6.3.4.15: biotin-[biotin carboxyl-carrier protein] ligase

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For detailed information about biotin-[biotin carboxyl-carrier protein] ligase, go to the full flat file.

Reaction

Synonyms

Acetyl CoA holocarboxylase synthetase, Acetyl coenzyme A holocarboxylase synthetase, bacterial BirA biotin ligase, biotin acetyl-CoA carboxylase ligase, Biotin holoenzyme synthetase, biotin ligase, biotin protein ligase, Biotin--protein ligase, biotin-protein ligase, Biotin-[acetyl coenzyme A carboxylase] synthetase, Biotin-[acetyl-CoA carboxylase] synthetase, Biotin:apocarboxylase ligase, BirA, BirA protein, BPL, group I biotin protein ligase, HCS, Holocarboxylase synthetase, holocarboxylase synthetase 1, More, STK_15250, Synthetase, biotin-[acetyl coenzyme A carboxylase], yBL

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.4 Other carbon-nitrogen ligases

                6.3.4.15 biotin-[biotin carboxyl-carrier protein] ligase

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Results	in table
32398	AA Sequence
16	Application
1	CAS Registry Number
38	Cloned(Commentary)
68	Cofactor
14	Crystallization (Commentary)
24	Disease/ Diagnostics
16	General Information
3	IC50 Value
28	Inhibitors
13	kcat/KM [mM/s]
13	Ki Value [mM]
53	KM Value [mM]
10	Localization
30	Metals/Ions
17	Molecular Weight [Da]
52	Natural Substrates/ Products (Substrates)
411	Organism
5	Pathway
94	PDB ID
9	pH Optimum
68	Protein Variants
31	Purification (Commentary)
4	Reaction
3	Reaction Type
99	Reference
15	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
149	Substrates and Products (Substrate)
30	Subunits
140	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
6	Temperature Stability [°C]
39	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.3.4.15 - biotin-[biotin carboxyl-carrier protein] ligase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-80°C, 1-2 mg/ml purified recombinant His6-tagged enzyme, in 50 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, pH 8.0, 250 mM NaCl, at least 6 months without loss of activity

Staphylococcus aureus

-

690291

4°C, immobilized recombinant enzyme, PBS, completely stable for 4 weeks, loss of activity after 5 months

Escherichia coli

-

664436