6.3.1.5: NAD+ synthase

This is an abbreviated version!
For detailed information about NAD+ synthase, go to the full flat file.

Word Map on EC 6.3.1.5

6.3.1.5

adenylyltransferase

nmnat

glutamine-dependent

nucleolus-associated

analysis

pharmacology

drug development

Reaction

Synonyms

ammonia-dependent NADS, ammonia-dependent nicotinamide adenine dinucleotide synthetase, ammonia-specific NAD synthase, ammonia-specific NAD synthetase, Diphosphopyridine nucleotide synthetase, draNADS, MJ1352, mj_NADS, NAD synthase, NAD synthetase, NAD+ synthetase, NadE, NADS, NH3-dependent NAD+ synthetase, Nicotinamide adenine dinucleotide synthetase, Qns1, Synthetase, nicotinamide adenine dinucleotide

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

6.3.1.5 NAD+ synthase

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Results	in table
21298	AA Sequence
10	Application
1	CAS Registry Number
15	Cloned(Commentary)
16	Cofactor
12	Crystallization (Commentary)
11	General Information
12	IC50 Value
26	Inhibitors
3	kcat/KM [mM/s]
22	KM Value [mM]
2	Localization
15	Metals/Ions
6	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
227	Organism
9	Pathway
49	PDB ID
8	pH Optimum
3	pH Range
1	pI Value
16	Protein Variants
16	Purification (Commentary)
4	Reaction
19	Reaction Type
40	Reference
1	Source Tissue
24	Specific Activity [micromol/min/mg]
4	Storage Stability
61	Substrates and Products (Substrate)
11	Subunits
77	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
4	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 6.3.1.5 - NAD+ synthase

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3 entries

native enzyme to homogeneity

Geobacillus stearothermophilus

E07950

650637

Ni HiTrap column chromatography, and Superdex-200 gel filtration or Superdex-75 gel filtration

Bacillus anthracis

Q81RP3

671062

Ni-NTA Superflow resin chromatography

Staphylococcus aureus

674946

recombinant as His-tagged enzyme from Escherichia coli

Streptococcus sobrinus

651734

recombinant enzyme from Escherichia coli, over 95% purity

Bacillus subtilis

649168

recombinant His-tagged enzyme and recombinant thioredoxin-His6-fusion protein from Escherichia coli and insect cells, the His-tag is cleaved off afterwards

Mycobacterium tuberculosis

653750

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Staphylococcus aureus

Q5HEK9

743959

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) star pLysS by nickel affinity chromatography, tag cleavage by a protease, another step of nickel affinity chromatography, and gel filtration

Deinococcus radiodurans

Q9RYV5

744619

recombinantly overexpressed enzyme, to homogeneity

Escherichia coli

653053