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6.3.4.15: biotin-[biotin carboxyl-carrier protein] ligase

This is an abbreviated version!
For detailed information about biotin-[biotin carboxyl-carrier protein] ligase, go to the full flat file.

Word Map on EC 6.3.4.15

Reaction

ATP
+
biotin
 +
[biotin carboxyl-carrier protein]-L-lysine
=
AMP
 +
diphosphate
 +
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine

Synonyms

Acetyl CoA holocarboxylase synthetase, Acetyl coenzyme A holocarboxylase synthetase, bacterial BirA biotin ligase, biotin acetyl-CoA carboxylase ligase, Biotin holoenzyme synthetase, biotin ligase, biotin protein ligase, Biotin--protein ligase, biotin-protein ligase, Biotin-[acetyl coenzyme A carboxylase] synthetase, Biotin-[acetyl-CoA carboxylase] synthetase, Biotin:apocarboxylase ligase, BirA, BirA protein, BPL, group I biotin protein ligase, HCS, Holocarboxylase synthetase, holocarboxylase synthetase 1, More, STK_15250, Synthetase, biotin-[acetyl coenzyme A carboxylase], yBL

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.15 biotin-[biotin carboxyl-carrier protein] ligase

Cloned

Cloned on EC 6.3.4.15 - biotin-[biotin carboxyl-carrier protein] ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coding region of birA biotin-protein ligase gene cloned from genomic DNA by PCR, verfied by sequencing and expressed in mouse erythroleukemic cells
-
codon-optimized expression in Escherichia coli
construction and expression of the C-terminal 87 amino acids of biotin carboxyl carrier protein, i.e. BCCP-87. cross-species activity of BPLs permitts functional cloning of the cDNA for yeast BPL through genetic complementation with a conditional lethal birA- strain of Escherichia coli
cross-species activity of BPLs permitts functional cloning of the cDNA for human BPL through genetic complementation with a conditional lethal birA? strain of Escherichia coli
-
cross-species activity of BPLs permitts functional cloning of the cDNA for yeast BPL through genetic complementation with a conditional lethal birA? strain of Escherichia coli
-
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21
-
doxycycline-regulated expression of a secreted variant of Escherichia coli biotin ligase BirA with target proteins containing a 13-residue biotin acceptor peptide appended to their extracellular domain, co-expression with three different G-protein coupled receptors, i.e. protease-activated receptors 1 and 2, and the platelet ADP receptor, P2Y12, which become biotinylated, in CHO-K1 Tet-On cells on the cell surface, overview
-
expressed as thioredoxin- and maltose binding protein-fusion proteins in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli DH5alpha cells
expression as His6-tagged enzyme in strain BL21(DE3)
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli strain JM109
expression in Escherichia coli strains DH5alpha and BL21(DE3)
-
expression of BirA from pET-based vector pHBA in Escherichia coli BL21 (lambdaDE3)
-
expression of HCS in Spodoptera frugiperda Sf9 cells and in Hi5 insect cells using the baculovirus transfection system
-
expression of His9-tagged BPL in Saccharomyces cerevisiae strain w303, functional complementation of the conditional lethal birA85 Escherichia coli strain BM4062
-
gene birA, expression of His-tagged wild-type and mutant enzymes in strain JM109, expression of enzyme in strain BM4092 unbiotinylated form
-
gene birA, functional expression of N-terminally and C-terminally multiply tagged enzyme in Saccharomyces cerevisiae, low level expression of the surface-displayed enzyme, expression of avitagged-BirA in Saccharomyces cerevisiae, overview
-
gene Rv3279c, DNA and amino acid sequence determination and analysis, expression of C-terminally Strep-tagged BirA in Escherichia coli
overexpression in Escherichia coli
overexpression of the enzyme comprising residues 1-125 in Escherichia coli strain BL21
-