6.3.1.5: NAD+ synthase
This is an abbreviated version!
For detailed information about NAD+ synthase, go to the full flat file.
Word Map on EC 6.3.1.5
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6.3.1.5
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adenylyltransferase
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nmnat
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glutamine-dependent
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nucleolus-associated
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analysis
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pharmacology
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drug development
- 6.3.1.5
- adenylyltransferase
- nmnat
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glutamine-dependent
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nucleolus-associated
- analysis
- pharmacology
- drug development
Reaction
Synonyms
ammonia-dependent NADS, ammonia-dependent nicotinamide adenine dinucleotide synthetase, ammonia-specific NAD synthase, ammonia-specific NAD synthetase, Diphosphopyridine nucleotide synthetase, draNADS, MJ1352, mj_NADS, NAD synthase, NAD synthetase, NAD+ synthetase, NadE, NADS, NH3-dependent NAD+ synthetase, Nicotinamide adenine dinucleotide synthetase, Qns1, Synthetase, nicotinamide adenine dinucleotide
ECTree
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Specific Activity
Specific Activity on EC 6.3.1.5 - NAD+ synthase
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0.0003
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mutant enzyme R112L, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.0011
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mutant enzyme D593A/F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.0027
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mutant enzyme L604A/L519A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.0033
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mutant enzyme Y532A/M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.0043
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mutant enzyme L604N, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.008
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mutant enzyme F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.009
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partially purified recombinant His-tagged enzyme expressed in insect cells, substrate NH4+
0.0143
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mutant enzyme I111A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.015
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mutant enzyme R112S, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.033
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purified recombinant HIs-tagged enzyme expressed in Escherichia coli, substrate glutamine
0.0485
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mutant enzyme Y601A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.1
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purified recombinant His-tagged enzyme expressed in Escherichia coli, substrate NH4+
0.152
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mutant enzyme D593A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.183
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mutant enzyme L604A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.25
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mutant enzyme M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.631
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mutant enzyme E177A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin
0.67
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purified recombinant His-tagged truncated enzyme expressed in Escherichia coli, substrate NH4+
0.741
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wild type enzyme, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin