRNAGlu 13fold | Methanothermobacter thermautotrophicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration, GatDE forms a homodimer with the binding interface between the D-subunits | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
GatDE | - |
Methanothermobacter thermautotrophicus |
Glu-amidotransferase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication | Methanothermobacter thermautotrophicus | |
0.9 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) | Methanothermobacter thermautotrophicus | |
1.2 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) | Methanothermobacter thermautotrophicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) | Methanothermobacter thermautotrophicus | |
500 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication | Methanothermobacter thermautotrophicus | |
600 | - |
Glu-tRNAGln | 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) | Methanothermobacter thermautotrophicus |