6.3.4.18: 5-(carboxyamino)imidazole ribonucleotide synthase

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For detailed information about 5-(carboxyamino)imidazole ribonucleotide synthase, go to the full flat file.

Reaction

Synonyms

More, N5-CAIR synthetase, N5-carboxyaminoimidazole ribonucleotide synthetase, N5-carboxylaminoimidazole ribonucleotide synthetase, N5CAIR synthetase, PurK

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.4 Other carbon-nitrogen ligases

                6.3.4.18 5-(carboxyamino)imidazole ribonucleotide synthase

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Results	in table
27228	AA Sequence
2	Application
7	Cloned(Commentary)
4	Cofactor
3	Crystallization (Commentary)
3	General Information
14	IC50 Value
21	Inhibitors
19	kcat/KM [mM/s]
5	Ki Value [mM]
22	KM Value [mM]
1	Metals/Ions
3	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
21	Organism
7	Pathway
7	PDB ID
3	pH Optimum
27	Protein Variants
4	Purification (Commentary)
1	Reaction
3	Reaction Type
17	Reference
1	Specific Activity [micromol/min/mg]
13	Substrates and Products (Substrate)
4	Subunits
33	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
12	Turnover Number [1/s]

Crystallization

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Crystallization on EC 6.3.4.18 - 5-(carboxyamino)imidazole ribonucleotide synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

structures with various combinations of ATP, ADP, Mg2+, hydrogencarbonate and aminoimidazole ribonucleotide in the active site. The binding involves interactions with the conserved residues Arg272, His274 and Lys348. The formation of a carboxyphosphate intermediate through ATP phosphoryl transfer is proposed, followed by carboxylation of AIR to give the product

Bacillus anthracis

Q81ZH7

743938

hanging drop vapour diffusion of native enzyme, crystals of the native enzyme belong to space group C222(1) with unit cell dimensions of a = 93.4 A, b = 95.2 A and c = 120.6 A. Crystals of the enzyme/MgADP complex are grown by batch methods at room temperature with poly(ethylene glycol)8000 as precipitant. The crystals belong to the space group P1 with unit cell dimensions of a = 60.6 A, b = 92.1 A, c = 102.6 A, alpha = 66.1°, beta = 82.7° and gamma = 81.8°

Escherichia coli

P09029

661054

hanging drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 2000

Staphylococcus aureus

A0A0H3KFM7

726578