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6.1.1.24: glutamate-tRNAGln ligase

This is an abbreviated version!
For detailed information about glutamate-tRNAGln ligase, go to the full flat file.

Word Map on EC 6.1.1.24

Reaction

ATP
+
L-glutamate
 +
tRNAGlx
=
AMP
 +
diphosphate
 +
glutamyl-tRNAGlx

Synonyms

Glu-Q-RS, GluRS, GluRS1, GluRS2, GluRSND, glutamyl-queuosine tRNAAsp synthetase, Glutamyl-tRNA synthetase, GlxRS, ND-GluRS, non-discriminating GluRS, non-discriminating glutamyl-tRNA synthetase, nondiscriminating GluRS, nondiscriminating glutamyl-tRNA synthetase, Pf3D7_1357200, TM1875

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.24 glutamate-tRNAGln ligase

Cloned

Cloned on EC 6.1.1.24 - glutamate-tRNAGln ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Bacillus subtilis GluRS-dependent Glu-tRNAGln formation may cause growth inhibition in the transformed Escherichia coli strain, possibly due to abnormal protein synthesis
-
expressed in Escherichia coli
expression in Escherichia coli
expression of C-terminally and N-terminally His6-tagged ND-GluRS in Escherichia coli strain BL21
Thermosynechococcus vestitus
-
expression of C-terminally His-tagged mutant enzymes containing an N-terminal signal sequence tag directing the protein to the periplasm, without effect on the steady-state kinetic parameters of GlnRS. The mutants are expressed as N-terminal fusions with the leader sequence of the bacterial fd gene III protein in Escherichia coli
-
expression of Glu-Q-RS in Escherichia coli strain BL21(DE3)
-
expression of GluRSND using a pCYB1 vector in Escherichia coli pLysS Rosetta cells
-
gene Pf3D7_1357200, recombinant expression of codon-optimized GluRS in Escherichia coli KRX cells
ND-GluRS, phylogenetic analysis
-
the gene is cloned with its sigmaA promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and bacillus subtilis. Transformation of Bacillus subtilis with this recombinant plasmidleads to a 30fold increase of glutamyl-tRNA synthetase specific activity in crude extracts. Transformation of Escherichia coli with this plasmid gives no recombinants. The presence of Bacillus subtilis glutamyl-tRNA synthetase is lethal for Escherichia coli, probably because this enzyme glutamylates tRNA1Gln in vivo as it does in vitro
-
TM1875, sequence comparisons, expression in Escherichia coli strain Rosetta2(DE3)