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6.3.1.5: NAD+ synthase

This is an abbreviated version!
For detailed information about NAD+ synthase, go to the full flat file.

Word Map on EC 6.3.1.5

Reaction

ATP
+
deamido-NAD+
+
NH3
=
AMP
+
diphosphate
+
NAD+

Synonyms

ammonia-dependent NADS, ammonia-dependent nicotinamide adenine dinucleotide synthetase, ammonia-specific NAD synthase, ammonia-specific NAD synthetase, Diphosphopyridine nucleotide synthetase, draNADS, MJ1352, mj_NADS, NAD synthase, NAD synthetase, NAD+ synthetase, NadE, NADS, NH3-dependent NAD+ synthetase, Nicotinamide adenine dinucleotide synthetase, Qns1, Synthetase, nicotinamide adenine dinucleotide

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.5 NAD+ synthase

Engineering

Engineering on EC 6.3.1.5 - NAD+ synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D593A
-
poor synthetase activity
D593A/F622A
-
synthetase dead
E177A
-
poor synthetase activity
F622A
-
poor synthetase activity
I111A
-
poor synthetase activity
L529A/L604A
-
poor synthetase activity
L604A
-
poor synthetase activity
L604N
-
poor synthetase activity
M621A
-
synthetase mutant that depresses all activities
R112L
-
poor synthetase activity that inhibit substrate synergism
R112S
-
poor synthetase activity that inhibit substrate synergism
Y532A/Y601A
-
synthetase dead
Y601A
-
poor synthetase activity
Y601A/M621A
-
synthetase dead
additional information